* BigLippedAlligatorMoment: Large Marge's face turning into a ghoulish image. [[spoiler: This is because as she had died in a car crash and it was her ghost who picked up Pee Wee.]]
** It's a ShoutOut to the old song "Phantom 309", which features a ghostly trucker named Big Joe.
* CultClassic
* HilariousInHindsight: Pee-Wee driving by the set that's making a ''Franchise/{{Godzilla}}'' film is even more amusing now that Warner Bros actually is [[Film/{{Godzilla 2014}} making a Godzilla film]].
** Also, when Pee-Wee enters the Warner Bros. studio lot for the first time, the Batmobile from ''Series/{{Batman}}'' is seen overhead. Tim Burton would later go on to direct ''Film/{{Batman}}'' and ''Film/BatmanReturns'', as well as co-produce ''Film/BatmanForever''.
* HollywoodHomely: Dottie.
* MemeticMutation: '''[[OhCrap "ANDY!?!"]]'''
* NightmareFuel: Large Marge, as well as Pee-wee's nightmares.
-->"On this very night.. ten years ago.. along this same stretch of road.. in a dense fog just like this.. I saw the worst accident I ever seen. There was this sound.. like a garbage truck.. dropped off the Empire State Building... And when they finally pulled the driver's body.. from the twisted.. burning.. wreck, it looked like.. ''[[http://blogs.sfweekly.com/thesnitch/Large%20Marge.jpg this!]]''"
* SpecialEffectFailure: In the pan-and-scan version of the film, you can clearly see Pee-wee's chain feeding up through the bottom of his bike's saddlebag. This is a common problem with pan-and-scan version of videos, which display more space along the top and bottom of the frame than is visible through a projector.
** The increasingly insane road signs are obviously being rolled past the camera on a track.
** When the convertible is falling, the lack of wind in the closeups makes the fact that Mickey and Pee Wee are sitting in a tilted but stationary car rather obvious.
*** The car might fall under NarmCharm or StylisticSuck since it fits with some of the other, obviously intentional stylistic choices in the movie.
----