* EarWorm: The opening theme has been known to induce random audience head-bobbing.
* HesJustHiding: Before the release of ''[[Film/ResidentEvilRetribution Resident Evil: Retribution]]'' fans speculated that [[spoiler:Wesker survived the [[CarBomb helicopter mini-nuke]] at the ending of the movie. This is supported by some eagle-eyed viewers noticing a parachute opening in the distance when one of the characters revealed himself to have survived. Wesker's survival was later confirmed by a press release for ''Resident Evil: Retribution''.]]
* {{Narm}}: [[spoiler: Wesker must INGEST fresh DNA.]]
** Although not really, that was just a fancy way of saying cannibalism, and he is seeing eating, and it's not just DNA...
** The T-Virus being shaped like actual Ts...
** [[ThisIsForEmphasisBitch Star power bitches!]]
*** The last one is a huge case of YMMV; many viewers thought it was [[NarmCharm both funny and awesome]].
* TakeThatScrappy: Alice gets brought down to normal, ends up on the receiving end of a CurbStompBattle and has all her clones wiped out. For many, it was considered to be the best thing that the directors did to try and redeem the films. But since Alice still is the only one to do anything in this film it's kind of a moot point.
* TheyWastedAPerfectlyGoodPlot: The previous film has it to where Alice stumbles upon an entire storage unit of her clones and pretty much makes an army. [[TrailersAlwaysLie The build up to this movie was pretty much based around the fact there was a freaken army of super powered women taking on Umbrella]]. The first five minutes into Afterlife as they takes down the Umbrella Base in Tokyo, all of them are killed after the [[SelfDestructMechanism base self destructs]]. A lot of fans looking forward to this were obviously ticked off at this.
** And if that wasn't enough, after spending pretty much the entirety of the last Film developing Alice's powers, she is BroughtDownToNormal just after her army is wiped out
*** Though a lot of fans saw the death of [[BlackHoleSue Alice's]] clones and her being BroughtDownToNormal as an epic TakeThatScrappy