* SugarWiki/AwesomeMusic: The theme used for the gurney ride in ''The Mad Doctor'' (reused as the final boss theme).
* BreatherLevel: ''Moose Hunters'' is significantly easier than ''the Mad Doctor'' or ''Lonesome Ghosts''.
* DemonicSpiders: The Weasels in ''the Prince and the Pauper'', most definitely. They take several hits, are difficult to beat if you run out of marbles, and can hit you from far away. [[Film/WhoFramedRogerRabbit Too bad you can't just make them laugh themselves to death.]]
* EnsembleDarkHorse: Just like his original short, the Mad Doctor (or rather, [[IAmNotShazam Dr. XXX]] in the original short) is remembered quite fondly in particular. Helped out by the fact that the cartoon and character is by far and large the most obscure cartoon in the game.
* GeniusProgramming: The Sega Genesis version of the game features Mode 7-style perspective effects in a number of the levels... produced entirely in software, as unlike the SNES, that system has no hardware support for such effects. In fact, the SNES version even ''lacks'' one of these levels.
* HilariousInHindsight: A video game featuring a FunnyAnimal star of a LongRunner franchise going through places he's been to before and allying with his past self or selves. [[VideoGame/SonicGenerations Remind you of anything?]]
* MostAnnoyingSound: When Mickey is hit, he will say either "Ow!" or "Uh-oh!". Considering how [[NintendoHard difficult]] the game is, these phrases quickly become ''very'' annoying, especially the latter.
* {{Narm}}: In the Playstation version of the game, Mickey will for some reason ''always'' enter an animation that has him checking his watch when he finds Pluto at the end of ''the Mad Doctor'' stage, making him seem completely uninterested in having found his pet.
* TheyWastedAPerfectlyGoodPlot: A minor example. It's a great game and a good 65th anniversary tribute to Mickey, but one wonders why there was no level devoted to the "Sorcerer's Apprentice" from Disney/{{Fantasia}}. That's one of Mickey's most iconic moments.
** Perhaps because that was a segment from a [[DisneyAnimatedCanon Disney Animated Canon]] compilation film and not a proper standalone Mickey Mouse short.
*** That and there was already a game featuring the Sorcerer's Apprentice aka the Fantasia Genesis game.