* EarWorm: The opening theme has been known to induce random audience head-bobbing.
* HesJustHiding: Before the release of ''[[Film/ResidentEvilRetribution Resident Evil: Retribution]]'' fans speculated that [[spoiler:Wesker survived the [[CarBomb helicopter mini-nuke]] at the ending of the movie. This is supported by some eagle-eyed viewers noticing a parachute opening in the distance when one of the characters revealed himself to have survived. Wesker's survival was later confirmed by a press release for ''Resident Evil: Retribution''.]]
* {{Narm}}: [[spoiler: Wesker must INGEST fresh DNA.]]
** Although not really, that was just a fancy way of saying cannibalism, and he is seeing eating, and it's not just DNA...
** The T-Virus being shaped like actual Ts...
** [[ThisIsForEmphasisBitch Star power bitches!]]
*** The last one is a huge case of YMMV; many viewers thought it was [[NarmCharm both funny and awesome]].
* TakeThatScrappy: Alice gets brought down to normal, ends up on the receiving end of a CurbStompBattle and has all her clones wiped out. For many, it was considered to be the best thing that the directors did to try and redeem the films.
* TheyWastedAPerfectlyGoodPlot: The previous film has it to where Alice stumbles upon an entire storage unit of her clones and pretty much makes an army. [[TrailersAlwaysLie The build up to this movie was pretty much based around the fact there was a freaken army of super powered women taking on Umbrella]]. The first five minutes into Afterlife as they takes down the Umbrella Base in Tokyo, all of them are killed after the [[SelfDestructMechanism base self destructs]]. A lot of fans looking forward to this were obviously ticked off at this.
** And if that wasn't enough, after spending pretty much the entirety of the last Film developing Alice's powers, she is BroughtDownToNormal just after her army is wiped out
*** Though a lot of fans saw the death of [[BlackHoleSue Alice's]] clones and her being BroughtDownToNormal as an epic TakeThatScrappy