* HarsherInHindsight: The whole film is about Barbara's life being in danger. Her voice actress Mary Kay Bergman committed suicide a few years after making the film.
* HoYay: A huge YMMV regarding this one, but some of Freeze and Dr. Belson's interactions.
* HilariousInHindsight: A pop remix of the standard "Am I Blue" plays on the radio in Barbara Gordon's room. In the ''JusticeLeagueUnlimited'' episode "This Little Piggy", Batman goes onstage in a nightclub and sings - you guessed it - "Am I Blue".
* JerkassWoobie:
-->'''Barbara:''' How can you treat people this way?\\
'''Freeze:''' It's the way they've always treated me. Nora was the only good thing in my life... and they took her from me. I don't care what I have to do to get her back.
* NightmareFuel: Mr. Freeze's revenge on the submarine crew. Imagine returning to the cave where your team's sub is parked after a scientific expedition...only to discover the frozen corpses of your comrades.
** Being kidnapped for an involuntary organ transplant.
*** Adds a fine layer of FridgeHorror when you realize that there were seventeen other people on the list that were compatible with Nora's blood type. Seventeen ordinary people who aren't superheroes, who don't spend their time with the world's greatest detective, and wouldn't have the police commissioner sending all available forces to look for them. If Mr. Freeze had chosen anyone else on that list, they would have been brutally killed.