* "Pictures In My Head." Kermit's sense of nostalgia and loss is conveyed extremely well.
-->'''Kermit:''' I mean the only way we can raise that kind of money is to....... to put on a show. ...and I haven't seen the old gang... in a long, long time. ... I guess... the world sort of forgot about us."
** It gets especially great when the portraits come to life and The Electric Mayhem starts playing. The lyrics so beautifully capture what was "lost" and how great those memories truly were... and how wonderful it would be to have them back.
-->Should we do it all again?
-->Make them laugh like we did then?
-->Back on stage where we belong
-->We could harmonize for one more song
** It gets especially heartbreaking when Kermit gets a glimpse of Miss Piggy's portrait and sadly covers it again.
** Rarely can the Muppets invoke such a strong sense of ''guilt'' as "Pictures In My Head", since in a meta sense, the audience is responsible for the Muppets losing popularity.
* The moment when Walter, Gary, and Mary are trying to talk Kermit into doing the telethon. They each quote a line of the Muppet Show theme song . . . and then Kermit plays the final 7 notes very slowly and regretfully on the piano.
* Kermit singing "The Rainbow Connection" and being joined by Piggy and then the rest of the cast. (And the in-movie audience. And probably more than a few of the real audience.)
** When Animal starts drumming again, you will cry.
*** Add to that the fact that this is the first time Steve Whitmire has gotten to perform Kermit's signature song since taking over the roll.
* Watching Tex Richman [[spoiler: harassing the Muppets after the telethon failed. The way he gets in Kermit's face always made me want to punch the guy.]] Even the in-movie audience was appalled by his despicable behavior.
* The scene where Kermit makes his uplifting speech about [[spoiler: so what if the telethon didn't raise enough to buy back their studio and their name. They have each other and that's what makes them who they are, so they'll walk out with heads held high, and they'll start over from the beginning if they have to. As soon as Kermit opens the stage door, he's greeted by camera flashes and cheers. The shot pulls back to reveal Hollywood Boulevard is absolutely packed with screaming fans waving signs and cheering the Muppets.]]
* When Walter first enters Kermit's old office, he sees on one wall an assortment of photos of Kermit with old celebrities, the center of which is... Kermit with JimHenson.
* Just seeing what's happened to Fozzie without Kermit and the others is absolutely heartbreaking.
* At the very beginning of the movie, this troper lost it when Gary's friends made fun of Walter's Kermit Halloween costume. That scene describes [[TruthInTelevision just about every teenaged Muppet fan since 1990's teen years absolutely perfectly... a bit too perfectly]].
** Hell, it describes anyone being made fun of for being a fan of anything too perfectly.
* RealLife example: Creator/JasonSegel visibly tearing up when Bret [=McKenzie=] thanked Jim Henson after winning an Oscar for best original song.
* Seeing the pictures of Jim Henson and knowing that he would like this movie if he was still alive.
* The "Man or Muppet" number has a bittersweet ending in which Gary takes a bus all the way back to Mary's house and apologizes to her, then the two of them embrace.
* Jerry Nelson's very brief vocal appearance is a TearJerker in light of his August 2012 passing, since this was the final Muppet movie he lived to see and be involved in.