* [[spoiler:Kagura]]'s death in Season 3.
* Rei Kakizaki's [[BreakTheCutie entire backstory]] from the start of the ZombieApocalypse up to Season 3.5 could easily qualify, but special mention goes to her reaction to [[spoiler:Ran's death. Unable to cope with the sudden loss of her one remaining sister and Living Emotional Crutch, she breaks down completely, quietly sobbing and pleading for her to Please Wake Up.]] Made even worse by the knowledge that she really did believe that [[spoiler:Ran]] had been the only person left in the world who loved and cared about her, and that now she was completely, utterly alone.
** And speaking of Rei, in the Discussion Threads, there are occasionally pictures of the entire cast as analyzed by certain cast members. [[http://gondopedia.net/Yukeswiki/images/e/e2/Mute.jpg This one]], done by Rei, has a rather heartbreaking moment when she gets to Kagura's Mother's picture: [[spoiler:"Are Mom and Dad and Ran and Misato angels now too?"]]
* While it counts as a CrowningMomentOfAwesome, [[spoiler:Colonel Jenkins's HeroicSacrifice]] would totally count as this back in Season 2.