* The dogs howling during the dog pound sequence.
* Trusty's [[spoiler: DisneyDeath]]. Jock whimpering, then when he nudges Trusty and there's no response.
* Lady's TheReasonYouSuckSpeech to Tramp doubles as a CrowningMomentOfAwesome, but you can hear the sadness in her voice.
** Makes it doubly more saddening when Tramp leaves (because Lady tells him to) and you can see from his expression that he really fell in love with her.
* Lady's sad and confused reaction after Darling ''hit'' her. It wasn't anywhere close to animal abuse, but you can't help but feel pity for the poor girl.