* Manny from ''WesternAnimation/IceAge'' fits this description, especially in the Original movie, when he is morose and grieving over the loss of his family.
* {{Droopy}} always seemed to be in a really sad and gloomy state. When he was happy, [[IsThisWhatAngerFeelsLike he would just say "I'm so happy..."]] in his usual morose tone. When sufficiently provoked, he would just say, [[PreAsskickingOneLiner "You know what? That makes me mad,"]] [[OhCrap and]] [[LetsGetDangerous then]] [[BewareTheNiceOnes beat the other guy to a pulp.]] Sometimes throwing him miles and miles away.
** Subverted when he won a large sum of money. He smiled from ear to ear, and leaped across the screen. This actually was how he ended several cartoons, bouncing and cheering madly, then returning to his sad state, looking at the camera and saying, "You know what? I'm happy."
*** This itself was subverted in the ''WesternAnimation/HarveyBirdmanAttorneyAtLaw'' episode "Droopy Botox." Droopy sues his plastic surgeon for malpractice after his face freezes into a perpetual grin, and when he loses the case as a result of Harvey exhibiting Droopy's signed indemnity waiver in court, his weeping grin haunts Harvey throughout the episode.
* Toot from ''WesternAnimation/DrawnTogether'' is an example, though she is so bitter and repulsive that she really garners no sympathy whatsoever.
* Twinkle the Marvel Horse in ''WesternAnimation/DaveTheBarbarian''.
* Happy Time Harry in the ''WesternAnimation/AquaTeenHungerForce'' episode "Dumber Dolls" takes this to comically dark extremes. He's a children's action figure who is depressed, addicted to pain pills, has a pile of unpaid "Action Bills" as his main accessory, occasionally practices self-harm with a knife that's ''permanently'' attached to one hand and routinely threatens to commit assault or suicide. In later appearances, he mentions he's on probation too (and missing a meeting with his P.O. by participating in the villain's ZanyScheme). Somewhat of a deconstruction. He shows you what kinds of things need to happen to a guy for him to have such a negative outlook on life. And by "things" I mean "Wife kicked him out, numerous health problems, overdue child support, overdue bills... this can go on. It certainly doesn't help that he took to the bottle to alleviate the pain he faces.
* Stork in ''WesternAnimation/StormHawks''.
** Mentioned or at least implied to be a cultural trait common to ''all'' Merbians. Terra Merb is so riddled with dangerous creatures, dangerous terrain, dangerous weather, and dangerous combinations of all three that Merbians have simply come to expect the worst out of everything, since it usually happens to them. It's so bad that when something nice happens to the Hawks, Stork can't enjoy it because he's too busy waiting for the punchline. Unfortunately, since Merbians developed their pessimism as a survival mechanism, he has a distressing habit of being right.
* TV viewers of a certain age may (vaguely) remember Creator/HannaBarbera's ''LippyTheLionAndHardyHarHar''; the latter was a "laughing" hyena who never smiled, much less laughed.
** Another Hanna-Barbera property the same era was a 1968 reworking of ''Literature/GulliversTravels''. One of the Lilliputians who hung out with the hero was a hard-core Eeyore named Glum; for many, he was the most memorable thing about the show.
** [[CatchPhrase "We're dooooommmmmed."]] (Note lack of exclamation mark. That's just the way Glum rolls.)
* Misery from ''WesternAnimation/RubyGloom''.
* Meg from ''WesternAnimation/FamilyGuy''.
** She veers strongly into TheWoobie category, depending on how the writer treats her. There's a [[FaceHeelTurn post cancellation episode]] where Peter acknowledges that she's not as bad as she deserves to be treated and promises to be nice to her... when nobody else is around. When Lois then enters, Peter proceeds to throw his drink in her face to keep in character.
*** It's worth noting that she occasionally borders on JerkassWoobie; since everyone treats her like crap regardless, she eventually goes on a OnlySaneMan rant about how she is essentially [[TheScapegoat a victim]] of [[WhamEpisode a variety of domestic abuse]] as everybody downright hates her for no reason. However, she reverts back to the Eeyore role ''again'' when she realizes being the Eeyore is pretty much keeping the family together.
* Mentioned under Literature, there's Eeyore from ''Literature/WinnieThePooh''. [[TearJerker Not that anybody cares about ol' Eeyore.]]
** Ironically, during one episode of the cartoon series, the entire cast notices Eeyore, just sitting on a hill and staring and try to cheer him up. The episode ends with Piglet asking Eeyore why he was so sad while Eeyore is still sitting on the hilltop, watching a beautiful sunset, and Eeyore simply asks "What makes you think I'm sad?"
* ''WesternAnimation/ToyStory3'': Chuckles the Clown is a subversion: [[{{Expy}} he acts much like Eeyore]], [[HeyItsThatVoice to the point of sharing a voice actor]], but then you hear his [[StartOfDarkness backstory]].
* A lot of Franchise/{{Transformers}} have elements of the SourSupporter or being real complainers, but Dead End of the Stunticons is known for almost never smiling (lack of a mouth aside) and constantly believing and declaring that their collective doom was at hand. The end result is a character who handily combines fatalism, vanity, and the ability to merge with [[TeethClenchedTeamwork four other belligerent, hardly-cooperative maniacs]] into [[CombiningMecha one particularly large maniac.]]
* ''WesternAnimation/MyLifeAsATeenageRobot'': Jenny's "sister" XJ-7, who has a built-in slouch and frowning expression.
* Moe from ''TheSimpsons''.
* Mr. Ten from ''WesternAnimation/JimmyTwoShoes'' is eternally depressed. It's eventually revealed that [[spoiler:it's Heloise's fault]].
* Tina from ''WesternAnimation/BobsBurgers'' always has a sad look on her face.
* King Malbert from ''WesternAnimation/{{Igor}}''.
* Bad Bill from ''WesternAnimation/{{Rango}}''.
* Stan from ''WesternAnimation/SouthPark'' [[http://www.southparkstudios.com/clips/388734/i-have-cynicism becomes clinically cynical on his 10th birthday]], eventually repulsing his friends.
* Mr. Blossom of ''WesternAnimation/TheDreamstone'' is about the one resident of the Land of Dreams with a hint of cynicism, so much he seems to hold the entire village's worth.
* Bill Dauterive from ''WesternAnimation/KingOfTheHill'', who fell from being a handsome star linebacker to being a fat, balding, divorced sad sack. In particular the Christmas episode "Pretty Pretty Dresses" where he's fixating on his divorce and spends the first half of the episode reminding everyone of their own failed relationships.
* Shady from ''WesternAnimation/MyLittlePonyAndFriends'', not helped by the fact that she was apparently BornUnlucky.
* In the ''WesternAnimation/MyLittlePonyFriendshipIsMagic'' episode "A Friend in Deed", Pinkie Pie is shocked when a new inhabitant moving to Ponyville, Cranky Doodle Donkey, is such a humourless, unhappy DeadpanSnarker that, unlike absolutely everyone else, he doesn't like her and doesn't want to be her friend. She spends the rest of the episode trying to defy this trope, which only makes things worse until she figures out the reason for his melancholy. [[spoiler:He's spent years searching for his true love, whom he lost touch with due to a misunderstanding, and become more and more depressed until he's finally given up and decided to retire to Ponyville with his memories... But it just so happens that she lives there as well, and Pinkie Pie, of course, knows her. Needless to say, Cranky is cured of being The Eeyore by the end.]]
* Fans have viewed Mr. Grumpy from ''WesternAnimation/TheMrMenShow'' as this as well.
* Karl Pilkington from ''TheRickyGervaisShow''.
* Penny from ''WesternAnimation/TheMightyB''.
* Eric Needles from ''WesternAnimation/{{Sidekick}}''.
* Hubert "Dad" Test from ''WesternAnimation/JohnnyTest''.
* Squidward Tentacles from ''WesternAnimation/SpongeBobSquarePants''.
* Mr. Wilter from ''WesternAnimation/ChalkZone'' is always moody to his own students. Not to mention he runs a SuckySchool, no wonder why he's miserable.
* Gordon the Big Engine from ''WesternAnimation/ThomasAndFriends''
* Lofty from ''WesternAnimation/BobTheBuilder''
* [[BigBad Nester]] and his mom from ''WesternAnimation/ScaredySquirrel'' are both pretty depressed. Mainly because of Scaredy.
* Bad Luck Schleprock from ''ThePebblesAndBammBammShow'', a spinnoff of TheFlintstones. Wowzy wowzy woo woo.
* PJ on ''WesternAnimation/GoofTroop'' oscillates between this and a very compassionate variation of a StepfordSnarker. He ''can'' be happy sometimes, and the fact that he usually isn't is evidence of the injustices done to him, both immediately beforehand and throughout his entire life. He's somewhat better in the first movie, and in the second movie, [[EarnYourHappyEnding his unhappiness is finally rectified]].
* Downer from ''WesternAnimation/TheBiskitts'' fits this trope.
* Kevin the bulldog from ''WesternAnimation/MrBogus'', most of the time.
* Reggie from ''WesternAnimation/FreeBirds''.
* The Warden from ''WesternAnimation/{{Megamind}}''.
* Bunk from ''WesternAnimation/{{Poochini}}''.
* Sal, a minor character in ''WesternAnimation/{{Detentionaire}}'' who only shows up twice, first giving the tour of the Green Apple Splat factory and then dressed as a chicken working for the restaurant food chain Big Chicken. With a life like that, one can kind of see why he only talks with a tired monotone.
-->'''Sal:''' Now if you all come with me we can all watch a wonderful film about your favourite drink. [[DullSurprise Hooray]]. [Later] And that's the movie. Wow. Every time I see it I wonder why I get out of bed in the morning.
----