* Chancellor Gorkon's death is surprisingly tearjerking, [[spoiler:especially when you consider people in ''both'' Starfleet ''and'' the Klingon Empire worked TOGETHER in order to assassinate him. Because they didn't want him to make an alliance between their two governments!]]
** His last words are heartbreaking: "Don't let it end this way, Captain..." Kirk, who expressed bigotry towards the Klingons in his own personal log due to [[Film/StarTrekIIITheSearchForSpock (three of) them murdering his son]], stands there shocked with cognitive dissonance: instead of cursing him in his last breath, the man was begging Kirk to save the peace process. Kirk observed bitterly later that "Gorkon had to die before I realized how prejudiced I was", and regretted not taking him at his word from the getgo.
* "Second star to the right...And straight on 'til morning." ManlyTears were shed.
** After ''Film/StarTrekGenerations'' was released, ''VI'' became doubly poignant because fans realized it really ''was'' the last time the crew would adventure together. Then there's the "Sign Off."
* Merritt Butrick's cameo - the film was ''after'' he had died of AIDS complications. In-universe, Kirk unable to reconcile his death.
* More like a Fridge Tearjerker, but how Valeris' betrayal affects Spock. She's second only to Saavik when it comes to Spock's proteges. He tried his best to teach her how to have hope, but she wasn't good enough to live up to it. She didn't just betray him as a Starfleet officer, she didn't just betray him as a Vulcan, she betrayed him [[LikeASonToMe as a surrogate daughter]]. That's a hard blow for anybody.
** Her betrayal gets him [[OOCIsSeriousBusiness openly angry, causing him to smack the phaser out of her hand]]. [[BerserkButton Meaning that he's angry enough for it to crack that Vulcan calm]]. Oh yeah, he is PISSED, and it is tragic, because it means that he trusted her that much.
** And then he's still horrified after being forced to [[MindRape dig the conspirators' identities out of her mind]]. These sequences feature some of Creator/LeonardNimoy's best acting in the whole franchise.
** Imagine how heartrending it would have been had it ''been'' Saavik, [[WhatCouldHaveBeen as Meyer wanted]].
* The fact that among the conspirators was [[spoiler:Admiral Cartright]], a relatively minor (though friendly) character who had previously appeared in one of the more popular ''[[Film/StarTrekIVTheVoyageHome Star Trek]]'' films may come as a slap in the face to some fans.
* Kirk's reaction to finding out that Spock felt the need to vouch for him over the mission to escort Chancellor Gorkon to Earth. He's visibly angered and disturbed that Spock thought he wouldn't follow his orders to the best of his abilities whatever his personal feelings might be.
-->"You should have trusted me."
* The look on Spock's face when Kirk retorts "let them die!" To see the normally stoic Vulcan shocked and horrified at his friends words truly underscores the callousness and bigotry of Jim's statement