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When Tsukune and friends spend New Year's Day at home, an offscreen voice says it's because crowd scenes are hard to animate.
When Tony comments that Tsukune has changed a lot since he last saw her, he pulls out a volume of the Tsukune-chan manga for reference. Kokoro then remarks that the anime’s character designer used recent volumes of the manga for reference, and Mr. Mayor asks “Can we talk about something like that?”
When Shioretta shows up in her Yamato Nadeshiko garb, Tsukune takes a moment to recognize her and then asks "So you aren't a new character?”
Non-Human Sidekick: Pupurin is this... for about the first 50 seconds of the first episode, at which point Tsukune casually roasts him alive to feeding a starving man. Afterwards, his ghost fills this role.
Ton-chan is Kokoro's cute piggy sidekick... who likewise only lasts about a minute before one of Kokoro's explosive sneezes turns him into a pork roast.
Offscreen Moment of Awesome: There's an episode called "Tsukune-chan vs. Aliens." It's five seconds long, and all we see is the mayor asking Tsukune-chan for help, and then a brief shot of the alien ship blowing up. Afterwards, an off-screen voice says "But I wanted to see that..."
Why Don't Ya Just Shoot Him?: After using a bunch of spells to try and beat Nabule, Tsukune finally brains her with a nearby pot, commenting “I should’ve just done that right from the start instead of using magic.”