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Storming Hideout Helm is one all throughout. The music, the timer, and the fact that you have to use all five Kongs to work together to reach the end, as opposed to everyone going their own ways to find their own bananas.
All the kongs defeating their respective bosses, but special mention goes to Tiny Kong for defeating Mad Jack. It's basically a battle between a little kid and a giant, nightmarish living Jack-In-The-Box that takes place high up on several pillars. Tiny faced up against what is often sighted as the most terrifying boss in the history of the DK series and came back victorious.