Voiced by: John TravoltaThe main and titular protagonist.
- Amplified Animal Aptitude: On and off the set.
- Ass Kicking Pose: Before delivering the Superbark, Bolt always assumes the position. Right forepaw, forward. Right hindpaw, backward. Then, WOOOOF!!!
- Badass Adorable: In the TV series. He's actually an adorable shepherd.
- Canine Companion: To Penny. First in the Show Within a Show, and then in real life at the end.
- The Comically Serious: Due to him actually thinking he's a superhero.
- Conspiracy Theorist: Thinks Dr. Calico, the villain of the Show Within a Show, is behind everything.
- Determinator: He will not give up searching for Penny no matter what.
- Female Feline, Male Mutt: With Mittens.
- Fish out of Water: Has spent most of his life being tricked into thinking that his superhero action show is reality, and that he actually has superpowers. Outside of his controlled soundstage, he has no idea what is going on and tends to attempt things that he is not capable of.
- Heroic Dog: Spends most of his life being tricked into thinking he's a superhero. Even after he realizes it was all fake, he continues to act as a hero, with his daring rescue of Mittens from the pound, and later on trying to save Penny from a burning building.
- Interspecies Friendship: With Mittens and Rhino.
- Large Ham: comes as a package with the superhero delusions.
- Locked into Strangeness: Played with. He believes the lightning mark in his fur is a symbol of his powers, but it's actually just make up.
- No Social Skills: Having spent 5 years isolated in the TV studio, Bolt is often socially inept when meeting and interacting with different characters in reality, such as Mittens and other dogs. Mittens, who is a cat, actually knows more about how dogs are supposed to behave around each other than he does, and has to teach him.
- Platonic Life Partners: Ends being extremely close friends with Mittens.
- Ridiculously Cute Critter: As a puppy.
- Stock Superpowers: Has these on the Show Within a Show. Spends most of the movie thinking he actually has these powers.
- Talking Animal: Only other animals can understand him, though; he just sounds like a normal dog to humans.
- Truman Show Plot: Spends most of his life thinking he is actually a superhero, and the plot of the TV show he stars in is reality.
- Undying Loyalty: Raised from puppyhood to be Penny's protector.
Voiced by: Susie EssmanA stray cat that Bolt befriends on his journey to find Penny.
- Balloon Belly: After having Bolt beg for food from the people in the RVs. She loved every minute of it.
- Broken Bird: She's been traumatized by her Dark and Troubled Past, and it's made her bitter and cynical.
- Cats Are Mean: Subverted. Initially, she kicks around the pigeons and makes them bring her food, but during her journey with Bolt and Rhino, she softens up and grows a heart.
- Cats Are Snarkers: Responds to Bolt's superhero delusions, among other things, with much sarcasm. She's the page picture for a reason.
- Cat Stereotype: She's black and white, so she's snarky.
- Con Man: Since she's been declawed, she can't hunt. But she likes to threaten people with her claws that don't actually exist. Before she met Bolt, she survived by extorting food from pigeons in this way, by making empty threats to the pigeons of, "bring me food or I eat you."
- Dark and Troubled Past: Her owners suddenly packed up and moved away one day, leaving her, a declawed cat, to fend for herself. She wonders what she could have possibly "done wrong" to cause them to abandon her.
- Defrosting Ice Queen: Cold and selfish, but ends up warming up to Bolt. Eventually.
- Female Feline, Male Mutt: With Bolt.
- Freudian Excuse: The reason she's a jerk who runs a protection racket against pigeons for a living, is because her owners abandoned her.
- Green Eyes
- Interspecies Friendship: With Bolt and Rhino.
- Jerk with a Heart of Gold: She's not very nice, but underneath it all she's just desperate and lonely.
- Lean and Mean: At first.
- The Not-Love Interest: Though they're never romantically involved, her plot arc with Bolt has a lot of the hallmarks of a romantic subplot, complete with a pseudo-Falling in Love Montage. By the end, they're basically Platonic Life Partners.
- Only Sane Woman: Amongst herself, Bolt, and Rhino, she's the only one who knows that Bolt's TV show isn't real. When Rhino mentions seeing Bolt perform amazing feats on the "magic box", she immediately pieces together that he's a TV star.
- The Pigpen: Is rather dirty and scruffy-looking due to her status as a stray. However, in the ending scene, her fur is much neater and has a softer look, implying Penny or her mother cleaned her up.
- Platonic Life Partners: Ends up being this with Bolt.
- Slapstick Knows No Gender: Somewhat Downplayed, but occasional mild injuries, and Bolt's abuse of her when he thinks she's an agent of Dr. Calico, are somewhat Played for Laughs.
- Talking Animal: Like every other animal character in the movie, she's one of these of the "can talk to other animals but not to people" variety.
Voiced by: Mark WaltonA hamster that meets Bolt and Mittens.
- Ascended Fanboy: As a fan of Bolt's TV show, he's very excited about getting to join Bolt on an adventure.
- Badass Adorable: For a hamster, and deluded crazy one at that, he's pretty badass.
- Bubble Boy: Some gags reminiscent of this trope, although he can leave his ball when necessary.
- Cloudcuckoolander: Thinks Bolt's TV show is real despite the fact that no-one is tricking him into thinking that and he actually watches it on TV. He refers to the TV as the "magic box".
- Death Bringer The Adorable: Who names a hamster 'Rhino'? ...up until this movie came out, anyway?
- Genre Savvy: Coexisting with Wrong Genre Savvy: that is, his dramatic instincts are correct for the Show Within a Show, incorrect for the 'real' world of Penny and Bolt, but correct again for a character in an 'Incredible Journey'-type movie made by Disney.
- Large Ham
- No Indoor Voice
- Power of Friendship: He completely believes in this trope.
- Ridiculously Cute Critter
- Rousing Speech: For someone living in a delusional fantasy, he's quite good at these. He delivers very hammish speeches that somehow manage to be really good advice despite the fact that his reasoning behind what he says is completely Wrong Genre Savvy.
- Talking Animal
Voiced by: Miley CyrusBolt's beloved owner.
- Badass Adorable: She is a very cute girl. Not so much of a Badass, but she plays one on TV.
- Brown Eyes
- Curtains Match the Window: Her short brown hair and her Brown Eyes.
- The Cutie
- Damsel in Distress: Plays one in the Show Within a Show, then becomes one for real at the climax of the movie.
- Disappeared Dad: Off set. On set, she has a Missing Mom.
- Heroes Love Dogs: Isn't this kind of obvious?
- Protectorate: To Bolt.
- Red Is Heroic
- Tights Under Shorts