Given that Slayers is such a comedic show, you never thought it would have any of these, did you. Well it is the case, just as there were [[CrowningMomentOfFunny funny moments]] in ''Anime/NeonGenesisEvangelion''.
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* In ''{{Slayers}} NEXT'', it's almost impossible to not feel bad for little Amelia [[spoiler: when she thinks her dad is dead (he survived the actual assassination attempt and then just faked his death to uncover the plotters, but she doesn't know that yet) and breaks down crying in Phil's library as she finds the book that her dad used to read to her when she was a little girl]]. That one was so sad that even ''Lina'' got briefly depressed and gave her a hug before promising to help her through all of this.
* When Amelia [[spoiler: is nearly killed by Gaav, and later temporarily IS killed by Hellmaster.]] Zelgadis' reactions, both times, make them extra painful.
** [[spoiler: ALL the temporary deaths that Hellmaster inflicts on the cast sans Lina qualify. Aside from Amelia, the one that probably stings most is Martina. Yes, '''Martina''', the freaking ''comic relief character'', wasn't even spared a gut-wrenching temporary death.]]
* In ''TRY'', when [[spoiler: Valgaav dies at the end]]. Even though he's [[spoiler: reborn]], the only character who isn't depressed by this is, of course, Xellos.
* In ''{{Slayers}} Next'', right after [[spoiler:Lina defeats Phibrizzo, and everyone is waking up only to find that "Lina" does not exist anymore.]] The way that [[spoiler:Gourry]] stands there watching her and then says "I don't know what's going on- but you are taking [[spoiler:Lina]] away, I want it undone! I WANT IT UNDONE!" And everyone joining in to tell off the ''Lord of Nightmares''.
* In episode seven of ''NEXT'', a chef tells Amelia that he wants to help her and her friends catch the dragon and make dragon cuisine because he was unable to make some for his late granddaughter, [[spoiler:who looks like Amelia.]] It turns out that he told every member of the group the same story, [[spoiler:telling Lina that she looked like his dead daughter, that Gourry looked like his dead son-in-law, and that poor Zelgadis looked ''like his wife''.]] At first it seems like it's a false tearjerker meant to get a laugh, [[spoiler:then we find out at the end that he was telling the truth, due to him having a locket with a picture of his family.]] If you watch that scene again and now know [[spoiler: he's telling the truth]], you may be inclined to tear right back up, especially with the music playing during the scene.
* [[spoiler: Gaav]]'s KickTheDog moment in which [[spoiler: he verbally brutalizes Amelia, then tries to kill her... and Zelgadis goes TakingTheBullet to protect her, which causes the poor girl an HeroicBSOD as he bleeds to almost death in her arms.]] That was so freaking ''sad''.
* The opening to the 23rd episode of ''NEXT''. [[spoiler: Gaav's dead, but Phibrizzo has revealed himself and taken Gourry away with him.]] Lina wakes up, remembers what happened the previous day and the reality hits her that [[spoiler: Gourry]] is gone. She then notices that there's something falling on her lap and realizes it's her own tears; the whole time she's started crying. And then immediately after that she collapses into full blown sobs.
* Episode 10 of ''Evolution-R'', when we discover from Rezo that [[spoiler:it is impossible for Zelgadis to restore his body]]. Zelgadis' agony is heartfelt and sells in ''both'' languages...he even ''cries'' for the very first time in ''any'' form of media!
** [[spoiler:Of course, this kind of lessens when you realize that [[WordOfGod Hajime Kanzaka]] stated that Zelgadis ''will'' truly find his cure one day. Then again, considering that [[WeAreAsMayflies he will live longer because he's a chimera,]]...]]
* In ''Revolution'', when Pokota can't bring himself to execute Duclis.
-->'''Duclis''', ''affectionately'': [[CrowningMomentOfHeartwarming You really are a hopeless prince]].
* [[spoiler:Milina's death]] in the Light Novel. [[spoiler:During a bodyguarding job she is stabbed with a poisoned knife. [[KickThemWhileTheyAreDown She is denied medical care from one man and the other does not know the spell needed to heal the toxins]], and Milina dies as a result, cuing [[WoobieDestroyerOfWorlds Luke]]'s RoaringRampageOfRevenge and [[FromBadToWorse Shabranigdo's awakening]].]]