[[folder: The show ]]
* Despite being treated as a joke villain (like everyone else) what happened to Doctor Two-Brains is pretty sad. An intellectual NiceGuy driven to evil and insanity by placing something on his head, voiced by Creator/TomKenny? Two-Brains is basically a science-y [[WesternAnimation/AdventureTime Ice King]] on a show with a younger target audience.
* Violet [[spoiler:finds out her best friend Becky is [=WordGirl=],]] and felt betrayed for being lied to. She tells Becky she's not sure if they'll be friends anymore, making Becky cry.
* Reason leaving Rhyme behind after getting fed up with her incompetent attempts at villainy. He doesn't just want to leave their criminal partnership, he wants to end their ''friendship'', and he returns his half of their friendship necklace to her when doing so.
* A show-wide one that isn't dwelt on but is still present: Becky has all of the traits of [=WordGirl=], since she ''is'' [=WordGirl=], but no one ever gives her proper credit for them since she's just a normal girl. Is it any wonder she's so shocked to hear Tobey praising her in "By Jove, You've Wrecked My Robot"?
[[folder: The Rise of Miss Power ]]
* This happens in "[[BigDamnMovie The Rise of Miss Power]]" when [=WordGirl=]/Becky Botsford insulted her sidekick Captain Huggyface/Bob. Though she later apologizes to him, this still counts as a TearJerker.
** [=WordGirl=] is [[NoHoldsBarredBeatdown beaten]] by Miss Power, and is given TheReasonYouSuckSpeech, and is called weak, and even has her star insignia removed.
** Earlier it hurt a lot to see [=WordGirl=] mocking Tobey who is often somewhat of an FriendlyEnemy and reducing him to an inability to move out of despair and a few others. Not to mention the Butcher's frantic and confused pleading to know where [=WordGirl=] is when Miss Power is taunting him, saying that [=WordGirl=] isn't mean like Miss Power.