The first ten minutes of Bolt lampshade the entire idea of a 'Crowning Moment of Awesome'.
Bolt's heroic rescue of Penny from the fire
Rhino gets three— he delivers two heroic speeches, first to Bolt on the importance of heroes, then to Mittens on the importance of sticking with one's friends... then at the climax he uses his hamster ball to prop up the falling rubble of the studio so Bolt can get past, screaming "It is a good day to die!!!" as his ball starts to crack under the weight...
Also doubles as Crowning Moment of Funny since even though Rhino has his arms raised, and is straining with the effort as he "holds the door up" the ball is taking the entire load, and he's just standing in the middle of it.
Mittens comes to rescue him from being crushed. "Not on my watch, rodent!" indeed.
Don't forget the scene in which Rhino, through Crazy Awesome luck, manages to BLOW UP A PICKUP TRUCK!
Penny's mother punching out Mr "lets stick a pin in it" out of the ambulance and quitting the show.
The Superbark. The real Superbark.
Bolt proving that a dog doesn't have to be genetically altered to have amazing abilities.
Mittens runs after Bolt to try to convince him to go home again, telling him that she was wrong and that Penny loves him more than anything. Neither of them realize that the stage has caught on fire, or that Penny is trapped inside. Blocks away, Bolt is silent and looks like he's ignoring Mittens' explanations, and finally this happens:
Mittens: No, Bolt, you need to hear this! She-
Bolt: Mittens, be quiet!
Mittens:*realizing that he's listening intently to something* ...What is it?
Bolt: *staring at her in horror* Penny.
The signature moves from the show? Ten times cooler when used in real life.
Penny, blinded by smoke, stumbling, can no longer fight the effects of smoke inhalation enough to keep up with Bolt on her own. And then she finds a rope.
The last-second rescue, as well.
Penny's character had just minutes ago been calling for the fake Bolt, not expecting him to hear, and the "character" Bolt arrived anyway. Right before Bolt's return, she was coughing and calling helplessly for anyone to find her...and, just like he always has, Bolt came.
Out of universe, Mark Walton, the voice of Rhino, was a studio employee tapped to record the test track for Rhino, with the expectations that his part would be overdubbed once they found the "real" voice actor. The directors were so impressed with his performance that they decided to keep him as the voice of Rhino. His overwhelmed, excited reaction on the DVD extras is awesome, hilarious and heartwarming.