* DesignatedMonkey: Mr. Bump is becoming one.
* EarWorm: Quite a lot of the songs, especially ThatOneSong that gets stuck in your head.
* FridgeBrilliance: In "Flying", Mr. Grumpy says that he started working as a flight attendant 30 years ago. 30 years before the episode premiered was 1978, when Mr. Grumpy's book was released.
* HilariousInHindsight: When the show first came out, various fans of both this and [[WesternAnimation/ThomastheTankEngine Thomas & Friends]] did parody clips depicting several Thomas characters as the Mr. Men and Little Misses. A year later, some of the UK voices from the show would play characters for Thomas.
* MemeticMolester: Mr. Tickle comes as close as a character can for a lighthearted kids' show.
* SeasonalRot: The second season could be considered weaker than the first.
* StopHelpingMe: Little Miss Helpful isn't always help, especially to ButtMonkey characters.
* {{Squick}}: Mr. Messy has some rather revolting habits, especially to a NeatFreak like Mr. Persnickety/Fussy.
* TheyWastedAPerfectlyGoodCharacter: Mr. Tall and Little Miss Curious. For most of Season 2, they were relegated to nothing more than cameos, and even their speaking appearances had little to no bearing on the plots of the sketches they appeared in. Mr. Tall would've had great plots concerning his height, and Little Miss Curious could start one off with a question. Alas, it was not to be.
* TheyWastedAPerfectlyGoodPlot
* ToyShip: Despite the series aimed for young children, at one point, it seems, during Season 2, that the writers and animators were teasing of hints of Mr. Grumpy with Miss Chatterbox for some odd reason.
* UnfortunateImplications: Mr. Tickle
** It can't be the only one who's noticed Mr. Rude has a French accent.
*** And Mr. Stubborn has an African accent.
* ViewerGenderConfusion: Little Miss Scary.
* TheWoobie: Mr Bump. He has Miss Whoops for a sister. I'd hate to be him. And in some cases, this trope can expand to Mr Quiet. Miss Calamity, until her disappearance in season 2, was also part of this trope.
** However, since some people are amused by how Mr. Bump gets repeatedly harmed, you could describe him as TheChewToy. Miss Calamity fitted this trope, too.
** Mr. Happy is also considered one. There are some fans who feel sorry for him. While the Chew Toys/Butt Monkeys always express how they feel and get back on their feet, Mr. Happy has to hide his other emotions behind a smile and suffer in silence.
** Mr. Nervous. He's practically scared of life itself!
** Mr. Quiet - the poor dude is just looking for some peace and quiet but ends up being walked over by others or dragged into their mess.
** Miss Sunshine in the ''Surprise'' skit (even if it was for a moment).