!!!All comics:
* AlternativeCharacterInterpretation:
** [[StrawmanHasAPoint With the message Team Plasma spreads in]] VideoGame/PokemonBlackAndWhite, interpretations go from them being a group of {{well intentioned extremist}}s, {{straw hypocrite}}s, {{anti villain}}s, or a mix of the three. How they interpret N is a whole different story.
** Given the large number of variations on the same stories, there's quite a bit of this within the spinoff comics as well. One of the more common interpretations in runs of [[VideoGame/PokemonRedAndBlue the first gen and its remakes]] is the common {{Fanon}} theory of Blue/Gary being Oak's [[TheUnfavorite Unfavorite]] while his grandfather favors the MC appears quite often. Other runs paint him as a flat-out {{Jerkass}}, while others split the difference for a JerkassWoobie.
** Another frequent point of variation: how gym leaders and other trainers respond to death on the battlefield. Any given gym leader could be portrayed as a professional who has accepted death as a natural consequence of battle, a friendly foe who's honestly shocked at seeing their opponent's pokemon die, or an utter {{Jerkass}} who gloats and rubs the loss in their face.
** [[VideoGame/PokemonRedAndBlue Professor Oak, Bill]], and [[VideoGame/PokemonRubyAndSapphire Mr. Briney]] are often portrayed as creepy perverts, as well.
** In ''almost any'' Ruby/Sapphire/Emerald run you read, Norman will be interpreted as a trainer who is so obsessed with Pokemon that he neglects spending time with his family.
** In the same vein, Archie is seldomly portrayed as anything more than [[StupidEvil a moron]]. Maxie has a few moments [[WhatAnIdiot ("How did I not see water is GOOD?!"),]] but he is generally shown as more intelligent and having [[AntiVillain greater thought out motives.]]
** Also in Hoenn runs, Wallace tends to be depicted as CampStraight and in a relationship with Winnona. This is probably influenced by ''Manga/PokemonSpecial''.
* CommonKnowledge: The original challenge did not have "No Legendaries" as a rule (Legendaries weren't caught simply because they weren't the first encounter Nuzlocke ran into in their area), nor was "Blackout/Whiteout = Game Over" part of the original rules. ([[spoiler: Ruby ended the way it did probably because the author felt turning the loss against the Champion into a DownerEnding worked well from a story perspective]]) In fact, "you must nickname all your Pokemon" is generally accepted as part of the core Nuzlocke rules these days, but even ''that'' wasn't the case in the original either. The only two rules the first challenge used were stated in the first panel: "Release a Pokemon if it faints" and "Have to catch the first Pokemon on each route and nothing else".
* ComplacentGamingSyndrome: Plagued with it to a degree. Despite the random nature of the game and how death can result in some odd teams, a ''lot'' of the comic makers tend to make similar choices of starter Pokemon and likely make similar choices on keeping mons if they are able to use them. A Johto locke that picks Chikorita tends up attract intrigue and curiosity since it is a rare occurrence while a Sinnoh locke that picks a Chimchar is regarded as another run-of-the-mill Nuzlocke.
** And most people are almost always going to get, the bug type, the rodent, the bird, and a zubat at some point. Just cause of how common they are.
* CrowningMomentOfAwesome: [[Awesome/NuzlockeComics Here]].
* CrowningMomentOfFunny: [[Funny/NuzlockeComics Here]].
* CrowningMomentOfHeartwarming: And [[Heartwarming/NuzlockeComics here]].
* MemeticMutation: The [[SelfImposedChallenge Nuzlocke Challenge rules]] have since then become ''very'' popular among players.
** "It's all happening for a reason."
* {{Shipping}}: In spinoffs, the MainCharacter with Pokemon Gym Leaders/NPC:
** A LOT of Nuzlockers seem to have waifu-time with their favorite Gym Leaders. Or, more recently, with [[MrFanservice N]]. Here's actually a few Nuzlockers who shipped ''rivals'' with Gym Leaders.
*** Or, if there's more than one rival (like in ''Ruby and Sapphire'', ''Diamond and Pearl'', ''Black and White'', and ''X and Y''), rivals with ''each other''. Kynim's ''Diamond'' Nuzlocke is a noteworthy case of this, as one of the endgame pairings is [[spoiler:the two male rivals]].
* MisaimedFandom: The Nuzlocke challenge itself qualifies--the creator of ''Pokemon'' stated that he wanted the NonLethalKO system in there partly so that the game doesn't become impossible and partly because he didn't want children to grow up associating death with losing a game. Didn't stop this self-imposed challenge from becoming a thing.
* RescuedFromTheScrappyHeap: A pretty normal occurrence in Nuzlocke runs. Due to the rules, players often end up having to use Pokemon they don't normally use, and consequently develop newfound respect and appreciation for them.
* StopHavingFunGuys: It is to be expected, given it is part of the Pokemon fandom, albeit rare. There are some who take the challenge [[SeriousBusiness rather seriously]] and will criticize the teambuilding of other players (if they are sharing the progress and results of their challenge) even though one of the premises of the challenge involves adapting to having no choice on what mons you can use outside of ones given as gifts.
* StrawmanHasAPoint: There's something to be said for N's claims -- that Pokemon battles are cruel, selfish, and only result in Pokemon being hurt -- on a normal playthrough. On a Nuzlocke run, he's pretty much right.
* TearJerker: Whenever a Pokemon dies. [[TearJerker/NuzlockeComics Deserves its own page...]]
* TheWoobie: Chances are you will come across a character who has suffered so many hardships, be it the loss of a friend, a crushing defeat, or even personal matters, that you can't help but want to hold them and comfort them.
* TheyWastedAPerfectlyGoodCharacter: Very often a new team member will be given an interesting and unique personality... only to be either permaboxed or quickly die to a random critical hit. Of course, this is sort of forced by AnyoneCanDie combined with RealLifeWritesThePlot.
* SlidingScaleofSillinessVersusSeriousness: The comics tend to vary from both extremes of this.
* {{Wangst}}: Once you apply FridgeLogic to the Nuzlocke comics.

!!!Original comic:
* AlternateCharacterInterpretation:
** Several of the gym leaders, but Erika as a pot head takes the cake.
** N in White: Hard Mode is [[AdaptationalVillainy much worse]] than {{Canon}}; he's willing to ''kill'' Pokemon just to lecture their owners about how "this wouldn't have happened if you hadn't caught them!"
** Also in White: Hard Mode, Cheren and Bianca being creepy {{psychopathic manchild}}ren who stalked and hounded Hilbert so badly that he ''[[DrivenToSuicide hung himself]]'' just to get away from them--and their reaction to seeing this is simply mild disappointment (with the mildly unsettling implication that this has happened before).
** [[UltimateLifeForm Mewtwo]] goes the exact [[AdaptationalVillainy opposite direction]] of how most fanworks, Nuzlocke or otherwise, portray him. In the games, Mewtwo had very little personality, but the 'dex and most hints from its backstory portray it as an easily angered BloodKnight that takes no greater joy in anything but fighting. The anime popularized a more tragic portrayal of the character, a broken creature that sees itself as an abomination and bitterly hates humanity for making it that way, whilst simultaneously having a cold calculating mind. "Fire Red: Hard Mode" portrays a horrifying merger of the two characterizations, with a highly intelligent and manipulative Mewtwo who not only bitterly hates humanity, but also wants to ''destroy'' them, and displays a sadistic love of killing both humans and Pokemon alike.
* BrokenBase: Episode 8 of White: Hard Mode has been getting this for [[spoiler: having Ruby catch Victini, showing the "No Legendaries" rule is not in effect]], resulting in either people okay with it (mostly due to the mon's personality) or crying "ruined".
** Although to be fair, [[spoiler: the original comic never has never had the "No Legendaries" rule. The reason he didn't catch all the legendaries previously is because they weren't the first Pokemon he encountered in an area. [[LoopholeAbuse Victini was]].]]
* CompleteMonster: Mewtwo is the BigBad of the [=FireRed=] arc. A clone of the legendary Pokémon Mew, Mewtwo [[BeingTorturedMakesYouEvil grew a hatred for humanity due to being tortured]] by one of the scientists who created him. After killing a scientist who took no part in his torture in a rage and destroying the laboratory he was created in, Mewtwo came across Mew, who trapped the former on a deserted island in hopes that it would give Mewtwo time for his anger to subside. Despite calming down and regaining sanity, Mewtwo [[MisanthropeSupreme still held his hatred of humanity]] despite knowing not all of them are cruel, attempting to slowly choke a human who accidentally washed on the island shore to death, [[{{Sadist}} simply because he believes it's been too long since he's killed somebody]]. Looking for a way to break the seal that keeps him trapped on the island, Mewtwo deceives [[TheHero Ruby]] into killing the three legendary birds, who kept Mewtwo's power restrained, by pretending to be the ghost of Ruby's deceased Nuzleaf. After Ruby defeats Gary and is about to be crowned Elite Four Champion, Mewtwo [[MindRape breaks the minds]] of everybody in the stadium except for Ruby, and [[FrameUp frames Mew]] for it, intending for Ruby to kill Mew and completely break the seal. Mewtwo mocks Ruby for being tricked by him and proclaims that he looks forward to enjoying his newfound freedom, [[ForTheEvulz and might as well]] KillAllHumans while he's at it, [[UngratefulBastard promising to kill Ruby last as his way of "thanks"]].
* GrowingTheBeard: The Ruby run was good, but rough around the edges. The Fire Red run saw a general improvement in the art, dialogue, characterization and plot, and was an even better series.
** And it seems like the White arc is starting to grow it even further, by having it in full color, the art being a lot more clean, and not overusing the ''Series/{{Lost}}'' references.
* HarsherInHindsight: Meta-example. On late Feb 2017, the author would had his car [[https://twitter.com/Nuzlocke/status/833569811619684352 broken into]] and have his 3DS and ''all'' his Pokémon games stolen. This meant that that he lost all of his friends in real life whether they lived through the story or not, meaning that in a way, they died for real. The only legacy left of them would be the webcomic itself where he [[https://twitter.com/Nuzlocke/status/833722225106694144 already written down]] the notes for White.
* HilariousInHindsight: In the Fire Red comic, Marowak's ghost is "defeated" in essentially the same way it is in ''Anime/PokemonOrigins''.
* HoYay: Bill and the guy with glasses.
* JerkassWoobie: Ruby is by no means a total jerk, and he warms up to any Pokemon he initially hates, but he's often a bit cynical in some chapters. You'd be too if your own father favored a Slakoth over you, you watched several Pokemon die, [[spoiler:lost all your remaining teammates against the champion, almost lost everyone against another, were used by an all-powerful legendary who proceeded to kill ''another'' party member, and [[FishOutOfTemporalWater flung three years into the future in the aftermath]] without the remaining one of your Pokemon, where the cycle starts again via a certain green-haired Pokemon rights activist.]] Guy's got it rough...
** Blastoise also counts to an extent after all the crap he took from Gary despite being a repeated HeroKiller for [[spoiler:Meowth]] and [[spoiler:Blamo]]. As such, his TheDogBitesBack moment feels all the more cathartic.
** Surprisingly enough, [[spoiler:Ghetsis is this as of episode 11]].
* {{Squick}}/{{Fanservice}}: [[http://www.nuzlocke.com/wp-content/uploads/2010/03/ruby51.jpg Torkoal used Attract!]]
* NightmareFuel: [[http://www.nuzlocke.com/pokemonhardmode.php?p=27 Marowak's Ghost in this comic]], [[spoiler: Mewtwo's wall of Nuzlockers in the Fire Red run]], and as of White, N's [[DissonantSerenity Pidove.]] [[VerbalTic Coo.]]

!!!Hale's Emerald/Platinum Hard Mode:
* AlternateCharacterInterpretation: May goes from a FlatCharacter who didn't get as much characterization as Rival Brandon to a StalkerWithACrush[=/=]{{Yandere}} whose expressions evoke ''VisualNovel/HigurashiWhenTheyCry''.
** Also in Emerald, Archie is a hippie, and Maxie is highly competent - he just wants to give land-dwellers a place to stay whenever Archie manages to flood the world.
** And then there's [[CreepyTwins Tate]] and [[{{Twincest}} Liza]]... even their Pokemon were freaked out.
** In Platinum, Stanley is an abusive boyfriend who tries to keep ShrinkingViolet Bern cowed.
%%* BaseBreaker: May
* EnsembleDarkhorse: His depiction of May earned her an instant fanbase and DracoInLeatherPants status.
** The run itself gained a lot of attention from the site as a whole, to the level that they filtered Nuzlocke into ''Hale''locke on the day of the Emerald Run's last update.
* FunnyAneurysmMoment: May was treated as an IneffectualSympatheticVillain and her threats of murder aren't taken very seriously, even when she kills grandma Winstrait. [[spoiler: But then she finally manages to kill Django...]]
* HarsherInHindsight: In Hale's comic, Hale tells May that she should kill herself. [[spoiler: Guess what she tries to do later on.]]
* HilariousInHindsight: [[http://i1.wp.com/hardycases.com/wp-content/uploads/2013/02/page12.jpg This]] strip has Hale hitting his Slugma and burning his hand as a throwaway gag. Fast-forward to VideoGame/PokemonXandY, and playing with Slugma in Pokemon Amie results in the same thing.
* ShipToShipCombat: Of a sort; the fight was between Hale/May and Hale/''nobody''.

!!! Apocalypse Johto:
* FridgeBrilliance: Giovanni is startled when [[spoiler: Candace disguised as a Rocket]] greets him with "Hail Giovanni!", because [[spoiler: he's not the leader of Team Rocket any more. She blew her cover.]]
* MoralEventHorizon: Candace breaking and entering into the Olivine Town lighthouse, [[spoiler:now a shrine to honor Jasmine who sacrificed herself to allow the rest of the town to survive]], by way of Forretress' Mirror Shot to get the badge, [[spoiler:considered a sacred object by the survivors]], was viewed as this by some people.

!!!Nyachan's Nuzlocke Challenges:
* ShipToShipCombat: So much in her ''Pearl'' run, between Lily/Gary and Lily/Kess, that she had to threaten to holler at the mods in the opening post of her ''Sapphire'' run. [[spoiler:Gary won]], if you're interested, but WordOfGod says the author originally intended [[spoiler: Lily to end up with Kess]] before doing a total 180, which was probably the cause of this.

!!!Lyrax's Gold Run:
* MoralEventHorizon: Lyrax's Gold run immediately establishes the rival as irredeemable when he [[spoiler: steals ''both'' of the leftover starters, apparently just so he could tell Lyrax "Don't follow me or I'll ''kill'' one of them." He does]].

!!!In Azza's Run:
* MoralEventHorizon: Charmeleon (and his trainer as well) make it clear they're well over the line when Raticate argues that the murder of innocent Pokemon was completely unnecessary, and demands to be released from the team. They do so, but then Charmeleon claims that since Raticate is now a wild Pokemon, he can do whatever he wants, and very brutally murders Raticate as well.

!!!ShiningAce's Nuzlocke:
* NightmareFuel: This comic's version of Giovanni is basically a mass-murderer who's ''proud of it''. He gets several terrifying NightmareFace shots too.
* {{Shipping}}: Notable as [[http://s7.zetaboards.com/Nuzlocke_Forum/topic/8415965/1/ ShiningAce's Nuzlocke]] he is [[spoiler:in an arranged marriage with Erika]]
** [[spoiler:And then he met Sabrina...]]
** Lampshaded in [[http://img5.imageshack.us/img5/3182/curseofextra8.png this]] extra comic.

!!!Manic's Screenshot Runs:
%%* BaseBreaker: Anemoi from "Spirit Electrum" quickly established himself to be this.
* NauseaFuel: [[spoiler: The ways Stan is described as having killed the gym leaders in "Super Stan's Super Awesome Adventure!"]]
* TheScrappy:
** Beat from "Der Gnizalb" quickly established herself as this due to being a bigger Jerkass than Anemoi and for telling [[TheHeart Bump]] to kill himself.
** From "Dark Stars," Sugar the Smoochum acquired this status due to her obnoxious behavior and turning Sophie against Manic.

!!!Kit's Nuzlocke Adventure:
* AlternateCharacterInterpretation: Cynthia as a raging AlphaBitch who mocks Kit whenever she can. [[spoiler: To the point where secretly a grown up Angelica from Rugrats!]]
** Silver as just a nice guy everyone hates for no reason. He noticed Elm drop a starter's Pokeball and tried to give it back, Elm accused him of stealing, and he fled.
%%* EnsembleDarkhorse:
%%** Ashes, lampshaded in every later run.
%%** Felony the Liepard.
* MoralEventHorizon: Kit considers Kyogre killing his Grumpig to be this, even nicknaming the legendary "Murderer"
** Baskerville manipulating Fortune into hugging her in frount of Lyra, after finding out Lyra had a crush on him, was probably the point she earned her Scrappy status below. [[spoiler: She's killed off pretty soon after, only expressing remorse for doing this when she's about to die.]]
* NightmareFuel: The Black run, due to it being the interpretation of the glitchiness that occurred when the author used cheats to sequence break in the game.
* TheScrappy: Baskerville. Initially she seemed like a well-meaning jerk, but later she was proven to be pure jerk. [[spoiler: She did get a bit of an AlasPoorScrappy moment when she died though.]]
%%* TheWoobie: Silver

!!!Jet's Black Nuzlocke
* AlternateCharacterInterpretation: While most people like to portray N as a PsychopathicManchild and play up his innocence, creepiness or [[PureIsNotGood creepy innocence]], this one portrays him as extremely serious and rational, evidently playing up how seriously he takes his status as Team Plasma's leader. He retains his social awkwardness, though.
* NightmareFuel:
** [[spoiler:Broccoli and Bottles' deaths were as horrifying as they were heartbreaking.]]
** The [[http://jetsblacknuzlocke.com/comic/episodes/ep019.html nightmare]] Jet has just before her battle with [[ThatOneBoss Lenora]]--if the savage looking [[YouDirtyRat Watchog]] and [[HellHound Herdiers]] ''eating'' Firedog, Lilly and Leer don't scare you, than the downright ''demonic'' looking version of Lenora looming over them and laughing maniacally will. And then [[spoiler:Broccoli]] and [[spoiler:Bottle]]'s ghosts show up...

!!!Other comics:
* NightmareFuel The Unnamed Protagonist from [[http://s7.zetaboards.com/Nuzlocke_Forum/topic/8579823/1/ Pitch Black]] is a [[NightmareFuel terrifying]], evil, sadistic protagonist. From the beginning it's established that he's NOT a good character, (Considering we see his first activities IMPALING POKEMON ON HIS PICKET FENCE). From then on, any Pokemon he catches he forces to fight and brutally murder his friends and family and sees them nothing more as tools, even going as far as to [[spoiler: KILL his own Pokemon]]. Basically, he's an even worse version of Paul. His Oshawott, Icarus, MAY be just as bad.