* AlternativeCharacterInterpretation: [[spoiler: The final scene in the episode "Show and Tell" has Big Jet apologise to the Little Einsteins for nicking their belongings, and then express sadness over not having anything to present for show and tell, only to be cheered up when Rocket gives him a sombrero to fool around in. While it can be assumed that the episode was trying to go for a DefeatMeansFriendship moment and imply that Big Jet has [[HeelFaceTurn made the decision to reform after acknowledging his actions]], it's still somewhat open-ended, as the episode was his last appearance before the series ended, and at no point in any of his other appearances did he ever apologise or show remorse for his wrongdoings, so it's hard to not rule out the possibility that he could have been emotionally manipulating the team just to get them on his side.]]
* FridgeLogic: An episode that features a "DontWakeTheSleeper" scenario requires the viewer to clap the beat of the music with the kids; wouldn't clapping technically wake the creature up?
* GatewaySeries: For many 2000s kids, ''Little Einsteins'' was the catalyst that got them interested in ClassicalMusic. A lot of people who watched ''Little Einsteins'' when they were young still think ClassicalMusicIsCool, and some even went on to study music or become professional musicians.
* HilariousInHindsight: "Pluto, it's time for you to go back where you belong!" In an episode that aired 10 days shy of Pluto's reclassification...
* MemeticMutation: When the show's theme was reworked into a hip hop beat, it gained loads of popularity on places like Vine, likely as a result of the PeripheryDemographic.
* PeripheryDemographic:
** There are thriving communities of ''Little Einsteins'' fanfiction on the Internet.
** Since this is aimed at young kids, you'll likely have a lot of parents or babysitters watching the show with them, causing many of them to become fans of the show.
* RetroactiveRecognition: In [[SameLanguageDub the UK version]], Leo was voiced during the first season by Piers Stubbs, who a decade later would voice [[CreepyChild Eli]] in ''VideoGame/MetalGearSolidVThePhantomPain''.
* RoboShip: June/Rocket, especially in the episode "The Glass Slipper Ball", thanks to June dancing with Rocket, ''a sentient rocketship''. She even gives him a kiss on the nose in the end. It was not long before some fans took it to new levels and paired them together...
* SignatureSong: Good luck finding any person who grew up in the 2000s who doesn’t know the theme by heart.
* SongAssociation: While many of the classical pieces used in ''Little Einsteins'' are well-known tunes [[StandardSnippet that people are sure to hear often in their everyday life]] ("StandardSnippet/OdeToJoy", "[[Music/WolfgangAmadeusMozart Eine kleine Nachtmusik]]", "[[Theatre/PeerGynt In the Hall of the Mountain King]]", "[[Music/LudwigVanBeethoven Für Elise]]", "The Blue Danube", "[[LohengrinAndMendelssohn Wedding March]]", etc.), there are also more obscure pieces that many kids who grew up with ''Little Einsteins'' are only familiar with because of the show, such as Schubert's "Trout Quintet", Smetana's "The Moldau", Dvořák's "Humoresque No. 7", and, for a generation unfamiliar with ''Series/AlfredHitchcockPresents'', Gounoud's "Funeral March of a Marionette".
* ToyShip: Leo/June is the most popular ship among fans, mainly due to them being close in age and the tender hug they shared in "Ring Around the Planet". Quincy/Annie is also commonly shipped, [[ShipMates especially by Leo/June fans]], due to the longing looks she often gives him in episodes like "Whale Tale".
* UnintentionallyUnsympathetic: [[spoiler: Big Jet apologising to the main characters for stealing their things at the end of "Show and Tell" is supposed to be seen as sweet and commendable, as well as a clear indicator that he's moving past his previous wrongdoings, but there are a few things about him doing that which could rub some people the wrong way, particularly regarding the fact that the episode is the last time we see him in the show, and we aren't even given anything to so much as indicate that he was undergoing a change of heart beforehand. Up until this point, all of his appearances revolved around him [[TheBully bullying the Little Einsteins]], [[DickDastardlyStopsToCheat sabotaging their chances of winning things]], resorting to whatever measures he can to try and get to something before they do, and generally [[{{Jerkass}} making their lives more difficult than they should be]] (not to mention the fact that "Show and Tell" isn't even the first time he's stolen something from them), and he's done it entirely out of spite as a means of trying to get back at them for narrowly beating him in "Hungarian Hiccups". And in addition to that, we never see him apologise or show any remorse for any of the things he did previously (a few of which were debatably ''worse'' than what he did in this episode, like smashing Rocket's flight button just to ensure he doesn't beat him in "The Great Sky Race Rematch" or tearing up Annie's song in "Annie, Get Your Microphone" for the exact same reason), resulting in his apology here feeling out-of-nowhere and possibly even insincere. It doesn't help that the team, in spite of all he's done to them, [[EasilyForgiven let him off the hook just because he said sorry for this one thing]], which is then followed by Rocket giving him a sombrero to use for show and tell. It all has the potential to send a bad message to the show's target audience like "It doesn't matter if you repeatedly bully someone, because all will be forgiven if you apologise to them", which in real life is ''very'' untrue.]]