Voiced by: John Travolta
- Amplified Animal Aptitude: On and off the set.
- Ass Kicking Pose: Before delivering the Superbark, Bolt always assumes the position. Right forepaw, forward. Right hindpaw, backward. Then, WOOOOF!!!
- Canine Companion
- Cloudcuckoolander: Though this was because the director and production team made Bolt believe the show was real.
- The Comically Serious
- Conspiracy Theorist
- Determinator: He will not give up searching for Penny no matter what.
- Female Feline, Male Mutt: With Mittens.
- Fish out of Water
- Flying Brick: In the TV show he's the star of, anyway.
- Heroic Dog
- Interspecies Friendship: With Mittens and Rhino.
- Large Ham
- Locked into Strangeness: Subverted. He believes the lightning mark in his fur is a symbol of his powers, but it's actually just make up.
- No Social Skills
- Ridiculously Cute Critter: As a puppy.
- Stock Superpowers: Not real, of course.
- Talking Animal: Only other animals can understand him, though; he just sounds like a normal dog to humans.
- Undying Loyalty: Raised from puppyhood to be Penny's protector.
Voiced by: Susie Essman
- Balloon Belly: And she loved every minute of it.
- Broken Bird
- Cats Are Mean: Subverted. Initially, she kicks around the pigeons and makes them bring her food, but during her journey with Bolt and Rhino, she softens up and grows a heart.
- Cats Are Snarkers: She's the page picture, for god's sake.
- Cat Stereotype: She's black and white, so she's snarky.
- Dark and Troubled Past
- Deadpan Snarker
- Defrosting Ice Queen
- Female Feline, Male Mutt: With Bolt.
- Freudian Excuse
- Green Eyes
- Heel-Face Turn
- Interspecies Friendship: With Bolt and Rhino.
- Jerk with a Heart of Gold
- Lean and Mean: At first.
- Only Sane Animal
- The Pigpen: Is rather dirty and scruffy-looking due to her status as a stray. However, in the ending scene, her fur is much neater and has a softer look, implying Penny or her mother cleaned her up.
- Slapstick Knows No Gender
- Talking Animal
Voiced by: Mark Walton
- Ascended Fanboy
- Bubble Boy: Some gags reminiscent of this trope, although he can leave his ball when necessary.
- Cheerful Child
- Death Bringer The Adorable
- Genre Savvy
- The Lancer
- Large Ham
- Neck Snap: Played for laughs when he casually mentions his intent to do this to a guard despite the size difference.
- No Indoor Voice
- Power of Friendship: He completely believes in this trope.
- Ridiculously Cute Critter
- Rousing Speech: He delivers one that snaps Bolt out of his Heroic BSOD.
- Talking Animal
Voiced by: Miley Cyrus
- Brown Eyes
- Curtains Match the Window
- Damsel in Distress: Plays one in the Show Within a Show, then becomes one for real at the climax of the movie.
- Disappeared Dad: Off set. On set, she has a Missing Mom.
- Heroes Love Dogs: Isn't this kind of obvious?
- Protectorate: To Bolt.
- Red Is Heroic
- Tights Under Shorts