* AwesomeMusic: Considering that the soundtrack is entirely taken from famous ballet/classical pieces, ''all'' the music in this show might qualify.
** Also, it's produced by Kaoru Wada, well known for the music of anime such as Inuyasha. Doesn't that just scream awesome?
*** Particular candidates for Crowning Music of Awesome are the "Waltz of the Flowers", which plays during Ahiru's first transformation into Princess Tutu in the beginning of the series and her final dance at the end of the series, the "Swan Lake Waltz", which plays during Ahiru and Rue's duel at the end of the first season, and setting [[spoiler:the Demon Raven's revival]] to Danse Macabre.
** Not in the show itself, but the award-winning [[http://www.youtube.com/watch?v=tHZqxecCukg Hold Me Now AMV]] deserves mention for helping to spark interest in the show.
* BigLippedAlligatorMoment: A subversion; Femio actually ''resists'' Kraehe's attempt to take his heart in hilariously preposterous fashion. The subversion is the fact that [[spoiler:the Raven and Mytho actually use this incident as evidence that no one will ever fall in love with the poor girl]].
* DieForOurShip: Rue. '''Oh, Rue'''.
* EveryoneIsJesusInPurgatory : Particularly the short stories at the beginning of each episode and their meaning towards the plot.
* FanPreferredCouple: Very few fans actually pair Ahiru with Mytho, instead preferring to pair her with Fakir.
* FoeYay: Ahiru and Rue's relationship as rivals, and [[spoiler:Princess Tutu and Princess Kaehe's relationship as enemies, at least on Princess Tutu's side]].
* FunnyAneurysmMoment: Episode 7 has a funny conversation between Drosselmeyer and Duck as his attempts to get her to reconsider throwing away the pendant backfire. Upon rewatch it becomes much more chilling, as this is the first real glimpse of how much Duck will suffer later on, and the first indication of how much Drosselmeyer loves tragedies.
* GeniusBonus: Rivals GhostInTheShell for the sheer number of these. Naturally, these are all related to music, dance, and so on, but most people would have to see the translator's notes to get half of these. Big example of ShownTheirWork.
* HoYayShipping: You ''will'' get an "abusive boyfriend" vibe from Fakir toward Mytho; and depending on who you ask, there's also subtext between Ahiru and Rue, as well as Autor and Fakir.
** [[http://www.youtube.com/watch?v=V3ntFSG-Y2k Outtakes]] [[http://www.youtube.com/watch?v=jL403RAEoJE from]] the dub have the voice actors [[LargeHam hamming up]] the HoYay to the max...
** An in-universe example occurs in episode 17, where a raven blood-addled Mytho temporarily regains control and performs the mime for love...in front of Femio. A good few hearts are broken by this "revelation".
** Another in-universe example: When Fakir returns to school after being suspended, Mytho gives him a hug. Cue a roomful of girls screaming with delight. One even faints.
** Fakir acts like a very possessive, abusive lover to Mytho. [[spoiler: When you find out the reason is because Mytho has no self-preservation instinct in his heartless state, it makes more sense and turns the HoYay up a notch.]] Also, during the promotional video, Fakir and Mytho are seen dancing together, and Fakir embraces him from behind. [[spoiler: And upon his suspension ending and Fakir returning to school, Evil!Mytho gives him a great big hug in front of the entire class, which they definitely take notice of.]]
** Ahiru and Rue get some LesYay as well, due to their status as [[FoeYay rivals]] [[spoiler: and, in their heroic forms, enemies]] Not to mention Rue picking Ahiru as her dance partner in episode 2. [[spoiler: And telling each other "I love you" in the final episode. Not as romantic in context, but still!]]
* JerkassWoobie: Kraehe, before becoming a flat-out Woobie.
* MemeticMolester: If he's not already, Drosselmeyer ''should'' be one. Children who like stories, come here!
* MemeticMutation: Ninjas duelling with guitars
** To be more elaborate, the ever-so-epic Guitar Ninjas came into being when somebody posted [[http://img.photobucket.com/albums/v345/Mizuiro/1196486200150.jpg this]] image review of the series. No actual guitar-playing ninjas appear in the series, but [[ImageBoards Anonymous]] agrees that it is still a very apt metaphor.
** Courtesy of the Website/DesuDesBrigade, "Real Men Watch ''Princess Tutu''!"
* {{Narm}}: In Akt 7, Ahiru has a nightmare. It involves Princess Tutu dancing towards Mytho on her tiptoes, and him screaming in fear. Uh...
** In the first episode, Duck's declaration of, "I am Princess Tutu!"
** Mytho's half-hearted screams in the first handful of episodes of the dub. Granted, [[EmotionlessGirl he has no emotions]], but it's still funnier than it is dramatic.
* PeripheryDemographic: At the end of the day, it is a kids' show, but its Gaiman-esque dark fantasy elements can attract viewers of all ages, including older men.
* TheScrappy: Pique and Lilie can certainly wear on most viewers' patience, especially Lilie and her cutesy/sadistic attitudes.
* TheyWastedAPerfectlyGoodPlot: How interesting would it have been if [[spoiler: Rue/Kraehe was REALLY the raven from the legend, as it was implied in the first half of the show before the actual raven appeared? The romantic angle would've been a neat twist.]]
** Also the Book Men, we get so little information about them. Did any other people with the story power manifest? Have they been called into action before? How were they founded? Have they had to stop any other stories from coming to life?
* WhatDoYouMeanItsForKids
* TheWoobie: InUniverse, Ahiru's friends Pique and Lilie view her as this in the story itself, and particularly Lilie seems to really embrace the disturbing hurt/comfort element to the role.
** As mentioned above, Kraehe crosses from JerkassWoobie into this by the end of the series.