* BigLippedAlligatorMoment:
** Episode 28.5, "The Last Episode Ever". It first aired on April Fools Day.
** Episode 45.9, "The Greatest Episode EVER". An episode made entirely with paper cutouts by Gus Sorola while the rest of Rooster Teeth was out of town.
** ''Out of Mind'': Part 2's flashback segment comes off as this retroactively, seeing how it shows Texas and Alpha-Church talking casually while it's shown the Blues and the Project were capable of actively interacting with one another. It's later shown that this whole flashback was ''very'' incorrect in many ways, and it's never remotely explained why Texas' memory would be so wrong.
* CrossesTheLineTwice: Much of the series' humor, though special mention probably has to be given to Private Jimmy's absolutely ''hilarious'' CruelAndUnusualDeath.
* EnsembleDarkhorse: Viewers of ''Out of Mind'' loved Delta and York. And both Sister and Junior were very popular despite only having starring roles in Season 5.
* FranchiseOriginalSin: In the beginning, the show was always about laughs and invoking the RuleOfFunny. Antics like a robot head that could built other robots, time travel, ghosts and being transported into what was all but stated to be an actual game of ''Halo'' were commonplace and accepted due to the quality of the jokes. And even when the series moved into more serious fare, the show was good about retconning or sidelining those as needed while doling it out just right to ease the tension. It only truly became more of an issue in Season 16, which went whole hog on being funny by suddenly introducing gods, real time travel and a cyclops done in live-action. Such a sudden veer back into territory even more outrageous than the first seasons had done wound up coming across as too jarring and unexpected.
* GrowingTheBeard: It was during Season 3 that the series slowly began to head towards a plot-driven structure with some moments of genuine CharacterDevelopment. This was dialed down a bit in the fourth season, but soon returned in the middle and would stay that way for the rest of the arc's run.
* HarsherInHindsight:
** After learning of [[spoiler:Private Jimmy's]] ''true'' fate in Season 14, Tucker and Church's conversation about him in "A Shadow of His Former Self" is subtly horrifying.
** Sarge's hilarious BloodKnight tendencies, InsaneTrollLogic, and habit of shooting/trying to kill [[ButtMonkey poor Grif]] are all rather uncomfortable to watch as not only would Season 14 display [[spoiler:him as being so crazy that he ''killed his own [=C.O.=]'' when being picked out as the Blood Gulch Red Team's sergeant since he thought he was a Blue in disguise]], but later seasons (most notably ''Revelation'', Season 15, and ''Singularity'') would start to take a more critical and tragic view on how much of a negative impact being in the military for so long has had on Sarge's psyche.
** Captain Butch Flowers dying of an allergic reaction to some medication is this twofold; the first reason is that we find out [[spoiler:this was almost certainly Gamma torturing Church ForTheEvulz]] in Season 10, and the second is due to Creator/{{Monty Oum}}'s tragic death from a severe allergic reaction after surgery in 2015.
*** ''Most'' things about Captain Flowers become this in hindsight after Seasons 10 and 14 [[spoiler:reveal that he's actually Freelancer Agent Florida, a ruthless [[TheSociopath sociopath]] who barely batted an eyelash as Sarge killed his subordinate Lemons (even cheerily [[YouHaveOutlivedYourUsefulness thanking him for finding the perfect candidate]] as Lemons pleads for help over the comm), and [[UnwillingRoboticization forcefully implanted]] the Alpha A.I. into Private Jimmy, completely shattering the image of AFatherToHisMen he had up until then]].
** When the bomb inside Church is about to be activated, Tucker attempts to kill him [[TheNeedsOfTheMany in hopes of destroying the bomb]]. [[ItsAllAboutMe Church objects to that]], [[LackOfEmpathy not caring about the people around him in the slightest]]. [[spoiler:[[CharacterDevelopment This attitude comes to a change later]], as performing a HeroicSacrifice with an uncertain hope of success is exactly how Church meets his end ''[[WebAnimation/RedVsBlueTheRecollection twice]] [[WebAnimation/RedVsBlueTheChorusTrilogy over]]'']].
** Tex not showing any hesitation to kill Grif when asked to, once [[WebAnimation/RedVsBlueTheShisnoTrilogy Season 15]] reveals that [[spoiler:it wouldn't be the first time she horrifically killed a simulation trooper in orange armor with no remorse (though her using [[UnstoppableRage Omega]] at the time then admittedly complicates the situation)]].
** While threatening Andy, Tex emphasizes how he will be just a memory after she detonates him. The amount of irony is '''staggering''' [[spoiler:seeing what becomes of Tex after both ''Reconstruction'' and ''Revelation'']].
** Caboose's cheerful shout of "Connecticut!" is this when Tex and Wyoming meet each other again, now that the audience knows that [[spoiler:Connie was one of the few Freelancers Tex had any kind of emotional bond over. Furthermore, Connie's death had the greatest impact on her out of anyone and helped [=kick-start=] her HeelFaceTurn]].
** Most of the second half of Season 4 and all of Season 5 portrays Church as on the verge of reaching his RageBreakingPoint as he struggles to keep Blue Team at least ''partly'' functional despite the world seeming to fall apart around them. This is largely [[PlayedForLaughs Played For]] BlackComedy at the time, but ''The Project Freelancer Saga'' would later reveal that [[spoiler:the Alpha [=A.I.=] was successfully tortured past the brink of insanity multiple times by having him be ForcedToWatch as the people he cared about most died horribly and he was told that it was [[ItsAllMyFault all his fault]]. So in essence, Church is pretty much going through his worst possible nightmare all over again throughout the last portion of ''The Blood Gulch Chronicles''... and he ''doesn't even realize it''.]]
** When Grif's Sister appears in Season 5, her having run away from home to be with him in the ''military'' is PlayedForLaughs and {{Hand Wave}}d away at the time as the result of her being TheDitz. Not only would later seasons take a more serious look back at the Grif family (with Season 13 claiming that Grif was TheUnfavorite and he was PromotedToParent due to the siblings' [[AbusiveParents parents being utterly despicable]]), but that (as revealed in ''Singularity'') [[spoiler:Sister actually ran away from home out of guilt for accidentally burning down her and Grif's childhood home.]]
** While Omega's absurd plan to conquer the universe with Wyoming and Gamma's help on the surface can at least be laughed off as the result of the two's manipulations on their own, later seasons would imply that [[spoiler:they were still working for Freelancer Command all along (with Tex/Allison instead being the RogueAgent) and this was actually Project Freelancer's ''best plan'' to stop the Great War]].
** Church discussing battle tactics and strategy with Captain Flowers during the Season 3 flashbacks is a lot sadder after Season 9 would show that [[spoiler:the Alpha was used to help plan out the Sarcophagus heist that would ultimately result in its horrific ColdBloodedTorture. In essence, the Alpha overlooking battle plans is the exact thing that got him stuck in Blood Gulch in the first place]].
** During Season 3 and when Church is traveling through time, he meets up with his past self from Season 1 when he's going through his first death, with [=Future!Church=] awkwardly telling his past self that "You should rest now." This is ''really'' hard to watch now after [[spoiler:Epsilon-Church {{Mercy Kill}}s Tex in Season 9 after she makes it clear that she's tired of being brought BackFromTheDead only to end up as a CosmicPlaything and by this point just... well, [[DeathSeeker "wants to rest."]]]]
*** The time loop shenanigans in the aforementioned season also have Church giving a heartfelt apology to Tex for failing to be a better person to her than he should have been, which is pretty depressing to see after later seasons show [[spoiler:how messed up their romance really is and how this can be seen as the Alpha subconsciously trying to apologize to Beta for him having doomed her to always fail at the last second]].
*** Hell, pretty much ''all'' of Church and Tex's SlapSlapKiss dynamic becomes this once later seasons show how they're actually both [[spoiler:LovingAShadow, as their entire relationship is only a reflection of the Director and Allison Church's romance]].
** In "Biting the Hand", Tex briefly recounts the events of the ''Out of Mind'' miniseries to Church, with the latter asking if Carolina was with York when she met up with the latter. While it's just a minor detail at the time, it's rather sad to see after ''The Project Freelancer Saga'' would reveal that [[spoiler:Church can't remember almost anything from his time as the Alpha [=A.I.=]. That means one of the only things he still remembered from that period was the daughter of the man he's based on]].
** Tex gently whispering "Goodbye" to Church as she lifts off on the Pelican in "Why Were We Here?" is pretty tragic to watch after ''The Project Freelancer Saga'' would delve into [[spoiler:how [[TragicVillain the Director]] (and, to a lesser extent, Carolina) never truly got over the death of Allison Church because they NeverGotToSayGoodbye]].
** Church famously [[HatesEveryoneEqually hates everyone]] on his squad (not to mention everyone ''not'' on his squad). [[JerkassHasAPoint To be fair]], the people he has to work with aren't what anyone would call class-A soldiers, and Church usually has to [[OnlySaneMan play the role of babysitter]]. Still, this was one of the most charming aspects of his character. Then comes Season 10, where late into the season [[spoiler:the Blood Gulch Crew refuses to help him and Carolina track down the Director, Church ([[BackFromTheDead now Epsilon]]) [[TheReasonYouSuckSpeech tears into all of them, telling them that they've been a pain in his side for his whole life]]]]. It is ''significantly'' less funny there.
** In Season 1, Grif saves Sarge from a bullet wound to the head with CPR, and Sarge ([[ItMakesSenseInContext unfairly]]) chews him out for such a stupid choice of medical attention. Years later, when mass shootings became a major hot button issue in 2018, Rick Santorum got into hot water for suggesting students learn CPR.
* HilariousInHindsight:
** The third episode has Grif (voiced by Geoff Ramsey) poking fun at a NoodleIncident GilliganCut involving Simmons (voiced by Gus Sorola) refusing to go to the Vegas Quadrant. One of the ''Creator/RoosterTeeth'' podcasts [[https://www.youtube.com/watch?v=Emdfc_6SQ1Y revealed that this was]] BasedOnATrueStory.
** In the Season 2 DVD commentary, Burnie Burns noted during the "dongle flip" scene that they promised themselves never to do teabagging... only to use the crouch motion in that suggestive way. During the next season there ''is'' actual teabagging, and it's exactly to mock the sort of player who does that (something the DVD commentary even makes sure to lampshade).
** A panicked Grif begging Tex to not hit him in the face once he insults her is ''much'' funnier to watch after later seasons have [[spoiler:all iterations of Tex focus almost solely on giving {{Groin Attack}}s to the poor guy]]. BeCarefulWhatYouWishFor, Grif!
** Caboose claiming that Church is a "gay robot" after learning of Tex and Church's relationship in Season 1 is absolutely ''hysterical'' after Season 14's "Get Bent" would reveal that [[spoiler:Epsilon-Church was perfectly fine [[IfItsYouItsOkay being with]] a [[GenderFlip male version of]] Tex while stuck in the Memory Unit]].
** One of Church's awkward attempts at a PreMortemOneLiner in Season 2's "An Audience of Dumb" becomes this after TheReveal at the end of ''Reconstruction'', considering that this scene has [[spoiler:him partnered up with Tex to take down O'Malley.]]
--->'''Church:''' [[spoiler:[[NotHyperbole I've got half a mind to kill you, and the other half agrees!]]]]
** Also, Church's exasperated cry of "Holy crap, ''who is running this army?!"'' in Season 1 becomes utterly hilarious after the audience finds out in the season finale of ''Reconstruction'' that [[spoiler:''[[YouAreWhatYouHate Leonard Church]]'' is the person actually running the Red and Blue Armies!]]
** When performing a hostage exchange near the end of Season 2, Caboose calls Donut [[ItMakesSenseInContext "Major Cinnamon Bun."]] Keep in mind that Season 2 of ''Red vs. Blue'' aired three years '''before''' the first ''WesternAnimation/AdventureTime'' short even aired.
** Caboose's statement of how "time is made of circles" is pretty hilariously prescient after ''Singularity'' would reveal that [[spoiler:black holes in the [=RvB=] universe loop backward in time to before the Big Bang]].
** In Season 4 when the Blues are hunting for the Alien, Tex sarcastically wonders aloud that if she kills Church while he's a ghost than if that means he would "come back as a ghost of a ghost." [[spoiler:That's essentially what happens in ''The Recollection'': Church is killed in the finale of ''Reconstruction'', and is revived in ''Recreation'' via Epsilon and Caboose's memories of him.]]
** Sheila's appreciation for her new Pelican-class dropship body is quite amusing when it turns out she's almost certainly based off of the Freelancer Dumb [=A.I.=] [[SpaceshipGirl F.I.L.S.S]].
** "No, another ship, then that ship left and this ship crashed in the exact same spot. " Come Season 17, and the revisiting of this moment has something very reminiscent of this sarcastic answer: Sister's Pelican doesn't land on Donut, only for another one to appear and crush him.
** In 2015, the newest line of [[AwesomePersonnelCarrier armored infantry fighting vehicles]] created and used by [[UsefulNotes/WeAreNotTheWehrmacht the Bundeswehr]] were named "[[https://www.bundeswehr.de/en/organization/army/news/the-puma-armoured-infantry-fighting-vehicle-5227994 the Puma AFIV]]". Evidently, someone high-up in the German military might be a fan of ''Red vs. Blue''.
** In the first alternate ending for Episode 100, all of the Reds and Blues end up in a massive free-for-all that ultimately ends with a TotalPartyKill, with Caboose being the last character left alive (at least until a vehicle falls on him). ''Many'' years later in 2020, ''WebAnimation/DeathBattle'' made an episode showing which team would have won if every character had fought seriously like in Episode 100's alternate ending... [[spoiler:and it ''also'' ended with Caboose as the last one alive, except this time he survives the whole thing]].
* JerkassWoobie: As noted below, [[DarkActionGirl Tex]]. [[{{Jerkass}} Church]] also starts to fall into this once it's shown how he's a LoveMartyr that [[ShaggyDogStory completely fails to save his girlfriend from the hands of an evil [=A.I.=] despite his best efforts]] and is full of immense [=self-loathing=] issues.
* MagnificentBastard: [[CombatPragmatist Agent Wyoming]], real name Reginald, and his AI partner [[AIIsACrapshoot Gamma]], representing the Alpha's deceit, are the primary operators of [[BigBad Omega's]] plan. Hired by Omega to assassinate Tucker, Wyoming allowed a bomb to go off that knocked the Reds and Blues unconscious. Gamma manipulated an alien race into a believing prophecy, leading one alien to impregnate Tucker before being killed by Wyoming. After Wyoming sent Omega back to Blood Gulch to find a new host, he confronted and [[spoiler:killed Agent York]], and managed to escape Texas. Wyoming and Gamma reunited and headed to Blood Gulch to retrieve Tucker's alien son for Omega to possess and control the Covenant. Wyoming used his time distortion unit to cheat in battle, and when seemingly killed unleashed time copies to continue to fight. Wyoming's final push [[spoiler:before his death]] was to reveal the full extent of his plan to get Tex on his side.
* NeverLiveItDown: Caboose really only killed Church once with a tank (and that wasn't even entirely his fault). He sort of killed Church while the latter was possessing Sarge, but as a ghost form, he couldn't be killed, and Sarge got better. Yet they won't stop giving Caboose crap about killing his own men. Though granted, this may be because [[RunningGag this won't be the last time that this happens]].
* OneSceneWonder: Private Jimmy, who only appears in Church's flashback in Episode 10 and is used for a hilarious DeathAsComedy joke. He was even brought back for the Season 14 {{Origins Episode}}s, though his reappearance goes through a significant CerebusRetcon.
* RescuedFromTheScrappyHeap: Downplayed with Caboose. When he started out, he was just a slightly slow DeadpanSnarker with a rather flat affect that didn't make him very memorable. However, after the writers [[{{Flanderization}} Flanderized]] him into becoming a {{Cloudcuckoolander}} [[TheDitz Ditz]], he became easily the most popular character out of the entire series.
* RoboShip: PlayedForLaughs in Episode 28.5:
-->'''Doc:''' Everyone! I have something to say! Tucker and I are in love, and we're leaving!\\
'''Tucker:''' That's right! We're getting married and there's not a damn thing you can do about it! Not even ''you'', French-Speaking Lopez!\\
'''Lopez:''' [Mister Tucker, you promised your love to me!]
* TheScrappy: Not many fans like Andy the Bomb, since he had no character traits or depth outside of being a massive (not to mention unfunny) {{Jerkass}}. It's pretty telling that he hasn't made any appearances in recent seasons outside of recordings and has been mostly forgotten.
* SeasonalRot: While Season 3 had received some praise for its more plot driven structure, it was also criticized for downplaying the sharp dialogue and character interplay that made the first two seasons so enjoyable. When Season 4 returned to the show's original formula, many fans were pleased.
* StoicWoobie: Later seasons reveal that Tex had been one of these the whole time. [[spoiler:She knew '''exactly''' the kind of torture that Church had been through, and was simply trying to watch over and protect the broken remnants of someone she had loved (to the point where she had ''demolished an entire military base/spaceship'' trying to save him)]].
** Overlaps with JerkassWoobie to an extent when you realize the reason ''why'' she acts like such a {{Jerkass}} to Church: [[spoiler:Every time she sees him, she's reminded of her failure and that the Alpha will never be what it once was]]. No wonder she can barely stand to be around him, especially after [[spoiler:the Alpha TookALevelInJerkass following the attempted breakout]]. Could also be a façade since [[spoiler:she likely acted so cold to him in order to keep him distant or from becoming too attached to her, since it could -- and did -- lead to him discovering what he really was]].
* TheyWastedAPerfectlyGoodCharacter: Grif's Sister (whose first name, Kaikaina, isn't even ''mentioned'' in the show itself until Season 15) has barely any effect on the story, and disappears almost entirely after Season 5, despite all the fun gags or plot complications that could be built off Grif having a sibling on the other side.
%% ZCE * UglyCute: Junior.
* ValuesResonance: [[ItMakesSenseInContext In a weird sense]]. There's something rather remarkable about ''The Blood Gulch Chronicles'' giving such a dim view on militarization and war for an American web series that only started to air less than two years after UsefulNotes/TheWarOnTerror started.
** The show is actually remarkably popular with American veterans for its depictions of the frustratingly inane and bureaucratic aspects of the military.
* TheWoobie: Both [[TheCutie Donut]] and [[CloudCuckoolander Caboose]]. Sure, they both might be as [[TheDitz dumb as posts]], but they're both still just so adorable and sweet, which makes it all the sadder when things go horribly wrong for them.
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