'''The Biomen'''

![[color:red:Gou Shiro/Red One]]

* AcePilot
* FriendToAllLivingThings
* LawOfChromaticSuperiority: Shirou can understand animals, but nobody else is shown to have unmorphed powers. [[spoiler: He's also the only one to be linked to the Dr. Man part of the plot through his father.]]
* TheLeader
* PlayingWithFire: [[FlamingSword Fire Sword]]

![[color:green:Takasugi Shingo/Green Two]]

* BadassDriver
* TheBigGuy: Up until #10.
* BlowYouAway: Hurricane Sword
%%* GeniusBruiser
* TheLancer: He is the NumberTwo after all, but he really cements himself in this position once Mika bites it.
%%* PrecisionGuidedBoomerang: Green Bomerang
%%* TheSmartGuy

![[color:blue:Nanbara Ryuuta/Blue Three]]

%%* FragileSpeedster
%%* KidAppealCharacter
* PunnyName: His Bioman codename is a pun on how the Japanese pronounce "Bruce Lee".
%%* ShockAndAwe: Elec Sword

![[color:gold:Koizumi Mika/Yellow Four I]]

%%* ActionGirl
%%* BikerBabe
* FourIsDeath: Played quite straight.
%%* HeroicSacrifice
* HotScoop
* KilledOffForReal
* TheLancer: She was the most hesitant to be part of the Bio Team.
* LittleDeadRidingHood: Subverted that she died as Yellow Four, but she wears red as casual clothes so...
* RealLifeWritesThePlot: The main writer had plans for Mika that [[WhatCouldHaveBeen ultimately went unrealized]] after the actress left the series so early.
* ShockAndAwe: Thunder Sword
* TakingTheBullet
* TomboyAndGirlyGirl: Tomboy to Hikaru's Girly Girl. (Originally)

![[color:gold:Yabuki Jun/Yellow Four II]]

* ActionGirl: Very much so, especially since her actress did her own stunts.
* TheBigGuy: By virtue of being the ActionGirl.
* LegacyCharacter
* MartialArtsHeadband: You don't ever see Jun without wearing one (and it fits [[TheBigGuy her role]] too). [[spoiler:Even one episode had this weaponized by the villains by replacing it with a time bomb!]]
* ShockAndAwe: Thunder Sword
* {{Shorttank}}
* TomboyAndGirlyGirl: Tomboy to Hikaru's Girly Girl. (Replacement)
* [[WhyDidItHaveToBeSnakes Why Did It Have to Be Spiders?]]

![[color:fuchsia:Katsuragi Hikaru/Pink Five]]

* BarrierWarrior: Pink Barrier
* TheChick
* HolyHandGrenade: [[FrickinLaserBeams Laser Sword]]
* TenderTears: She's the most emotional of the Biomen and tends to cry easily.
* TomboyAndGirlyGirl: Girly Girl to both Yellow Four's Tomboy.

* GenderFlip: Even though he's technically a sexless automaton, he addresses himself as a male in the Japanese original. In the English dub however, he was made female.
* RobotBuddy

!Bio Robo
* CombiningMecha: Composed of two jets (Bio Jet 1 and Bio Jet 2).
* HumongousMecha
* MechanicalLifeform: It is shown that Bio Robo has a will of its own when it runs away during Silva's first appearance.

'''Neo Gear Empire'''
!Doctor Man
* BigBad
* MorallyAmbiguousDoctorate: That 'Doctor' title certainly doesn't come from medical expertise.
* WasOnceAMan

* TheDragon
* TheStarscream: This is implied to have been the case in the series' past; though never implemented on-screen.

* EvilGenius
* MadScientist

* TheBrute
* ButtMonkey

'''Anti-Bio Union'''
!Bio Hunter Silva
* BigBad: He reappears as one of the main antagonists of Film/KamenRiderXSuperSentaiSuperHeroTaisen.
* BladeBelowTheShoulder: Both which can be fired like arrows.
* CatchPhrase: Hakai! Hakai! Hakai! (Destroy! Destroy! Destroy!)
* TheDreaded: He possesses Anti-Bio Particles that are more powerful than those used [[spoiler:to kill the first Yellow Four]] (Even though he doesn't manage to kill any Biomen with them). Peebo had a very good reason to fear him.
* EvilLaugh
* {{Expy}}: Based on Series/{{Kikaider}}'s nemesis Hakaider.
* HeroKiller: Less on the Killer part, but he does put up one hell of a fight.
* HumongousMecha: Pilots one.
* WildCard