* The end of Pinkie's Brew, to some.
-->''"Someday I'm gonna go home... *sigh*"''
* [[spoiler: Raincloud's [[HeroicSacrifice suicidal rescue]] of the Mane 6]] at the end of ''Cherry Bomb'' is heartbreaking, especially for [[ComedicSociopathy a series like this.]] Made worse by the fact that [[spoiler: everypony treated her like garbage and didn't give two horseapples when she died.]]
** Made ''doubly'' worse by the fact that Pones made the very intentional choice to use the music clip that plays when [[WesternAnimation/TheIronGiant the Iron Giant]] makes his own HeroicSacrifice.
** ''Triply'' when you learn [[spoiler: Raincloud was the leader of the town's Rescue Squad. Now that she's gone, her beloved people have no defense against all the mayhem caused by the mane 6.]]
* Sweetiebot's 'If you don't love then you're going to fear me' speech.
* The fate of Cadence in 'Foaly Matripony'. Francis (her fiance) decides to marry Twilight, who keeps her trapped in a cave. During the credits, [[spoiler: CADENCE DIES]].
** The dark implication that Francis was a victim as well: Twilight may have brainwashed him into loving her and becoming one-dimensional with no higher brain function that a surfer dude.
-->'''Francis:''' ...and yet I don't feel anything!
-->'''Twilight:''' And you never will, ever again...
* The way Spike is treated by the Mane Six, especially Twilight. It is also likely that when he grows up he'll be caged and have stuff thrown at him.
* The fact that Rarity apparently designed a sweater that was specifically invented to give her hugs. The war must have ''really'' messed her up.