* ''Don't Fear The Dark'': Lieutenant Starkey.
* ''WebVideo/TheFantasticFavioBros'': [[TheLancer Tony]] often acts as the foil to [[TheHero Favio]]. As such, while Favio is a ChivalrousPervert, Tony is ambiguously gay. His EvilTwin, [=LeTony=], also has traces of ambiguous sexuality.
* Nick and Beck of Theatre of Life fame. [[http://www.youtube.com/watch?v=KgY_rNQj-EU They do it on purpose.]]
* Coach Z of ''WebAnimation/HomestarRunner'' was shown married to Bubs and has worn a skirt and hair curlers in two different toons. The only reason why he is ambiguous is because his sexuality is never mentioned. He's likely AmbiguouslyBi due to his obsessive attraction to Marzipan.
* Barry 'The Blender' Henderson and Thomas 'The Tanker' Smythe of ''I Am Fighter'' both fit this trope to some extent; Thomas frequently takes it to Squick levels.
* WebVideo/LookAVlog: Will.
* ''Machinima/RedVsBlue'': Donut. In Season 1, he had been given pink armor ("It's not pink, it's ''lightish red''!") and was your typical new recruit. In Season 2 (where this trope comes into play) he started using lotions, talking about his feelings, screaming like a woman, analysing dreams, and generally being as effeminate as possible while retaining some air of heterosexuality. It's gone by Season 3.
-->'''Tucker:''' I don't think Santa's outfit is a biker's costume and a codpiece, Donut.\\
'''Donut:''' It was the best Christmas ever!
** The song "Donut: The Musical" from the Season 9 soundtrack plays it straight. The song is full double-entendres and similar sounding words (that the other Red members keep asking to repeat). And he still thinks he can get some "elbow grease" and "headlight fluid".
--> "Let me blow you... away"
* In ''WebVideo/YuGiOhTheAbridgedSeries'', Pegasus is an even stronger example of this trope than the original. He even goes so far as to violently defend this image, as once the side characters find out that Pegasus had a wife, he knocks them unconscious with the words "I can't have people thinking I'm straight".
** Bakura, to the point where his ''own personalities'' can't agree on the issue.
-->'''Bakura:''' I'm not gay, I'm just British!\\
'''Yami Bakura:''' I'm not British, I'm just gay.
** Marik is either in a TransparentCloset or out and dating Yami Bakura, although it's less ambiguous and more Marik failing to even realise he's gay.
-->'''Marik:''' Oh, what, just because a guy likes to dress effeminately and hang around with another extremely attractive man and read yaoi and flaunt his gorgeous abs and stroke a phallic symbol suggestively in every other scene, that automatically makes him gay?!\\
'''Bakura:''' ...Kinda.
* ''WebVideo/DragonBallAbridged'' plays Zarbon's ambiguous sexuality for as many jokes as it can. Soon before his death however it is revealed, to the surprise of '''everyone''', that [[spoiler:he is completely straight]].
* Jean-Armand (codename: Nephandus) of the Literature/WhateleyUniverse. Obsessed with his looks and his hair and his clothes. Chaka even refers to his standard outfits as a 'Merchant-Ivory wardrobe'. A mama's boy, although his mother is a notorious European supervillain called the Troll Bride. Carries a walking stick (although it's a weapon too). Even his friends in the Bad Seeds make jokes about his ambiguity.
* The hosts of ''WebAnimation/{{Spill}}'', in their review of ''Film/BeautifulCreatures'', claim that [[SouthernGentleman upper-class, "posh" Southern accents]] come off as sounding like this.
* [[http://www.fictionpress.com/s/3083396/20/Psychronicles Chapter 21 of Psychronicles]]. Is it so hard to guess who?
* Omega Zell from ''Series/{{Noob}}'' is a reporter for a feminine channel in real life, cares for his avatar's appearence over anything else, is frequently forced into an InvoluntaryDance and acts as if he has a crush on the game's (male) top player. His YaoiFangirl guildmate speculated he could be gay from day 1, but he has always denied it. Season 4 has him reveal that he's deliberately avoiding relationships with women to be able to focus on his dream of becoming the next top player of the game (that and [[EffeminateMisogynisticGuy he sort of hates them]]). However, neither the series creator nor the fans seem able to stop making jokes about him having a crush on any male character whose actor ends up in close proximity to him on "behind the scenes" photos.