* In the series ''Franchise/{{Star Wars|ExpandedUniverse}} ComicBook/KnightsOfTheOldRepublic'', Big Bad Haazen has one of these right before getting annihilated by the combined firepower of an entire fleet of warships.
* In a flashback in the ''ComicBook/{{Thunderbolts}}'' 1997 Annual, Melissa Gold (in her costumed persona of Screaming Mimi, possessing sonic powers) obliterates a portion of forest, liquefies the ground around herself, and destroys her vocal cords upon the death of her boyfriend Angar, by falling to her knees and screaming for 48 minutes straight, until she shreds her vocal cords and can't do it anymore.. She gets better.
* In an issue of the ''ComicBook/SonicTheHedgehog'' comic (#135), this is Sonic's reaction to [[spoiler:the apparent (and brief) death of his father]], represented by him screaming helplessly with a big "N" and "O" to either side. It... doesn't really work. Points for trying, though.
* It happens several times in ''Comicbook/{{Supergirl}}'' story ''Comicbook/TheSupergirlFromKrypton'':
** When Kara and Amazon Artemis duel, and the latter is apparently about to run her sword through Kara, Franchise/{{Superman}} overreacts:
--->'''Superman:''' NO!
** Supergirl shouts one when Comicbook/{{Darkseid}} fires his Omega Beams at Superman.
* In ''Franchise/{{Superman}}'' story ''Comicbook/SupermanBrainiac'', Pa Kent shouts one when he sees a missile heading towards his home and his wife.
* In the original ''[[ComicBook/TheTransformers Transformers Generation 1]]'' comics, Prowl got a classic one in response to the second death of Optimus Prime.
* ''ComicBook/{{Sillage}}'': Nävis, [[spoiler:at the end of the first book]].
* ''Franchise/{{Batman}}'':
** Batman's reaction to the death of Jason Todd in ''ComicBook/ADeathInTheFamily''.
** In ''ComicBook/BatmanYearOne'', Gordon's reaction to [[spoiler:his infant child being dropped from a high bridge.]] Luckily, Batman saves the day.
** ''ComicBook/BatmanTheDarkKnightReturns'': The leader of the "Sons of the Bat" -- Mutant gang members who have flocked to Batman (or their idea of Batman) as a symbol declares the blackout in the city as an ideal opportunity to KillItWithFire. The Dark Knight's refutation is so stentorian that the ''[[PaintingTheFourthWall speech balloon]]'' is in the shape of a Big No.
--> '''[=SotB=] leader:''' ''Gotham City'' is helpless -- this is ''our chance'' to raze Gotham -- to ''purge'' Gotham --\\
'''Batman:''' [[http://img40.imageshack.us/img40/5782/img0016dv.jpg NO]]
* ''ComicBook/TheInhumans'':
** Inverted by {{Black Bolt}}. He may only whisper "no", but the extreme volume of his voice would make the whisper loud enough to blow up a planet.
** He gives a genuine one in ''ComicBook/WarOfKings''. And we see why he usually whispers.
* In an issue of Creator/ValiantComics' ''ComicBook/TheLegendOfZelda'', Zelda points out Link's defeating Ganon is pointless because [[spoiler:"You don't have to join Ganon! You've become Ganon!"]] You can guess Link's response.
* In ''ComicBook/TheDarkness'', Jackie Estacado when he learns that he CantHaveSexEver.
* In ''ComicBook/FinalCrisis'', Overman's reaction to finding out his beloved cousin Kara Kant/Overgirl is dead. Since he's German, he goes "Neinnnnnn!"
* When [[spoiler:Jane Foster is killed (don't worry, she gets better)]] in ''ComicBook/TheMightyThor'' #371, Thor spends an entire page smashing stuff up while shouting [+"NO!"+]. He ends up kneeling in the wreckage, saying one final, very small, [-"No."-]
* ''ComicBook/XMen'':
** In ''Uncanny X-Men'' #300, [[SmugSnake Fabian Cortez]] is informed that he's no longer the top contender among the Upstarts.
--->'''Cortez:''' How could I possibly lose the points for [[spoiler:[[ComicBook/{{Magneto}} Magnus]]]]'s death, unless...? ([[OhCrap his eyes suddenly widen]]) '''NOOO!'''
** In an early issue of Creator/ChrisClaremont's ''X-Men'':
--->'''Omniscient Narrator:''' You and the X-Men have saved the '''world''' from a nuclear holocaust, but you lost a man to do it... and '''try''' as you might, you can't '''balance''' those scales in your mind '''or''' in your heart... '''can''' you, Cyclops?\\
'''Cyclops:''' No.\\
'''Narrator:''' Can you?\\
'''Cyclops:''' No!\\
'''Narrator:''' '''CAN YOU?''' \\
'''Cyclops:''' '''NO!!'''
** In the story "Kill or Cure", from Iceman, when Mystique reacts badly to his final rejection of her (she jumps off the San Francisco Bay Bridge).
* Ryu in [[ComicBook/MalibuComicsStreetFighter Malibu's]] ''Franchise/StreetFighter'' comic gives one [[spoiler: when he finds out Ken's dead]].
* ''Creepy'':
** In "The Haunted Sky" in ''Creepy'' #30 a test pilot yelled one when the ghosts of buddy pilots from the wars he'd served during made him crash his plane.
** In "Lifeboat" in ''Creepy'' #34 the last survivor of a dead race yelled one when he was tossed out an airlock.
* Amelia from ''ComicBook/AmeliaRules'' screams a NO! that fills an entire page after [[spoiler: regretting that she gave up her spot as a cheerleader to her friend Rhonda]].
* ''ComicBook/TheGoldenAge'': Thunderbolt, when his master Johnny Thunder gives him the command to kill Hourman during the Dynaman revelation incident, became so conflicted with that and his own personal ethics that he just departed from the scene entirely, screaming this.
* "Give Up?", a ''[[Franchise/ArchieComics Betty and Veronica]]'' comic, has Betty giving Veronica this when Veronica asks Betty if she'll -- you guessed it -- dump Archie.
* ''ComicBook/NemesisTheWarlock'': Nemesis, near death at that point, gives a big "NOOOO!" when Torquemada reveals his last plan to activate his [[DoomsdayDevice "Final Solution"]].
* ''WesternAnimation/ThePowerpuffGirls'' story "Steal A Meal" (Block Party #26) has the girls giving out with a big tearful "NO!!!" when the only cereal left on grocery shelves is oatmeal. (Him brought three cartoon cereal mascots to life and had them steal all the cereal in town.)
* ComicBook/BrodysGhost has Talia howling out an enraged "NOOOOOO!" when [[spoiler:Brody refuses to choke her murderer to death as she desires, instead planning to leave him for the cops to arrest and convict legally.]]
* ''Comicbook/{{Flashpoint}}'': Deadman gives a big "NO" once he realizes he's now a ghost.