* BigLippedAlligatorMoment: A few of Stan Lee's cameos, such in ''Star Trek'' where he's [[spoiler:teleported into the transporter room, dressed in a tuxedo and singing "Hello, My Baby!"]], or in the second ''Iron Man'' episode, where he's [[spoiler:sitting on a tiny planet in outer space]].
** Captain America somehow pulls out and blows a conch shell when Bruce Banner [[HulkingOut Hulks out]] in the bowling alley.
* EarWorm: The music that plays during the opening credits will become infectious the more frequently you watch, especially since cartoons from season two onward play it at both the beginning and the end.
* HilariousInHindsight: After the release of ''Film/IronMan3'', it seems ironic that Iron Man's second episode [[spoiler:makes the Mandarin resemble Creator/BenKingsley.]]
* NauseaFuel: The {{Vomit Indiscretion Shot}}s.
* {{Squick}}:
** Wolverine's attempt to eat sushi off of his blades causes him to slice his face open. [[GoodThingYouCanHeal Good thing he can heal]].
** In ''Ant-Man'', [[spoiler:TheReveal that the female ant had sex with an unconscious Ant-Man.]]
* TheWoobie: Most of the superheroes, but Bruce Banner especially. His episode might as well be titled ''Bad Life''.