[[quoteright:350:https://static.tvtropes.org/pmwiki/pub/images/twilight_refrain.png]]
''[=TWilight refrAIN=]'' is a vertically-scrolling doujin shooter by AQ Loop released at Comiket 74 for Windows [[UsefulNotes/IBMPersonalComputer PCs]] in 2008.

Players take the role of one of three pairs of characters, one character being present during a given time but they can swap places with each other, and weave through a series [[BulletHell danmaku]] across multiple stages. Each duo has a "Balanced", "Attack", and "Defense" styles that affect their attack power and their ability to use special attacks. The game also features branching paths at certain points of the game that can affect the outcome of the story.

A fan-made English patch by Tenka Seiha can be downloaded [[http://games.seiha.org/viewtopic.php?f=13&t=489 here]] (requires v2.00).

See also ''VideoGame/TwilightInsanity'', this game's predecessor, and ''VideoGame/AlternativeSphere'', a prequel to the series.

!![=TWilight refrAIN=] features examples of:
* AlwaysIdenticalTwins: Ririka (older sister) and Rurika Mikahashi (younger sister).
* DudeLooksLikeALady: Narumi Kirishima, due to his [[ViewerGenderConfusion effeminate]] appearance.
* HalfIdenticalTwins: Narumi (older brother) and Mizuki (younger sister) Kirishima are fraternal twins.
%%* MoreDakka
%%* MultipleEndings
* OlderThanTheyLook: Despite Narumi's looks, he is older than Mizuki.
%%* ShootEmUp
----