* The aftermath of [[DeathByOriginStory Uncle Ben's death]] really hits hard in this movie, especially the scene directly following when all Peter can do is listen to the voicemail his uncle left him -- and he can't even do that without [[HeroicBSOD breaking down.]] Hell, even ''[[JerkJock Flash]]'' comes to Peter at school [[HiddenHeartOfGold and apologizes for being an ass.]] Jeez, it's like [[SandInMyEyes glass shards to the eyes...]]
* Gwen showing up at Peter's house after [[spoiler: her father's funeral]] and desperately asking him why he wasn't there.
* [[AdultFear Everything]] Aunt May goes through, her family slowly being ripped apart first with the Parkers disappearing, then her husband being shot, and her nephew that she raised coming home late at night covered in cuts and bruises. [[spoiler: And then at the end of film, he comes home covered in blood and she just holds him tightly.]]