Immediately afterwards, Kuzco-as-a-llama gamely attempting to start a new life as an ordinary llama.
When Kuzco and Pacha use a back to back wall climbing method the second time.
"Bring it on." It's quoted a lot in regards to this movie, but the gravity of the situation tends to be lost... Kuzco and Pacha really have no reason to believe they'll survive that trip over the waterfall.
When they go over the falls, you can hear Kuzco screaming... the word "BOOYAH!"
Pacha forgiving Kuzco in the meadow. When you consider that Kuzco double-crossed him twice (and mocked him about it), it shows incredible moral character. He doesn't even need the apology Kuzco tries to give him.
A minor example is the palace dinner scene, where Kronk manages to gloss over losing track of which drink had the poison in it by mixing all the drinks together. He even manages to remember to warn Yzma not to drink hers. For a big ditzy guy with the attention span of a teaspoon, that's pretty impressive.
Kuzco's concern for letting Pacha leave his family with Yzma and Kronk is quickly proven to be unfounded, as they make quick work of Yzma, with Kronk, naturally, none the wiser, with nary a struggle. Badass Family indeed!