* BigLippedAlligatorMoment: The scene where Dr. Pretorius shows Dr. Frankenstein a bunch of small humans he's created and placed in jars which has no analogue in the original novel.
* CrazyAwesome: Dr. Pretorius (Ernest Thessiger) is a CampGay MadScientist who grows [[PeopleJars tiny people in jars]], does not so much tinker in God's domain as wage open war against him, dreams of creating a race of monsters and interrupts his grave robbing to hold cozy picnics with Frankenstein's monster in the crypt. To put it another way, this is a MadScientist who scares ''Dr. Frankenstein''.
* EnsembleDarkhorse: Despite only having a short appearance at the end of the movie, the Bride has become one of the most famous Universal monsters.
* EvenBetterSequel: While Universal's first ''Film/{{Frankenstein|1931}}'' is well-regarded, this sequel is considered even better by many.
* HoYay: The blind man and the Monster have their moments.
* IAmNotShazam: Lord Byron himself actually calls the monster Frankenstein in the prologue, and Pretorius is quick to dub the new female monster "[[TitleDrop the Bride of Frankenstein]]". Although perhaps he was using the word "of" in the same manner as in [[Creator/MarvelComics "Monster of Frankenstein"]] (This may also be an artifact left over from the original plan to have Elizabeth's heart implanted into the Bride, which would have made the name literal.)
* MoralEventHorizon:
** The Monster reaches this early in the film when he first strangles an elderly couple in their home for [[ForTheEvulz absolutely no reason]] and then brutally murders a young girl. [[WhatMeasureIsAMook We don't actually get to KNOW these people, so we can forgive the Monster for these fiendish acts]].
*** In the original script, Creator/DwightFrye's character Karl is the one who kills the old couple (who turn out your be his uncle and aunt) and then lets the Monster take the blame.
** As for Dr. Pretorius, he crosses it when he [[ManipulativeBastard manipulates the monster]] into his services and has Elizabeth held for ransom in exchange for Dr. Frankenstein's assistance in his experiment.
* OneSceneWonder: The eponymous Bride appears onscreen for all of about three minutes, but definitely makes an impression.
* TheScrappy: Minnie, the annoying, loud-mouthed housekeeper.
* SignatureScene: The Bride getting created.
* UncannyValley: The Bride. She looks normal, except for her hair and a few scars, but the way Elsa Lanchester portrays her the wide-eyed yet expressionless face, the stiff arms, the sharp, jerky motions of her head, the ''hiss'' she actually makes the Bride seem less human than Karloff's Monster.
* ValuesDissonance: The hermit teaches the Monster to smoke, saying it is "good". This film was made before the harmful effects of smoking were known.
* VisualEffectsOfAwesome: The sequence with the homonculi holds up well almost 80 years later.
* TheWoobie:
** The poor old hermit, who is so happy to finally have a friend, only to be separated from him almost immediately and having his home burned down shortly after.
** The elderly couple at the beginning, who previously lost their daughter to the Monster and end up losing their lives here because of the man's thirst for closure. Although he ends up [[NoOneCouldSurviveThat being proven right]] when he tries to make sure that the Monster is really dead, he ends up going by himself and puts himself in a precarious position for the Monster to drown him like he did with his daughter. And then his wife ends up helping the Monster get out of the sewer (thinking it's her husband), only to be violently flung down when she recognizes him and screams.