* ''{{Franchise/Metroid}}'': Samus Aran is a girl but wears a full armor suit. There's an old troll question that goes like this: "Is Metroid's gun attached to his arm or his suit?"
* Slippy Toad in ''VideoGame/StarFox64''. The fact he was voiced by a high-pitched female did not help matters at all.
** Parodied in one ''VGCats'' [[http://www.vgcats.com/comics/?strip_id=103 comic]], where Leo and Aeris try to see which bathroom Slippy uses. Doesn't work. "It's peeing in the fucking break room!"
** However, in ''VideoGame/StarFoxAssault'', Slippy has a male voice. It's only the N64 title and arguably ''Adventures'' where the confusion happens.
* This has happened to Tails from ''Franchise/SonicTheHedgehog'' a few times, even though in the first games in which he speaks, he was actually voiced by a boy.
** It's AllThereInTheManual, of course, since his given name is the unambiguously male [[PunnyName Miles Prower]].
** Doesn't help any that in the Spanish word for "tail" is feminine. And this hit the dub like a bolide hit Earth 65 million years ago.
** A large portion of this is due to the {{urban legend|s}} that Tails was originally designed as a girl but was changed at the last minute. Said legend is prevalent both in Japan ''and'' the US, leading to double the confusion. Whether or not it's true has yet to be proven, but most informed fans believe it quite false.
*** If you research his design process, you find that the two-tailed fox design was always male, and that Sonic was always intended to have a sidekick the same gender as him.
** It REALLY doesn't help that Tails' looks on the title card of ''VideoGame/SonicTheHedgehog2'' looks really, really feminine.
** Nor does it help that Tails is currently voiced by a girl.
** Due to his dreadlocks, Knuckles is often mistaken for a girl.
* Due to no voices, prepubescence, hair clips, and small graphics, some viewers originally thought Pat from ''VideoGame/MegaManStarForce'' was a girl. The Japanese names for the character, Tsukasa and Hikaru, are both [[GenderBlenderName androgynous names at best]]. In addition, while "Tsukasa" is usually written in kanji for boys and hiragana for girls, Tsukasa Futaba TookAThirdOption and writes his name in katakana. English names "Pat" and "Rey" attempt to keep this impression (or lack of it). You'd almost think it was deliberate...
** Not to mention that the first time fans got a look at him was during the opening of the anime, where his hair was incorrectly colored blond. Needless to say, it didn't help.
** Zero in ''VideoGame/MegaManX'' comes equipped with long hair and, well, there's no other way to say it -- booblights, causing some confusion until ''Mega Man X4'' added his ridiculously manly voice, and ironically enough X's ridiculously girly voice.
*** Zero was always intended to be male. That bit of female!Zero art drawn as a joke just as R20 was about to be released in Japan didn't help matters.
** Even worse was Harpuia in the ''VideoGame/MegaManZero'' series: a bishonen named after a always-female monster with a soprano voice in the first game. Later games pitched it down an octave or two.
** And then there's [[http://images4.wikia.nocookie.net/megaman/images/8/8d/CubitFoxstar.jpg Cubit Foxtar]], which the [[AllThereInTheManual Complete Works]] states as ''male'' (an oblique reference to ''kitsune'', well, pretending to be women), and [[http://images2.wikia.nocookie.net/megaman/images/0/01/PolerKamrous.jpg Polar Kamrous]], who has a gruff voice to match '''her''' (this time the ''story'' stated it explicitly) build, from ''Zero 3'' and ''Zero 2'', respectively.
** The first images of the "enemy Rockmen" in ''[[VideoGame/MegaManZX ZX Advent]]'' showed them in armored form, leading many to believe that Atlas (Model F) was a man and Tethys (Model L) was a girl; the names didn't help (Atlas was a Titan, Tethys a Titaness in mythology), and neither did the fact that they were boss-{{exp|y}}ies of Fefnir and Leviathan, also a man and woman, respectively. When the unarmored pictures and gameplay videos came out, Atlas had a visible bust and Tethys sounded like a boy, albeit young. They rapidly became known as "Trap" and "Reverse Trap" in the fanbase. The dub makes Tethys sound older and more boy-like, at least.
*** In the original ''ZX'', if you never hear Hurricaunce's voice, you could think she's a guy.
** Serenade from the ''VideoGame/MegaManBattleNetwork'' series, one of the strongest Navis in the world. [[ShesAManInJapan His gender was changed for the US version]] and kept vague for several years in Japan, leading to a lot of heated debate. [[AllThereInTheManual Finally a profile book was released where his profile stated he was "a male Net Navi with the appearance of a young girl"]].
** But the worst is probably Lumine from ''Mega Man X8''. Very female voice, long light-purple hair, thin feminine body, yet other characters always refer to him as a "he".
*** Similarly for Optic Sunflower, he has a very feminine voice, but is referred to as male in-game.
* Eric Lecarde in the Japanese and European releases of ''VideoGame/CastlevaniaBloodlines'' is a bishonen-style pretty boy with a decidedly feminine outfit (a very short red tunic and white leather boots, and unlike the Belmonts, there's no armor or animal hides to toughen it up). The only tipoff you get is the conspicuous lack of breasts. The US version gives him a more masculine face to make it clearer that no, he's not supposed to be a chick.
* Yoshi in ''Franchise/SuperMarioBros'' -- "she" ''laid eggs'' for crying out loud. It ''really'' doesn't help that "Yoshi" is used to refer to either one individual or his entire species. Birdo is even worse though - a male crossdresser who spits eggs out of its... nose? Though given that the CREATORS have flipped on what gender the latter is...
** FanWank often involves thinking of the "eggs" as more like poop, given the method of their production. Apparently Yoshis learned their combat techniques from monkeys.
*** WordOfGod says male Yoshis lay exploding eggs used for combat, females lay actual eggs, and the Yoshi usually seen with Mario and friends is in fact male.
*** Conflicting evidence comes from Japanese text from a ''VideoGame/SuperSmashBros''' trophy, claiming that Yoshis all have no gender.
* Watt, the living spark in ''VideoGame/PaperMario'', has no obvious gender identifiers. The text usually uses feminine pronouns for Watt, but switches to male in at least one place.
* ''Franchise/TheLegendOfZelda's'' Link is not a stranger to this trope, thanks to being the main character in a game [[ProtagonistTitleFallacy called Zelda]] and people occasionally mistaking his tunic for a skirt. It doesn't help that he tends to be [[{{Bishonen}} really pretty...]]
** Sheik is definitely a contender for King/Queen of this trope. WordOfGod has it s/he's female, but would you know that on your first playthrough of ''[[VideoGame/TheLegendOfZeldaOcarinaOfTime Ocarina of Time]]'', what with the Nintendo 64's limited graphical horsepower? [[BrokenBase S/he single-handedly divided the entire Zelda fanbase on the issue of their gender, and continues to do so today]]. Of course, this one is interesting because Sheik is actually the (quite obviously female) Zelda- the real issue is "is she disguised as a man, or does she transform into a man?" The manga said she transformed (more like brainwashed, if you pay attention), while ''Super Smash Bros.'' manual said it was just a disguise. What version counts as WordOfGod depends on who you ask.
*** It ''really'' does not help that Ruto actually calls Sheik a man at one point, or that Sheik's concept art looks pretty masculine...
** Due to the artstyle making no body-built differences between male and female characters, quite a few players also thought Tetra, (who's basically Sheik's ''[[VideoGame/TheLegendOfZeldaTheWindWaker The Wind Waker]]'' counterpart, minus the mysteriousness) was a boy, right until Link's little sister Aryll exclaimed "How terrible! The ''girl'' fell into the forest!!". Tetra is the only one in the game who suffers from this, since she's the only female character who is wearing pants.
** The guards found in the N64 Zelda games are a strange case: the [[http://www.zeldawiki.org/images/4/48/Hyliansoldieroot.png official art]] makes it easy to tell, but the actual in game model, due to graphical limitations, has a slender build with what appears to be a pronounced bustline, lipstick, and tight short pants.
** There's a double example in ''[[VideoGame/TheLegendOfZeldaTwilightPrincess Twilight Princess]]'' with the shaman [[http://www.zeldawiki.org/images/2/25/Renado.png Renado]] and his daughter [[http://www.zeldawiki.org/images/c/cb/Luda.png Luda]]. Because of his robe and her cowboy-like outfit, they could be easily seen as mother and son.
** One Game Informer preview of ''[[VideoGame/TheLegendOfZeldaSkywardSword Skyward Sword]]'' described Demon Lord Ghirahim as "a skinny, ghostly ''female''".
*** Then again, Ghirahim doesn't have a gender.
** Yuga in ''VideoGame/TheLegendOfZeldaALinkBetweenWorlds'' looks a lot like a DistaffCounterpart of Ganondorf (and is often confused as a female 'witch' like characters as a result) but is actually male.
** Within hours of the reveal of the currently-untitled ''Zelda'' game for WiiU, there were a number of arguments as to whether or not Link is female in it. His {{Bishonen}} attributes seemed to have been [[UpToEleven made even more prominent]] in this installment, with rather distinct eyelashes and a ponytail.
* The mandatory ''Franchise/FinalFantasy'' examples
** Most protagonists all the way back to Cecil from the fourth game, who, upon armor removal, had ''long flowing hair and a tiara''. Furthermore, he's got black lipstick in his concept art. Not to mention a fair few of the antagonists, until the tentacles and spikes and roots come out.
*** Speaking of Cecil, in Nintendo DS remake of his ''VideoGame/FinalFantasyIV'' game, he is given light blue armor and a blue hairband. He's still got the lipstick in the CGI, though. The voiceacting kinda helps, as well.
*** It's actually Lampshaded in Troia, when a drunken pub-goer mistakes him for a hostess.
** Minwu from ''VideoGame/FinalFantasyII'' is frequently thought to be female, despite being called a man when he is introduced, the robes, veil and [[WhiteMage powerful healing abilities]] are major reasons why.
** Kuja from ''VideoGame/FinalFantasyIX'', anyone? He's the ''dude'' back on the main page. He even has feminine hips and waist, which arguably falls into "cheating." The only thing keeping him from being a woman, visually, is that we can't see the boobs or junk.
*** This is lampshaded when [[spoiler:he confronts Queen Brahne after she (expectedly) turns on him, and she says, "It's about time you showed your girly face here."]]
*** And lampshaded again in ''VideoGame/DissidiaFinalFantasy'' where Penelo gives her descriptions of all the characters and claims that she dislikes Kuja since no boy should look prettier than she does.
*** The fact that his art by Yoshitaka Amano makes him look like he's wearing black lipstick and mascara (but then again, that's just part of his art style) doesn't help either. Surprisingly enough; Nomura actually made him look MORE masculine for ''Dissidia''.
** For that matter, at least one ''FFIX'' player mistook Zidane for a girl. But considering that [[spoiler:they're brothers, maybe androgyny runs in the family.]]
*** [[spoiler:All the Terran clones look identical to Zidane, even the girls, so no surprise there.]]
*** [[spoiler:When Zidane specifically asked another of his kind whether they had males or females, they responded by saying that yes they do because asexual beings would lack the capacity to adapt to new environments.]]
** Chances are that 75% of the people who have played ''VideoGame/FinalFantasyXII'' have mistaken Larsa for a girl. Alas he is a boy. A very girly boy. With very pretty shoes. A very twelve year-old boy.
*** It doesn't help that you can take him along on the hunt for Orthros, a creature that only appears with an all-female party. (Yes, you could also take the very manly Reddas with you much later, but Larsa's with you at the right level.)
** Sorceress Adel from ''VideoGame/FinalFantasyVIII'', obviously. Despite being a sorceress, the first reaction is generally "That's a man," due to her tattoos and overall masculine appearance. Not helping matters is the fact that [[DistressedDamsel Rinoa]] spends the entire battle attached to her torso, Adel siphoning HP out of her by thrusting her pelvis forward.
** Ramza from ''VideoGame/FinalFantasyTactics''. Interestingly enough, Ramza's technically androgynous when it comes to the game's statistics system: he gets physical growth like a man and magical growth like a woman.
*** Although in the EnhancedRemake, he sounds masculine.
** Many fans of ''VideoGame/FinalFantasyTacticsAdvance'' thought that Marche looks like a female, with his near-feminine hair and shy personality. Luckily, when the bullies teased Marche for being "quiet like a little girl", Ritz's response made the fact that Marche is indeed a boy.
*** It doesn't help that Marche's hair actually looks ''just'' like Ramza's.
** And after so many {{Bishonen}} protagonists throughout the series, there were some that originally thought [[VideoGame/FinalFantasyXIII Lightning]] was yet another girly ''male'' protagonist!
** ''VideoGame/CrisisCore'': [[http://images5.fanpop.com/image/photos/25100000/Genesis-Rhapsodos-genesis-final-fantasy-7-fan-club-25151825-337-272.jpg Genesis]] is a man, however much the picture might fool you.
* In the ''VideoGame/{{SaGa}}'' game ''[[VideoGame/SaGa3 Final Fantasy Legend III]]'', a boss named Matreiya is referred to as "he" but take '''one''' look at the sprite and you'd bet that "he" looks like a "she"! S/he could '''easily''' pass for [[AntonyAndCleopatra Cleopatra]].
** Partially justified in that some depictions of the Buddhist [[http://en.wikipedia.org/wiki/Maitreya Maitreya]] could pass for female by Western standards.
* Zelos in ''VideoGame/TalesOfSymphonia'' upon first glance when you see him on the cover of the game... and sometimes second and third glances too. Once you meet him in the story and figure out he's a male-voiced HandsomeLech, you might be in shock for three more hours of gameplay. It probably also doesn't help that Zelos wears pink, and in the west, that's considered a feminine colour. (The RapunzelHair, too.)
** Don't forget Corrine. Girly voice and name, cute and cuddly ball of fluff, multi-colored, Corrine's gotta be a girl, right? ''Nope''. The little fox is a male. However, Corrine can also go into [[ViewerGenderConfusion an even bigger gender debate]] when you take in two factors, the manga [[spoiler:and the fact he becomes Verius. Verius is obviously male and outright proven so, especially when playing the sequel, and this is yet another factor that can confirm Corrine is male because he ''is'' Verius. However, the manga depicts Corrine differently where Verius is an entirely new entity sharing Corrine's likeness only in their life force and appearance. They also confirm ''Corrine'' is female and ''Verius'' is male. Oi.]]
*** Granted, a skit in the game states that Summon Spirits actually ''have'' no gender and only appear in either a male or female form for sake of convenience.
* Ion from ''VideoGame/TalesOfTheAbyss''. Not only is he incredibly androgynous and has a very female-like voice, other people refuse to use pronouns to refer to him and instead say 'Ion'.
** The Japanese version is ''slightly'' more difficult to confuse because his voice is more masculine and he uses a masculine pronoun to refer to himself... Mind you, there's a whole fetish around [[{{Bokukko}} girls who do that]], but most of those oddly act ''less'' feminine than Ion.
** Jade gets a bit of this now and then, either from the especially feminine concept art or from the viewer only seeing a skit face, which shows the character from the shoulders up (he's quite pretty). The GenderBlenderName doesn't help matters.
* When gamers first saw the NA cover art of ''VideoGame/TalesOfVesperia'', many assumed that the box was depicting the hero and heroine of the game. Nope. The feminine looking one with the long black silky hair is the ''Hero'' of the game. The Guy with the blond hair is the rival. The actual heroine of the game is on the back of the box.
** This is [[LampshadeHanging lampshaded]] near the begining of the game. If you go into the bar and speak to a certain NPC, he mistakes Yuri for a girl and asks him to join him for some drinks.
* And again, in ''VideoGame/TalesOfGraces''. You could be forgiven for thinking that the tall, beautiful blonde in the opening is a woman, especially since the [[https://www.youtube.com/watch?v=SymLluPY-ig&t=1m31s video and the song]] are cut make it seem like she's [[HoYay Asbel's long-lost love interest or something.]] But no, he's a dude and his name is Richard. [[spoiler: Some people actually interpret him as androgynous anyway, due to the genderless appearance of his Lambda form.]]
* ''VideoGame/FireEmblem'' has had a handful of examples such as Sephiran and Chainey, but the most amazing ur-example is Lucius, with his bright blue eyes, long, flowing blonde hair, and monk robes that made him look like he's wearing a form-fitting dress. Not a single person thought he was a man when they first saw his official art. His gentle, feminine, almost WhiteMagicianGirl personality really didn't help. Nor did his child-bearing hips. Or the fact that his main support conversation (and the only one with a double-ending for Lucius) is with another guy.
** In the case of Lucius, it's even parodied in-game in one of his support conversations, where Serra mistakes him for a girl before he meekly corrects her.
-->'''Serra:''' B-But... to be a monk, don't you have to... be a... well... a guy?\\
'''Lucius:''' Yes. Yes, you do.
** Marth got this by ''Videogame/SuperSmashBros'' fans -- partially due to the unusual first name, which is the male variant of Martha, and partly due to the pretty tiara he wears.
** [[BadassBookworm Soren]] is girly enough to have been repeatedly taken for a {{Tsundere}} SweetPollyOliver. The HoYay with [[TheHero Ike]] doesn't help.
** Rickard from ''Videogame/FireEmblemAkaneia'' is supposed to look like a girl, but in the cases where most female thieves in the series are tomboyish PluckyGirl and Rickard's appearances change [[DependingOnTheArtist depending on the game he's in]], he's easily overlooked compared to the other examples.
* Starlow from ''VideoGame/MarioAndLuigiBowsersInsideStory'' and ''VideoGame/MarioAndLuigiDreamTeam'' has some of this, [[GenderAndJapaneseLanguage at least in the English versions]]. She's just a floaty little ball with feet and a star hovering over her head, and there is only one line referring to herself as female ("NOT how to treat a lady!"). However she has little eyelashes in the official art, but not in-game. ''Dream Team'' has these eyelashes visible during the dream-world sequences, which helps some.
* ''Videogame/KingdomHearts'' always had its share of gender confusion. If you started playing the series with ''Chain of Memories'', it takes quite a while to get everyone's chromosomes right, no thanks to the confusing pixel anatomy and strange, quasi-corset Organization coats making '''EVERYONE''' have an hourglass figure.
** [[http://www.freewebs.com/aqualover/marluxia_2.jpg Marluxia]] was originally intended to be a woman. Due to "her" already slightly masculine appearance and the fact that this trope is par for the course for Square-Enix, they didn't actually have to change anything when they decided to make the switch. Amazingly enough, the Organization features only [[http://www.freewebs.com/aqualover/larxene_2.jpg one lonely female]] (but no shortage of estrogen).
** When the promo images of ''Videogame/KingdomHearts'' were first released, there was a debate on a Disney forum about whether Riku was a boy or girl.
* Rhyme from ''VideoGame/TheWorldEndsWithYou''. Of course, she's a young girl, so unless others are talking about her with pronouns, there's no way to tell. Another example of going by the game cover; it's obvious in-game. Not making it any easier for those playing The Japanese version -- turns out Rhyme is a {{Bokukko}}.
* Kazooie, from ''VIdeoGame/BanjoKazooie'', can be very confusing, given her brash character and utter lack of feminine traits. Rareware apparently got sick of it, though, since TheRevival gave her a few TertiarySexualCharacteristics.
** Many players have mistaken Terry from ''Banjo-Tooie'' as a female, since he gets mad because he thinks you've stolen his eggs, and no gender is immediately given, you'd think that he laid the eggs and is the female. But according to the game's instruction manual, as well as some random dialogue from Zombie Jingaling, Terry's wife has left him, and therefore he is very protective of the eggs.
* Kanan from ''Videogame/SoulNomadAndTheWorldEaters''. In fact, you'll probably need to pick a certain path in the main campaign to find out "she" is really a "he".
* Gunstar Red from ''[[GunstarHeroes Gunstar Super Heroes]]'' is a girl. It's due either by reading the manual or by her gender being expressed ONCE that you would know this.
* Lotte from the obscure SNES game ''VideoGame/ClockTower'' is commonly confused for a guy... Despite having noticeable breasts, [[http://dcj.bluemoonsea.com/articles/lottesgenderexplained.html among other things]].
** This wiki even mentions that "Her name is ''Lotte''." However; chances are most people didn't actually spot that name since the other characters have rather English sounding names (Jennifer, Laura, Bobby, Mary...)
*** ...Char''lotte''?
* ''[[{{Moviebob}} The Game Overthinker]]'' summed Square-Enix's propensity for this rather nicely: "... Oh, wait, I think that's supposed to be a guy... ''Fucking Squaresoft!''"
* Ron Delite from ''Franchise/AceAttorney''. He has all sorts of female characteristics, such as a pointy "egg" head shape, hair like that of [[Franchise/StarWars Princess Leia]] and somewhat of a female face. Doesn't help that he's very kind, gentle and shy, and that his Mask☆De Masque costume is very husky.
* Nick and Deanna from the ''[[Videogame/ShiningForce Shining Force Gaiden]]'' series are some of the worst examples of this, ever. Just look them up, and be confused. It doesn't help that Deanna has a name that's a woman's name ''everywhere but'' in this game.
** Khan from ''VideoGame/ShiningForceIII'' was a very effeminate holy man with a hat that covered everything on his head but his face. Also they only other character that used his weapons and fighting style was a girl.
* Calintz, main character of the ''Videogame/MagnaCarta'' game (at least the one released in the West).
** He's also the main of the original PC game... despite it otherwise having a totally different plot and setting. And he was, by reports, ridiculously feminine there, as well. The artist for the series [[AuthorAppeal apparently likes feminine men and ridiculously busty women]].
* ''Videogame/{{Suikoden}}'':
** Secondary characters can invoke gender confusion in the player. Most notable of the examples is Milich from Suikoden 1 thanks to his extremely extravagant fashion tastes, the flower-themed palace, and noblewoman's laugh. Fortunately there is a way to know for sure if one is really that curious.
** Some people had the same problem with Luc and Sasari in ''Videogame/{{Suikoden}} II'', looking at their portraits. The problem was rectified in the sequel, where both men had obviously male portraits and character models.
** While almost immediately referred to as "Prince", preventing any confusion, the hero of ''Videogame/Suikoden V'' is downright girly. Only when you look at him at a certain angle (And while wearing certain clothes) does he even begin to look male. Most of the time, he looks pretty hot.
** When Subala's character art was revealed, most of the fandom assumed that she was a boy due to her completely flat chest. Much to their surprise, she turned out to be a 16-year-old girl.
* The Night Dancer in ''VideoGame/FinalFantasyTacticsA2'' plays with this. He appears to be the first female bangaa ever in the series, but once you try to uphold the law in any mission he appears it quickly turns out that he is, in fact, a crossdresser (and no, the game doesn't generalize by race when it comes to unique characters, even though the law sounds like it).
** The game also lampsahdes the confusion between two [=NPCs=]; a boy thinks the Night Dancer is an old man while another person mistakes the same character as a female. Even your own party makes a remark on the Night Dancer's gender and choice of clothes/make up, to which said character takes offense and claims he is "beautiful".
* The ''Videogame/HarvestMoon''-iverse has Julius, who's... [[http://www.fogu.com/hm/wii/img/preview/char_julie.png Well, just LOOK at him!]] His favorite items are even things like jewelry and perfume! If it weren't for his name, it'd be next to impossible to tell that he's supposed to be a dude. And yes, you can marry him if you're playing as a girl. Sort of a shame, since there'd certainly be players who would marry him if he were in fact a woman. He doesn't even have the name to help him in Japan, where his name is Juli. The whole joke was that Candace/Kotomi was bullied by Juli as a child, and always thought he was a girl. Then she grows up, meets him again after so many years, and is shocked to discover that the girl who bullied her was a boy the whole time.
* There's also Jamie from ''Videogame/MagicalMelody'', who is always the opposite gender of your character, but they have the same sprite regardless (and hides this fact well by having Jamie wear a poncho the whole game).
* In ''VideoGame/{{Fallout 3}}'', many viewers were led to the impression that Fawkes was female, not because of any physical characteristics, but due to misunderstanding a laboratory log in game. After a [[http://fallout.wikia.com/wiki/Talk:Fawkes/Gender bitter, lingering controversy]], stoked by public comment from the voice actor who played Fawkes, the issue was finally resolved by Word Of God.
** Closer observation of the in-game model of Fawkes would have resolved it even sooner: The tattered remnants of his vault suit has seam patterns consistent with the male version of the suit, not the female.
* Thanks to voice acting being brought into the second game, NiGHTS from [[NightsIntoDreams said titular game]] now speaks with a posh woman's accent, causing many people to think that they're a woman ''despite [[WordofGod it being confirmed]] that Nights has no gender''. NiGHTS' voice can be interpreted either as a woman or a young boy; it sounds equally like both.
* Birdo from ''Videogame/SuperMarioBros 2''. While there is more than one member of the species (much like Yoshi), the main one featured in the games is a male...most of the time. Nintendo has [[FlipFlopOfGod changed Birdo's official gender from male to female to]] [[AmbiguousGender not really saying if it's one or the other.]]
* Emilio Michaelov from ''PsychicForce'', at least in the Japanese version, has a very feminime, timid voice, combined with his wings and even androgynious body shape and hair The US voice acting does remove this doubt.
* ''Videogame/DynastyWarriors'' characters:
** Zhange He -- Long hair, sometimes butterfly wings, pink and purple clothes, flowers EVERYWHERE. The only way you can tell his gender is by his voice.
** Lu Xun -- tiny, pretty, big eyes, slight body, looks like a prepubescent girl.
* ''Videogame/SamuraiWarriors'' has Ranmaru Mori (who's even voiced by a woman) and is actually mistaken for a girl by [[ChivalrousPervert Magoichi]]. The newest game brings forth Hanbei Takenaka, who is older than Ranmaru but still looks very feminine. The fact that he's seen disguised as [[DisguisedInDrag a woman]] in his opening doesn't help either...
* Uesugi Kenshin from ''VideoGame/SengokuBasara'', who is '''extremely''' pretty and graceful, has delicate features and is voiced by Creator/RomiPark. Takenaka Hanbe and Mori Motonari have similar problems, as not only are they pretty but they also have rather curvaceous hips.
* Shinta Kikuchi from ''Videogame/OsuTatakaeOuendan''. [[http://lparchive.org/LetsPlay/Ouendan%202/Images/26-shintaface.png Look at him/her]]. He's pretty much [[Franchise/AceAttorney Ron DeLite]] without the Leia-hair.
* ''VideoGame/ThreeInThree''. You might think a talking number would be [[NoBiologicalSex neuter]], not feminine...
* Would you believe [[http://www.nightofthequinkan.com/EnTY/entymain.php?id=67 THIS]], from ''Videogame/TyTheTasmanianTiger'' had me thinking she was male? In all fairness, the voice acting sounds like a man trying to sound female, and bungling it, and from a decent distance, you can't make out the breasts. Also, Ty backs away in panic.
* Arno from ''[[SummonNight Summon Night 2: Swordcraft Story]]''. Is it a boy, or a girl? Th characters never find out, Arno never goes by any sort of pronoun and him/herself a "child of the wind", and doesn't know their own gender...The only clue we ever get is one of the villains shouting "you neutered freak!" And it's not even a big clue at all. Seriously, [[http://farm3.static.flickr.com/2030/2270354388_04c3ef5ed8.jpg?v=0 you tell me.]]
* Mao in ''ShadowHearts: From The New World''. That's a ''man's'' voice coming out of that cat, and yet it's supposedly a female. Since Mao's a cat without any form of TertiarySexualCharacteristics, the confusion gets racked up. (She only refers to her gender a small handful of times, easily missed.)
* ''Videogame/AnimalCrossing'':
** Meet Bob. Bob is a kitty. Bob is purple. Bob loves to wear pretty dresses. Bob is a dude.
** Likewise with Blanc''a'', Gracie and Saharah are male... in Japan. A few villagers also get this trope.
** You can also invoke the trope yourself by changing your hairstyle to one used by female player characters. Sadly, you can't wear a dress unless your character is female to begin with.
* ''VideoGame/{{Touhou}}'':
** [[http://en.touhouwiki.net/wiki/Wriggle_Nightbug Wriggle Nightbug]] from ''Imperishable Night'' is sometimes mistaken for a boy, due to her being one of the few ''Touhou'' characters to wear pants instead of a dress.
** This is not in effect for Mokou from the same game, despite also wearing something other than a dress-- her name (lit. Scarlet Little Sister) gives it away.
** The confusion strikes again, this time with [[http://en.touhouwiki.net/wiki/Shou_Toramaru Toramaru Shou]] from ''Undefined Fantastic Object'', who ''does'' look overly masculine-- tiger stripes and all.
** And for a trip back in time to PC-98 days, we have the samurai [[{{Bifauxnen}} Meira]], whose battle challenge was mistaken by Reimu for a marriage proposition.
* Teepo of ''Videogame/BreathOfFireIII''. Long purple hair, acts like a tomboy, doesn't get referred to using a pronoun until ''literally'' the end of the game. Yes, he ''is'' supposed to be a boy. "He" also ''sounds'' distinctly female as a child.
* Many people confuse Ammy from ''VideoGame/{{Okami}}'' as a male. Issun constantly referring to her as a [[SheIsTheKing god rather than a goddess]] doesn't help.
** The American localization was actually trying to keep Ammy as androgynous as possible, mostly since the United State's primary religion has God to be definitively male.
* [[http://images3.wikia.nocookie.net/__cb20090610212047/umineko/images/2/29/Kanon.jpg Kanon]] from ''Videogame/UminekoNoNakuKoroNi'' can be easily confused for a woman when you first see him. [[http://images1.wikia.nocookie.net/__cb20101003014206/umineko/images/4/47/Lion.png Lion]] from EP 7 is even worse. But in this case it's intentional, since [[AmbiguousGender nobody knows what Lion's gender is]].
* You can make this happen ''yourself'' with the custom players in ''Videogame/BackyardSports'': you can make a boy wear girly clothes and sound like a girl, or a girl look and sound like a boy.
* ''Videogame/EarthBound'':
** Giygas falls under this. Officially, Giygas is a "he". But, thanks to a random NPC in the game, several members of the fandom have mistaken "him" for a female.
** In Japan, Giygas/Gyiyg/whatever has an ambiguous gender; it's never defined as explicitly male or female, but that's much easier to avoid in Japanese than English.
** Ness falls victim to this for the Videogame/SuperSmashBrothers players who haven't played Videogame/{{Earthbound}} before.
* King croacus from ''VideoGame/SuperPaperMario'' is such, despite his obviously masculine title, he has girly eyelashes, a beauty mark, and big red lips.
* ''VideoGame/{{Grandia}}'': Thanks to the way he's drawn in promotional art (and his appearance in general), you could easily mistake Justin for a girl until you learn his name or hear his voice.
* ''[[Videogame/{{Wartech}} Wartech: Senko no Ronde]]'': Cuilan. [[http://hg101.kontek.net/senkonoronde/Cuilan.jpg Here]] [[http://hg101.kontek.net/senkonoronde/Cuilan-B1.jpg HE]] [[http://hg101.kontek.net/senkonoronde/Cuilan-A3.jpg is]].
* Vivian, from ''VideoGame/PaperMarioTheThousandYearDoor''. In the Japanese release, she's transgender and her sisters treat her poorly because of it (arguably also out of jealously that a male could be so much more effeminate than they are). Like Birdo, this was changed for the US release, making her female from the start.
* ''Franchise/{{Pokemon}}'':
** Bugsy.
*** Somewhat long hair, too young to make out sexual characteristics, unusual name that isn't easily gender identifiable, rather [[WhoWearsShortShorts ambiguous clothes]]. Hey, your guess is as good as mine.
*** Supposedly male...but yeah, the fact that his Pokémon are all female doesn't help (enemy trainers usually have Pokémon that are their own gender.)
** Will of the Elite Four has the same issue as Bugsy--despite being male his entire team is female. Both his in-game sprite and the Sugimori art also give him HartmanHips.
** ''Manga/PokemonSpecial'' actually made fun of this.
** Also, [[PlayboyBunny Lo]][[KillerRabbit punny]]. There is a 50/50 chance that the Pokémon is a male/female, and it doesn't help that (s)he has a [[FetishFuel feminine appearance]]. It helps even less that most people say the name is a caricature of ''{{Playboy Bunn|y}}ies''.
** A better example would be the Ralts line. While Ralts itself looks like a small child with a gender-neutral figure, the evolutions are ballerina based. However their "clothing" is feminine. As in "female tutu" and "dress" feminine. They gained a male counterpart the next gen, however you still have to go through the Kirlia stage and the males evolve into Gallade by a (somewhat rare) evolutionary stone; also, male Gardevoir still exist.
** Even odder, Gardevoir has a very masculine name in Japan. Sirknight, which has both "Sir" and "knight" in it (while knights can be female, we typically think of male ones); though it may also refer to "come, Knight". To make it even worse, [[VideoGame/PokemonXAndY Mega Gardevoir]] appears to be wearing a ball dress.
** And then there's the [[VideoGame/PokemonBlackAndWhite Generation V]] counterparts [[ElegantGothicLolita Gothita, Gothorita and Gothitelle]], which have a 25% chance of being male.
** There's also Hitmonchan, who looks like a female boxer (with breasts and a short skirt) but is actually always male.
** Silver, your Johto rival, invokes this. In the remakes they gave him a more masculine appearance however he still invokes this due to his young age, {{Bishonen}} good looks, and long hair.
** Remember ThatOneBoss duo [[VideoGame/PokemonRubyAndSapphire Liza & Tate]]? They're androgynous-looking twin children with long hair, and have feminine and {{Gender Blender Name}}s respectively. Tate is a boy.
** Wallace. With that pose, cyan styled hair, and fabulous clothes, anyone could mistake him for a girl and never know the truth.
** [[http://bmgf.bulbagarden.net/showthread.php?t=69617 Cheren]] from VideoGame/PokemonBlackAndWhite. His Sugimori art makes him less ambiguous.
*** N appears to be an ordinary {{Bishounen}}, but his character art uses Whitney's girly skipping pose, which has given some fans mixed signals.
** This also could have occurred to the translation team for ''[[VideoGame/PokemonRubyAndSapphire Ruby and Sapphire]]'', who named a Team Magma admin with a slightly ambiguous-looking sprite the very feminine "Tabitha." Unfortunately, [[GenderBlenderName Tabitha is a man]].
** [[VideoGame/PokemonDiamondAndPearl Team Galactic Commander Saturn]] is male. However, his tunic is sufficiently ambiguous that the writers of ''PokemonDiamondAndPearlAdventure'' portrayed him as an attractive girl with a visible bust for a couple of chapters before suddenly realizing their mistake and subsequently pretending it never happened.
*** Additionally, Saturn uses a female Toxicroak as his main Pokemon (as noted, [=NPCs=] usually use Pokemon of their own gender); however, this is read more as a ShipTease with (female) co-commander Mars, as [[UncattyResemblance their signature Pokemon resemble each other's hairstyle]].
** Braixen and Delphox are based on witches--Braixen, in particular, on the CuteWitch--but, like all other starters, has a 7-to-1 ratio of males to females.
* US players of the original ''PhantasyStar'' had a couple of examples. First there's the {{Bishounen}} Noah who is referred to by both gender pronouns thanks to the BlindIdiotTranslation. Thankfully the re-release on the Gameboy Advance fixed it so he's always referred to as a male. Then there's the cat-like [[PunnyName Myau]], whose gender is never revealed in the Japanese version nor have I ever heard an WordOfGod on the topic.
* Flea from ''VideoGame/ChronoTrigger''. [[DudeLooksLikeALady He's so girly]] that the entire party (in particular Marle) is shocked to learn he's a guy. Even his official artwork shows him as buxom. Of course, Flea's a shapeshifter who believes that "power is beauty", so he could be a man who looks like a woman, or a man who turned into a woman, or who the hell knows what.
* In ''VideoGame/ChronoCross'', Flea is wearing a pink-and-white [[{{Joshikousei}} schoolgirl uniform]] with a cute pink hairstyle.
* Invoked in ''VideoGame/BaldursGate''. An early sidequest has you hunting down a [[AxCrazy deranged]] necromancer serial killer, and when you track him down, he confuses you for his dead parent...''of the opposite gender than you''.
* BioWare tends to avert this by giving the female characters either scanty clothing or large breasts. The only exceptions are Jack, who they make a point of not using gender-specific pronouns for her until you see her rising out of cryo, and Shale, who is a golem and doesn't give you any physiological clues as to her gender (in fact, even ''she'' is surprised by the GenderReveal).
* Brenda from ''VideoGame/MuscleMarch'' is officially female, but given that she's as ridiculously muscular as the other characters (with the exception of Rossi the polar bear), and the MachoCamp overtones of the rest of the game, it's easy to be confused.
* Ranmaru from ''Sakura Taisen V''. Always referred to with male pronouns... but only referred to with any pronouns starting in Chapter 6, when he's been appearing all game. With a high-pitched voice and very effeminate mannerisms. Given that he's most likely the same Ranmaru mentioned under the Art section, it's not too surprising.
* Prince Pixel of ''VideoGame/GraffitiKingdom''. Granted, it's because he's a little boy and he's almost immediately called "Prince", but the fact that he has those huge eyelashes coupled with his female sidekick having a ''deeper voice than him'' makes it hard to believe that you're playing as a prince.
* {{Kirby}} might very well be this, given that even the Japanese don't seem to know the character's gender. Most derivative translations default it as a dude, though.
** Coo the Owl looks very feminine, but is actually a male...[[ShesAManInJapan at least outside of Japan, anyway]].
* [[http://i73.photobucket.com/albums/i212/Kerrah_photos/Ehl.jpg Ehl]] from ''{{Solatorobo}}'' is a boy, to the surprise of many a gamer. The pink-ish fur and stockings don't exactly help with the issue. [[spoiler:Subverted, Elh is actually a girl who acts like a boy, they find out her true gender thanks to a ShowerScene in Chapter 4.]]
** Then there's [[CampGay Alman]], who looks kinda female, uses somewhat affectionate/cutesey terms when talking, but is refered to using male pronouns.
* In the first ''VideoGame/{{Tekken}}'', Kunimitsu was an AmbiguousGender PaletteSwap of Yoshimitsu with the same voice, but was made distinctly female in the second game.
* ''GoldenSunDarkDawn''
** When the first playable Water Adept was revealed, fans were confused by "Crown", who wears an absurdly short tunic and leggings similar to Karis's, has [[YouGottaHaveBlueHair blue hair]] in a bowl cut, fights similarly to Mia, and has [[VoiceGrunting high-pitched squeaks for a voice]]. "Crown" was [[DubNameChange renamed Rief]] for the dub, which makes his gender a little more clear: the [[DudeLooksLikeALady dude looks like a little girl]].
** Then the second playable Water Adept was revealed. A slender, {{Moe}} [[TheIngenue Inegenue]] whose name in Japanese is phonetically identical to "[[SpellMyNameWithAnS Harmony]]". And an introductory storyline including an extended ShirtlessScene. The European official site cited the [[DubNameChange redubbed]] Amiti as a girl for a while, and we can't really blame them for being confused. [[spoiler: It might run in the family; Amiti's biological father is heavily implied to be Alex, making Amiti and Rief second-cousins. Once you're looking for it, they even resemble each other.]]
* ''DarkSouls'': It's easy to mistake the androgynous Gwyndolin for a woman. It's how he was raised.
* In universe example with Minogame from ''VideoGame/{{Hellsinker}}'' whose gender is described as impossible to tell from appearence. [[spoiler: He[=/=]She is a {{Hermaphrodite}}]].
* In the original ''VideoGame/RainbowSix'' trilogy, male and female operatives look identical in-game.
* Dorrie from ''VideoGame/SuperMario64'' falls under this. Cutesy-looking plesiosaur with a feminine sounding name? Sounds like a girl, right? Nope, Dorrie's a boy. All official descriptions of the character refer Dorrie as a "he".
* In the ''VideoGame/EtrianOdyssey'' series, you always have two male and two female character designs for each class. In several cases, at least ''one'' of the male designs could easily pass for a girl, while there are a few potential {{Bifauxnen}}. The Survivalists and Troubadours from ''I''[=/=]''II'' are prime examples, are are the Hoplite of ''III'' and the Swordsmen of ''IV''. It's extremely likely this is far more ''deliberate'' than many other examples here, as well, since the player is encouraged to make up their own story in their head about their team in order to immerse themselves more fully in the game, so some portraits can be whatever the player ''wants'' them to be.
* In ''VideoGame/{{Startopia}}'', the Dahanese Sirens are the only race with [[HumanAliens human-like features]], except for their dragonfly-like wings. They are also the only race to have visible sexual dimorphism. You have the larger broad-shouldered and bare-chested ones as well as the daintier swimsuit-wearing ones. Oh, and by the way, the first ones happen to be the females of the species, while the latter are the males. Of course, you won't know that without [[AllInTheManual reading the manual]].
* Kamek in ''VideoGame/YoshisIsland'' and ''Franchise/SuperMarioBros''. I mean, magic using character who rides on a broomstick? Well, a whole bunch of fans seem to think he's female, either because he looks like a WickedWitch more than a wizard or is easy to confuse with either Kammy Koopa from ''VideoGame/PaperMario'' or Kamella from ''VideoGame/SuperMarioGalaxy''.
** Antasma from ''VideoGame/MarioAndLuigiDreamTeam'' comes under this. Probably because he looks a lot like the (female) Cackletta from ''VideoGame/MarioAndLuigiSuperstarSaga'' and has a name which could easily fit a female villain. And hey, he's only the second male antagonist in a series which before now has mostly had all female ones.
* ''VideoGame/FreddiFish'' was mistaken as being a male by several younger ones, mainly because she has a name more commonly heard in boys. She has a very feminine voice though, especially in relation to her much younger friend Luther. She's also identified as a female multiple times, including in the very opening scene of the fourth game. Some never caught on though.
* ''PuyoPuyo'' character Oshare Bones is sometimes mistaken as a female, especially by Japanese fans as he uses feminine speech and pronouns in said language. Not only that, but he's also very CampGay, and seeing as he's a skeleton character with highly stylized anatomy, there really aren't any physical cues either. His English dubbed voice is easier to guess, however, since it sounds like a stereotypical gay male.

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