* The Moonwatcher's tribe are quite [[TheWoobie woobies]] before the Monolith gifted them intelligence: pathetically looking for scraps of food as they co-exist with tapirs that aren't scared of them, being easy snacks for leopards and getting driven away from their water pond by a smaller but nastier tribe of ape-men. The establishing shot of the night scene before the Monolith's arrival shows a rock formation with a cave entrance, only to then cut to a growling leopard not too far away and reveal that the ape-men are actually trying to sleep in a much more vulnerable spot outside: the implication is that the feline scared them out of their own home as well. What an awful day.
* Dave releasing Frank's body out into space.
* The murder of the hibernating astronauts. Despite doing nothing to Hal, they become victims of his madness.
* Amazingly, HAL's VillainousBreakdown and death scene. After all of his murders, [[AlasPoorVillain his helpless pleading for Dave to stop pulling the plug on him will make you wish there was another way.]]
** [[https://www.youtube.com/watch?v=c8N72t7aScY Now with video]]. "Stop, Dave. I'm afraid." and of course, the famous portion at 3:48 - "Daisy, Daisy... give me you-r... answer... do."
** After the discussion of [=HAL's=] malfunction, his having killed the rest of the crew, and having heard his calm-in-a-creepy-as-hell-way voice for the whole movie, this WhamLine points out that, somewhere deep inside, he's as human as any of us and just as afraid to die. To paraphrase a Website/YouTube comment about HAL's death: "It's like watching the execution of an invalid."
** "I'd like to hear it, HAL. Play it for me." Even after everything that's happened, Bowman still treats HAL like a human member of the crew.
*** [[FridgeHorror Unless he's just trying to keep HAL talking to make sure when he's dead.]]
** This gets even worse when you read the novel (or read/watch ''2010''): HAL's psychotic behavior [[TragicVillain wasn't even his fault]]. He was told to conceal the existence of the monolith, which directly conflicted with his programming. This is what caused him to mistakenly report the communications relay was broken, and what ultimately resulted in him deciding to kill the crew; if there was no one to keep the secret from, he wouldn't have to keep the secret. Thus the conflict will have been solved.