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* AccidentalInnuendo / GettingCrapPastTheRadar: Cherry Jubilee is styled like a "madam" of the Old West, and being "boss of Cherryhill Ranch" may give a few different visions of [[TheOldestProfession what actually goes down there]]. Not to mention she can always use "[[AccidentalInnuendo a pony with quick hooves and a strong back]]".
* BestKnownForTheFanservice: Derpy getting her first prominent role and having dialogue far overshadows anything that happened in the rest of the episode.
* BrokenBase: This episode has joined the likes of "[[Recap/MyLittlePonyFriendshipIsMagicS2E8TheMysteriousMareDoWell The Mysterious Mare Do Well]]" as one of the series' most divisive episodes. Strangely, it wasn't due to the events of the episode itself, but Derpy's cameo, both before and ''after'' the infamous edits made to her appearance. Was it a nice tribute to the fans of the show, obnoxious pandering to those fans, possibly offensive to disabled people, or all of the above? Things got at least 10 times worse after the edits[[note]]Which edited out Derpy's name, changed her voice, and switched her crossed-eyes to correctly aligned eyes.[[/note]] that were made. [[RuleOfCautiousEditingJudgement AND THAT IS ALL THAT WILL BE SAID ON THE MATTER.]]
** There are some who consider the episode to be very tedious due to it's predictability as well as the contradiction of Applejack being the Element of Honesty. (and to a lesser extent the contradiction of Rainbow Dash being the Element of Loyalty due to leaving Pinkie Pie and Rarity behind)
*** While there are some fans who want both the defenders and the detractors of Rainbow Dash and Applejack's actions to ''get over it,'' stop demonizing Dash, and move on.
* EpilepticTrees: A lot of people began making theories that TonightSomeoneDies or gets PutOnABus with this episode, presumably because the word "last" is used as part of the episode title. "The Last Roundup" is a term best associated with death. [[note]]"Last roundup" tends to be used for when a cowboy is soon to die.[[/note]]
** That said, the [[Recap/MyLittlePonyFriendshipIsMagicS2E15TheSuperSpeedyCiderSqueezy6000 very next episode]] was already confirmed to be another Applejack based one, so it was easy to disprove any such theories, though considering all info given about that episode before this episode mention the Apple Family, but not Applejack herself.
** The fans, after hearing Rarity's vow of vengeance, quickly assumed that she was the reason why Rainbow Dash was in the hospital in "Read it and Weep". When the episode premiered, it turns out to be her crashing.
* FanficFuel:
** The method of the revenge Rarity promises against Rainbow Dash is a popular fanart topic.
** Pinkie and Rarity's trip back on the handcar. The two characters are rarely paired up with each other and the stories usually play out like the WesternAnimation/FamilyGuy episode ''Road to Rhode Island''.
** Pinkie's reaction to a "Pinkie Promise" being broken...
* HarsherInHindsight: Compare this episode where Granny Smith says "Our little bushel just lost one apple" (in reference to Applejack's desertion) while Big Macintosh and Apple Bloom sob in the background. Mildly sad at the time, but absolutely heartbreaking after "Apple Family Reunion", [[spoiler: where it's revealed, albeit implicitly, that Applejack's parents are dead.]]
* SugarWiki/HeartwarmingMoments: Fluttershy and Twilight Sparkle: "We can always find a way to fix the hole in the roof, but if you don't come back who's going to fix the hole in our hearts?" Even the tomboyish Rainbow Dash shed a tear at that line.
* HilariousInHindsight: Back in "[[Recap/MyLittlePonyFriendshipIsMagicS2E5SisterhoovesSocial Sisterhooves Social]]", we had Rarity calling out Sweetie Belle for causing damage, and this time it's Rainbow Dash scolding Derpy. Extra points for a variant of "don't do anything at all" being said each time. ''[[Recap/MyLittlePonyFriendshipIsMagicS2E3LessonZero Extra]]'' extra points for Rarity and Derpy sharing a voice actor.
* StopHelpingMe: Derpy's efforts in setting up Applejack's sendoff party end up doing more damage than anything else, and are what ultimately set off the events of the episode.
* SuspiciouslySimilarSong: The music behind AJ's practice run in the ColdOpen is the ''{{Series/Bonanza}}'' remix first heard in [[Recap/MyLittlePonyFriendshipIsMagicS1E10SwarmOfTheCentury "Swarm of the Century"]].
** Also, when the other Mane 6 ponies are searching for Applejack, the background music sounds very similar to the Hammer Bros. theme from VideoGame/SuperMarioBros3.
** For the backing music of the cherry conveyor belt scene, there's a play on the well-known "WesternAnimation/LooneyTunes machine music" (more specifically, the second movement of [[StandardSnippet Raymond Scott's "Powerhouse"]]).
* TearJerker: The Apple family's reaction to Applejack not coming back is pretty heartmelting (particularly Apple Bloom), as is Applejack admitting in defeat her reason for avoiding her friends, she didn't come first in ''any'' event, and considers herself a failure because of it.
** Becomes even ''more'' of a TearJerker, as well as Harsher in Hindsight, when watching the episode after seeing "Apple Family Reunion". In that, everypony in the Apple Family makes an appearance... except [[spoiler: Applejack's parents, who [[StarsAreSouls confirmed to be dead]]]]. Now watch that aforementioned scene again ad realize how heartbroken the Apples must have been at the thought of [[spoiler: losing ''another'' family member]]...
* {{Wangst}}: It can be a little hard to feel pity for Applejack being rude to her friends, breaking a Pinkie Promise, and leading them on a dangerous desert chase, just because she [[spoiler: didn't win first place.]]
** JerkassWoobie: Both the above and the below tropes paint Applejack in a flawed, but sympathetic light. Her actions are all in good intentions but she has a prideful and misguided direction to it.
* TheWoobie: [[spoiler:Applejack. Just listen to how downright ''heartbroken'' she sounds revealing how she failed! She feels like she's a complete and utter failure for not getting the awards the town expected her to.]]
** The poor telegram pony; it was his birthday! Fortunately, Pinkie eases his disappointment.