* BestKnownForTheFanservice
* BigLippedAlligatorMoment: The "PANCAKES!" scene and the rabbit surgeon. Big surprise the first one was added in at the last minute when Eli Roth saw the little kid practising tae kwon do.
** Some people also considder the [[CoitusEnsues impromtu sex]] between Paul and Marcy one of these, as it seems so unlikely that people in that situation would actually start screwing. However, it is probably more blatant [[FanService Fanservice]] then Big Lipped Alligator. Besides, it also has reprecussions in the plot [[spoiler: mainly, Paul contracted the disease from mixing bodily fluids with Marcy, who was infected.]] Also a deleted scene foreshadowed it a little where Jeff was sleeping alone while Marcy slept next to Paul.
* EnsembleDarkhorse: Bert tends to be the most popular of the college kids amongst fans
** Deputy Winston, the party cop, counts as well
* InferredHolocaust: [[spoiler: The last shot is of the first shown victim lying in the river and bleeding into the water. It pans over to a truck of bottled water, presumably filled with CoolClearWater (see above) from the river.]]
** The ending animation shows a trucker drinking Down Home Spring Water. He pees in the bottle and throws it at some dumb kids in a convertible. [[spoiler:The kids are heading to New Orleans for Mardi Gras.]]
* MemeticMutation: PANCAKES!
** "Ooooh! Faced!"
* NauseaFuel: A lot. Why do you ask?
* NightmareRetardant: Don't sit next to Dennis.
** All he wanted was some PANCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAKES.
* SignatureScene: Dennis's scene.
* {{Sequelitis}}: So bad that the director refuses to be associated with it.
* {{Squick}}: A lot. Why do you ask?
* ViewerGenderConfusion: The "Pancakes" kid is a boy, named Dennis. However he has very long hair and is apparently prepubescent, so many people mistook him for a girl.
* WhatAnIdiot: Winston. Aside from being creepy as fuck, he does not seem to think clearly either. A sick and disoriented [[spoiler:Paul]] encountered Winston partying in the forest, and knocked him out when he heard that all infected persons are to be shot on sight. He then stumbled onto the highway, and a truck-driver took him to that local doctor's office, where the police located him. As he was too sick to talk, he could not communicate to the officers that one of the infected bodies had fallen into the local water reservoir, and hence infected it. To find out what he tries to say, the doctors advise that the police brings him to the county hospital, who actually has the capabilities to treat the flesh-eating bacteria. Deputy Winston is chosen to transport him there. Yet, he is vengeful for being hit over the head by him earlier.
** '''You'd expect:''' That he will complete his task without hesitating. [[spoiler:Paul]] is quite possibly the key for scientists to analyse this virus further so they are prepared for future infections. Apart from that, [[spoiler:Paul]] still has something '''very''' important to say.
** '''Instead:''' He gives a shit. He is only out for revenge, and dumps [[spoiler:Paul]] [[DownerEnding in the closest river]]. [[AndThatsTerrible And that was bloody stupid]]. He pretty much is solely owe for everything that happened in the sequel and [[InferredHolocaust after that]]. He actually dumped a guy with a highly aggressive and quickly spreading bacterial infection in a river, which co-incidentally leads back to that town and the CoolClearWater bottling factory. He should really have known that. Apart from that, there wasn't much of a reason in the first place. Oh, [[KarmaHoudini and he got away with it]].