"The world is a mess, and I just...need to rule it."
Adorkable: He can't talk to girls, he thinks that Captain Hammer and Penny are "probably going to French kiss or something" and is played by Neil Patrick Harris.
Thou Shalt Not Kill: Personally against murder, which he sees as beneath him—or at least, would like to.
It seems to be implied that the real reason he won't kill is because he knows it's wrong, but he doesn't want to admit that, so makes up an excuse about it not being "elegant or creative" enough.
Drop the Hammer: Averted; despite having the word 'hammer' in his name, he's never actually seen using one as a weapon.
Dumb Is Good: Advocates anti-intellectualism, telling kids that any kid who's either a nerd or "different" in some ways is a potential supervillain who must be put behind bars.
Dumb Muscle: Downplayed in that he's smart enough to follow Dr. Horrible's Blog and immediately realizes that Billy is Dr. Horrible.
Miles Gloriosus: He's only brave and bold because he's invulnerable to damage. The first time he gets hurt, he literally runs screaming like a little girl and spends months in therapy over it.
Neat Freak: Why he constantly wears gloves. Pay attention to him during the "My Eyes" song and it becomes clear he's hiding it from Penny. Possibly just an extension of his superiority issues over everyone else.
Though it's more implied that it's because no woman would ever want to a second time.
It also could be that he was the type of jerk who'd have no use for a woman once he'd slept with her, and just somehow seems to think Penny is different.
He just sees his relationship with her as a way to torment Billy.
Science Is Bad: In the spinoff comic, he demands that children report nerds to the police.
The Unintelligible: Subverted. Speaks only in horse sounds for most of the ELE comic, with Jefferson claiming that Bad Horse was appointing him "vice president." Subverted at the very end when Bad Horse speaks English.
Professor Normal: A cyborg with a super strong robotic arm and jaw attachment. Otherwise, he's a normal person with a wife, kids, and a mortgage, hence his name.
Tie-Die: A hippy-themed supervillainess able to make hippies violent and angry.
Dead Bowie: A David Bowie-inspired supervillain with the power of persuasion. Claims to be the real David Bowie who was murdered in 1980 and that every other appearance by Bowie afterward was an impostor.
Fury Leika: Her name is a pun off of "Hell hath no fury like a Woman Scorned." She was left at the altar and then pooped on by a radioactive dove. She gained poisonous flowers, incendiary rice, a ball and chain around her ankle, and four henchwomen each carrying something old, something new, something borrowed, and something blue.
Fake Thomas Jefferson: Claims to be the real Thomas Jefferson, though that is unlikely unless Thomas Jefferson had Super Strength. Also carries a quill pen tipped with tranquilizer. Doesn't like to have lots of stuff in his pockets.
Snake Bite: A supervillainess with a cobra-themed costume. Carries a staff topped by a mini disco ball. Did not appear in the "Evil League of Evil" comic with the other ELE leaders.
Minor villains
Hourglass: A villainess who is able to see the future. Tells Moist about a kid who will become President. An unseen character.
Bait and Switch: A pair of villains who Moist goes on a double date with. Somehow able to fool him into thinking he was dating Bait instead of Switch. Bait is apparently the more attractive of the two.
Conflict Diamond: A villain friend of Dr. Horrible and Moist. Went on the double date with Moist, Bait, and Switch.
Pink Pummeler: A homophobic villain with Super Strength, Gaydar, and a superhuman knowledge of Broadway show tunes. Repeatedly rejected by the ELE due to his tendency to date rather than beat up his victims.
Purple Pimp: A supervillain who appears at the party for Dr. Horrible's induction into the ELE.
Minor heroes
Johnny Snow: An amateur hero not affiliated with the Council of Champions. He wears a parka and carries an Ice Beam. He's mentioned in Act I and appears in the ELE comics, where he foils a plot by the ELE to poison the city's water supply by freezing it. The villains are impressed and mistake him for a supervillain.
James Flames: A potbellied superhero with pyrokinetic powers. His Catch Phrase is "M'name's James and I have the power of flames." Wears a singed T-shirt and pink overalls. He thaws the water supply after Johnny Snow freezes it.
Wingspan: A member of the Council of Champions, along with Captain Hammer. Works in a command center in the sky. He, along with the rest of the Council, foolishly leaves the city for a retreat, allowing the ELE to wreak havoc. Appears briefly in the ELE comic.
Elementia: A blue-skinned superheroine and a member of the Council of Champions. Works in a command center with a waterfall and apparently has some kind of Elemental Powers. Apparently has no problem sitting naked in a sauna with men.