Two playful chipmunks who live in an oak tree at the edge of the forest and spend their time in equal parts gathering nuts, avoiding predators and making mischief, their favorite prank target being Donald Duck (making them more tied to the Disney Ducks Comic Universe).
You No Take Candle: Since their sped-up, near-unintelligible voices was impossible to recreate for comics, several older comics try to recreate a similar effect by having the chipmunks speak in broken English similar to how the mice in Cinderella spoke. This was pretty much abandoned in later comics.
Zeke Midas "Big Bad" Wolf
The wannabe "terror of the forest," Zeke Wolf has two main goals in life: To catch and eat the Three Little Pigs, and to raise his son to become a proper bad wolf. So far, his success rate in both these goals is zero — though not for lack of trying.
Villain Protagonist: Though he's rarely the titular character of his comics, he's most definitely the star of most of them, getting far more screen-time than his son or the pigs.
Zeke's do-gooder son, and the "white sheep" of the wolf family; despite his father's constant attempts at making him into a "proper" wolf, he's a polite, kind and considerate guy who just doesn't have it in him to be bad or nasty.
Divergent Character Evolution: From the cartoons. In the early cartoons, Zeke had three sons, all as bad as their father — though later adaptations changed this to two bad little wolves and one good little wolf. Eventually, as the comic series found its feet, the two bad little wolves vanished from the stories, leaving only Li'l Wolf as Zeke's only son.
Fifer, Fiddler and Practical Pig are the best friends of Li'l Wolf and constant target of his father Zeke.
Foolish Sibling, Responsible Sibling: The All Work VS All Play trope is much less prominent in the comics than in the cartoons, as the pigs work together to a much higher degree in order to defend themselves from the wolf. However, there's still no doubt that Practical is by far the smartest pig, and the one who most often has to save Fifer and Fiddler, or clean up their messes when they've screwed up.
Gadgeteer Genius: Practical. Unsurprisingly, all his gadgets are based around defense against the Big Bad Wolf.
Single-Minded Twins: Fifer and Fiddler don't speak in unison the way they do in the cartoon, but they still have the exact same personality and are hardly ever seen apart.
Vague Age: They live on their own and take care of themselves, yet they go to school together with Li'l Wolf and are often treated as children. Some comics have Practical as the sole adult pig who for some reason acts as the guardian to his younger brothers.
"Br'er" Riley Rabbit
Known almost exclusively as "Br'er Rabbit," he is a clever, mischievous rabbit who always has a trick or two up his sleeve, but often gets himself into trouble thanks to his impulsive, braggart nature.
Jerk with a Heart of Gold: He's a prankster and an annoying braggart who will occasionally dish out more punishment to the bad guys than they really deserve. Depending on the Writer just how much of a jerk he's willing to be (in the earlier stories he could occasionally come across as a Designated Hero), but generally he's not a bad person.
Mr. Vice Guy: His main vice is arrogance and a tendency towards self-centeredness.
Distressed Damsel: In some comic stories, in that she fulfills much the same role for Br'er Rabbit as Minnie Mouse does for Mickey Mouse... of course, at least the bad guys who captured Minnie weren't usually intent on eating her.
The only permanent, and definitely the smartest, member of the "Foul Fellows' Club;" he's primarily known as a troublemaker, a rascal and a crook who's always up to no good. Forms the Brains part in a Brains and Brawn duo with Br'er Bear, and occasionally teams up with Zeke Wolf.
Cunning Like a Fox: Though he's not quite as cunning as he himself thinks, he is by far the smartest person in the Foul Fellows' Club and can out-think both Zeke Wolf and Br'er Bear without even trying.
Evil Counterpart / Foil / Shadow Archetype: To Br'er Rabbit. They have pretty much the same strengths and the same flaws, the same sense of humor, the same gift for tricks and cunning... and the same tendency to think better of themselves than they really deserve. The main difference is that Br'er Rabbit is at worst a Jerk with a Heart of Gold, while Br'er Fox delights in being wicked and sadistic... and Br'er Rabbit is, when all is said and done, just a little smarter than Br'er Fox is.
Those Two Bad Guys: Sometimes with Br'er Bear, occasionally with Zeke Wolf. Not that he has much respect for either of them; he considers Zeke a miserable failure and Br'er Bear a blundering idiot.
Br'er Fox's sometimes-partner-in-crime, and constant bane of Zeke Wolf's scheme, he's not so much a bad guy as he is overly temperamental and easily swayed thanks to his less-than-stellar intelligence. Gets along famously with Li'l Wolf and the pigs, but wouldn't pass up a chance to knock Br'er Rabbit's head clean off.
Bears Are Bad News: Though he's definitely worse news for some characters than for others.
Composite Character: In the very earliest pig/wolf comics, Zeke's nemesis was an Expy of Br'er Bear named "Farmer Bear," who looked and mostly acted like Br'er Bear but without the Funetik Aksent and the occupation as, well, a farmer. It wasn't long before the decision was made to just use Br'er Bear in these stories, hence Br'er Bear took over Farmer Bear's role and farm. So, interestingly enough, B'rer Bear is a Composite Character of himself and his own Expy.
Depending on the Writer: Is he a good guy or a bad guy? If you see him with the Three Little Pigs, he's usually a good guy — with Br'er Rabbit he's usually a bad guy. Early stories actually explored this a little, explaining that he was nice to the pigs because they were nice to him but detested the rabbit who was always tricking and mocking him. Some later writers/stories thought this switching good guy/bad guy status was too confusing and made him more of a temperamental good guy, even being on friendly terms with Br'er Rabbit.
Shot in the Ass: He's got a shotgun... and is notable for being the one Disney comic character who actually hits the one he's shooting at. Which is usually Zeke, and always this trope.
An evil inventor who switches between being a thorn in the side of both Ducks and Mice; he's Gyro Gearloose's main (and unscrupulous) rival but also a prominent villain in Mickey Mouse and Super Goof stories. He's a villain in the Ultraheroes comic.
Always Someone Better: He never manages to reach up to the levels reached by Gyro Gearloose, and obsesses quite a bit over this.
Evil Counterpart / Foil / Shadow Archetype: To Gyro. He's smart, for sure, but not as smart, and where Gyro is humble, hard-working and honest, Emil is boastful, likely to take shortcuts with his work and won't hesitate to cheat, steal or take credit for inventions that aren't actually his.
Expy: In the Super Goof comics, he's a clear one for Lex Luthor. In fact, When Super Goof was reintroduced in Italian stories in 1999, Emil re-emerged as a Corrupt Corporate Executive in the vein of the modern Luthor.
Mad Scientist: Possibly why he's such a popular foe for Super Goof and why he got so prominent in the Ultraheroes comic.
Rogues-Gallery Transplant: He originally appeared as Gyro Gearloose's rival in the Duck comics, but later crossed into Mickey Mouse stories; there he served as the source of equipment that villains like Pete would use, despite them clearly not being smart enough to create it.
Shorter Means Smarter: Played with and ultimately averted. He is short, and certainly smart, and a brilliant inventor in his own right, but he's nowhere near as brilliant as Gyro, who's much taller than him.
Wild Card: Whether she is good or evil seems almost entirely up to chance sometimes. She has teamed up with just about every other Disney comic villain, from Magica De Spell to the Beagle Boys to the Phantom Blot, and even Captain Hook, but it seems more like a whim and less like genuine villainy (and often she works with them because the harbors a crush on one of them), and she probably has more stories where she's a good guy.
Witch Hazel and Beelzebub
Hazel is another witch, a lot more traditional-minded than Madam Mim, but like Mim she's not all that evil when it gets down to it. In later years she seems to spend an awful lot of time trying to convinse Flat Earth AtheistGoofy that she is, in fact, a witch. Her constant companion, and transportation device, is a sentient, living broomstick called Beelzebub.
Friend to All Children: One of the biggest differences between her and the Witch Hazel from the Looney Tunes franchise is that she adores children and often goes out of her way to help them against mean or unfair adults.
The Gadfly: Her main mode of operation as a "wicked witch" is to use her magic to basically Troll unsuspecting humans.