* ''WesternAnimation/TheSimpsons'':
** Lenford "Lenny" Leonard and Carlton "Carl" Carlson. When the show spoofed ''Theatre/{{Hamlet}}'', these two were even cast as Rosencarl and Guildenlenny. And then [[Theatre/RosencrantzAndGuildensternAreDead they die]] five seconds after being named.
** Sam and Larry, the ''other'' two patrons at Moe's. You know, the trucker with glasses and the bald guy in the orange jacket who (a) isn't Homer, and (b) looks either really depressed (like "about-to-cry-over-his-miserable-life" depressed) or really drunk. [[ChuckCunninghamSyndrome They [Larry and Sam] were around in the early episodes, but were quickly replaced by Lenny and Carl]], so don't feel bad if you've been introduced to ''The Simpsons'' via the newer episodes and have never heard of either Larry or Sam.
** Patty and Selma may come off as Those Two Twin Sisters. Especially in the early seasons they were hardly ever separated and sport similar hairstyles and wear similar clothes.
** Eugene and Rusty, Springfield's "town perverts" from "Bart After Dark".
** The cops Eddie (the cop who looks like an OffModel Mr. Burns) and Lou (the black cop with the Popeye arms).
** Bart's school friends from the first two seasons, Richard and Lewis.
** Rod and Todd Flanders, the two sons of Ned Flanders. Hard to distinguish them from each other.
* Officers Smitty and URL in ''WesternAnimation/{{Futurama}}''.
* ''WesternAnimation/TheFairlyOddParents'':
** Timmy's best friends Chester and A.J. who, true to form, get their own ''Theatre/RosencrantzAndGuildensternAreDead''-type episode.
** Timmy's "back-up" friends, Sanjay and Elmer.
** The rich jerks, Tad and Chad.
* Pete White and Master Billy Quizboy from ''WesternAnimation/TheVentureBrothers'', who are also business partners and roommates in addition to being super-scientists. In fact, most of the series' characters are foils for one another; Doctor Venture and Brock, The Monarch and Dr. Girlfriend, Hank and Dean, Watch and Ward, Monarch Henchmen 21 and 24, etc. In DVD commentaries, Doc Hammer and Jackson Publick point this trope out and confess that this is because whenever they're stuck or bored while writing they just make up two characters who are basically each other and goof around doing voices as them.
* ''WesternAnimation/CodeLyoko'':
** The two {{School Newspaper Newshound}}s Milly and Tamiya.
** Sissi's two cronies, Hervé and Nicolas.
** Starting Season 3, Hiroki and Johnny also fit the mold.
* Flem and Earl from ''WesternAnimation/CowAndChicken''.
* Several such pairs exist in ''WesternAnimation/TransformersAnimated'': Blitzwing and Lugnut, Bumblebee and Bulkhead, and Mixmaster and Scrapper, and Snarl and Swoop. In the ''Transformers'' comics, Rack'n'Ruin of the Wreckers, who were bonded together in a desperate attempt to save their lives. The fact that they basically have one name (and it's never specified which is which), ''and'' that they receive basically zero characterization even as a pair makes them a perfect example.
* Sheen and Carl from ''WesternAnimation/JimmyNeutronBoyGenius'' and ''WesternAnimation/TheAdventuresOfJimmyNeutronBoyGenius''.
* Mr. Small and Mr. Nosy from ''WesternAnimation/TheMrMenShow''.
* ''WesternAnimation/FamilyGuy'':
** Brian and Stewie have played this role in episodes where they are the focus.
** Cleveland and Quagmire fit Those Two Guys until Quagmire started getting his own episodes and Cleveland [[WesternAnimation/TheClevelandShow got his own show]].
* ''WesternAnimation/PhineasAndFerb'':
** Baljeet and Buford have become this quite quickly, with an interesting FriendlyEnemy dynamic as well.
** Major Monogram and Carl.
** Sometimes, in episodes that focus on Candace, Perry or Doofenshmirtz, Phineas & Ferb themselves get demoted to this.
** Two guys that remain minor from beginning to end on the show are the nameless husband and wife. They're in several episodes for the sole purpose of the same RunningGag. The wife will say, "I can't believe you bought a [fill-in-the-blank product] [fill-in-the-blank product rental/selling place] that didn't have any [fill-in-the-blank products]. What, did you think [fill-in-the-blank products] would just fall from the sky?" Then, the [fill-in-the-blank product] will indeed fall from the sky, usually landing on top of the wife.
* Joey, Jeffy, and Jamie from ''WesternAnimation/{{Daria}}''. They pretty much exist just to remind us that Quinn is attractive when she doesn't have a [[GirlOfTheWeek Boy Of The Week]] around to do it instead. In fact, they're SO Those Two Guys, Jamie is [[RunningGag usually called something else]], only to fruitlessly repeat that his name is Jamie.
* The Badly Drawn Brothers from ''WesternAnimation/AvengerPenguins''.
* Svatislav and Dimitri from ''WesternAnimation/CountDuckula''.
* ''WesternAnimation/RockyAndBullwinkle'' have Edgar and Chauncey, two guys who occasionally comment on the action.
-->'''Edgar:''' Now there's something you don't see every day, Chauncey.\\
'''Chauncey:''' What's that, Edgar?\\
'''Edgar:''' A [unusual sight].\\
'''Chauncey:''' I don't know, Edgar. [punchline]
* ''WesternAnimation/BeavisAndButthead'' is an entire show dedicated to this trope.
* Jody, Ophelia, and Roger are often demoted to Those Three Guys in ''WesternAnimation/TheLifeAndTimesOfJuniperLee''.
* Stan and Koji from ''WesternAnimation/ObanStarRacers''. They're mechanics, wisecrackers... and [[HeterosexualLifePartners almost nothing else.]]
* ''WesternAnimation/ReBoot'':
** The [[FanNickname British Bi-Nomes]].
** One episode has Data Nully and Fax Modem.
** Hack and Slash are this, particularly in the first two seasons.
* Occasionally, [[CaptainObvious Dooley]] and [[{{Jerkass}} Clark]] in ''WesternAnimation/KingOfTheHill''.
* Yuzu and Nonki of ''WesternAnimation/MaryokuYummy'', so much so that the official site has one profile for the both of them.
* Al and Moo Sleech from ''WesternAnimation/{{Doug}}''.
* ''WesternAnimation/MyLittlePonyFriendshipIsMagic'':
** It introduces Snips and Snails, a pair of excitable boy unicorns, in the episode "Boast Busters".
** [[FanNickname Lyra and Bon Bon]] were put side-by-side in the background often since their color-schemes complimented each other. The [[PeripheryDemographic fans]] noticed and they became this thanks to a combination of AscendedFanon and HoYay.
** Twilight Sparkle and Spike have this chemistry at times, usually in episodes where they are supporting characters.
** The show also occasionally likes to pair certain ponies together for particular episodes, even if these examples aren't nearly as consistent due to spotlight rotation. Rainbow Dash seems to form a good few of them (usually with Applejack or Fluttershy).
* ''WesternAnimation/SouthPark'':
** Craig and Clyde.
** Token as he's oftentimes seen with Clyde and Craig. Plus the things that make him stand out (being rich and being black) are purely superficial, and he is otherwise the most normal kid.
* ''WesternAnimation/TimonAndPumbaa''.
* Jean and Paul, the two gay inmates from ''WesternAnimation/{{Superjail}}''.
* ''WesternAnimation/VeggieTales'':
** Jimmy and Jerry Gourd.
** {{Funny Foreigner}}s Philippe and Jean-Claude, the French Peas.
* ''WesternAnimation/KaBlam'':
** Henry and June.
** Sniz and Fondue.
* ''WesternAnimation/{{Recess}}'':
** The Diggers (Sam and Dave).
** In the main six, T.J. and Vince are considered Those Two Guys.
* Mervis and Dunglap from ''WesternAnimation/CatDog''. They also count as [[OnlySaneMan the two only sane guys]].
* Whizzer and Dipstick in ''[[Disney/OneHundredAndOneDalmatians 101 Dalmatians: The Series]]''
* Larry and Steve from the first season of ''WesternAnimation/{{Rugrats}}''.
* Reggie and Bruno Bullnerd from ''WesternAnimation/ChalkZone''.
* Lucius and Samy from ''WesternAnimation/JimmyTwoShoes''.
* Howie and Octo from ''WesternAnimation/AlmostNakedAnimals''.
* Slip and Slide from ''WesternAnimation/CoconutFredsFruitSaladIsland''.
* Chip and Skip from ''WesternAnimation/CampLazlo''.
* Rex and Starla from ''WesternAnimation/NapoleonDynamite''.
* Sancho and Pedro from ''Anime/TheMysteriousCitiesOfGold''.
* Frizz and Nug from ''WesternAnimation/TheDreamstone''. Rufus and Amberley occasionally act as this as well, depending on which side is getting the most SympatheticPOV.
* Katie and Sadie from ''WesternAnimation/TotalDramaIsland''.
* Jang Keng and Tekirai from ''WesternAnimation/HiHiPuffyAmiYumi''.
* Meeker and Snerd on ''WesternAnimation/BobbysWorld''
* In the ''WesternAnimation/TeenTitans'' episode "Things Change", the girl who may or may not be Terra is flanked by two unnamed schoolgirls who seem to be her friends.
* In ''WesternAnimation/{{Asterix}} and the Vikings'', Asterix and Obelix take this role, with Justforkix as the main character. This is particularly silly, since Asterix's name is in the title of the film, and Justforkix was only a (to be fair, strong and memorable) side character in ''Asterix and the Normans'', the comic the movie is adapted from. One reviewer on Website/ThatGuyWithTheGlasses complained it was extremely strange since Asterix and Obelix are better-written, better-animated and designed, and easily the most interesting and likeable characters in the film, yet are [[SpotlightStealingSquad relegated to backup for a whiny brat who gets no comeuppance]].
* Youngmmee, Sue, and Jasper in ''WesternAnimation/LittlestPetShop2012''. Of these three, Youngmee gets preferential treatment by the writers by virtue of being neighbors with Blythe, the protagonist.
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