Uncle Ben, of all people, got one. When Charlie Weiderman was hiding in Parker's home, Ben told the jocks who wanted to beat him to go away. Flash Thompson tried to attack him instead. And our old, dear Uncle Ben KICKED HIS ASS!
During J Michael Straczynski's opening arc, Spidey met a dude called Morlun, who said his need to suck the Life Energy out of Spidey was nothing personal. Spidey's response:
"Waitaminnit... you're saying you're gonna kill me and it's nothing personal? Nothing personal? Listen, Buddy, I've fought every kind of nutball on the planet. I've fought freaks, mutants, aliens and high-tech gangs. Heck, I've fought my own costume. And you know what? You're the first one who's really ticked me off. You want me? Bring it on, chowderhead. Bring it on."
At the end of that same arc, having gotten the tar kicked out of him by Morlun, Spidey uses a desperate plan involving radioactive isotopes. Morlun proceeds to become a lot less fearsome. Spidey then delivers a royal beatdown while declaiming:
"How does it feel, Morlun? How does it feel to be facing someone who won't give up and run away? How does it feel to be on the receiving end of the unstoppable force? How. Does. It.FEEL?"
His thought just before injecting himself with radioactive goo:
A little while before that, when Morlun has a beaten, exhausted, one-eyed Peter on the ropes and Ezekiel shows up - a recently acquainted mysterious older man sporting Peter's exact powers:
"This must be what it's like like when you need a hero to save your life and look up and see me."
Pretty much all of issue #500, which featured Spidey reliving his entire career as a super hero up to that point.
To further elaborate, Spidey has been flung back in time due to a weird magic explosion caused by a fight between Dr. Strange and Dormammu. In order to find his way back into the present, Spidey must travel back into the future, and he must relive every exploit he has ever had, one fight at a time. There is so much violence and tragedy, he can barely catch his breath as he teleports from one fight only to start another, and at times he feels it's hopeless and wants to quit, but for the patience and encouragement of Dr. Strange, he fights on, until he makes it to the present...
In which he has to convince the Thor, Iron Man, the Fantastic Four and many other heroes to stall for time instead of closing a dimensional portal which would unleash Dormammu. And they trust him, because of all the battles he had endured throughout his career, he has proven that he is a hero worthy of their respect and trust, even when he suggests something counter-intuitive, and they manage to save the day. The entire issue is a pretty awesome metaphor for Spider-Man as a character and as a comic book, capping off an awesome milestone with...
Uncle Ben, resurrected for 5 minutes, telling Peter that he has lived his life well, and that he's happy for Peter.
In the Book of Ezekiel storyline, Ezekiel has sacrificed Spider-Man to placate a spider god/demon/thing from hunting him, having obtained his powers identical to Spidey's through a forced ritual a long time ago. As the spider demon comes to claim Peter's life, they switch minds/hearts/thoughts, and they relive each other's lives. It goes like this.
Ezekiel: I tried to help but there was nothing I could do—
Ezekiel: Why do I seek the power of the Spider? So that I can help people, without it there is nothing I can do—
Ezekiel: Before I can get started I need a base of operations, a company—
Ezekiel: Look, we can't get anything done unless the full Board of Directors can agree on—
Ezekiel: I know they're in trouble, but I told them not to go, and there's no time right now, I can't—