!!If you're looking for examples relating to a specific show or movie, see these designated pages:
* ''Tearjerker/ScoobyDooMysteryIncorporated''
* ''Tearjerker/ScoobyDooDirectToVideoFilmSeries''
** ''Tearjerker/ScoobyDooOnZombieIsland''
** ''Tearjerker/ScoobyDooInWheresMyMummy''
** ''TearJerker/ScoobyDooMoonMonsterMadness''

* The conclusion of ''Scooby Doo and the Alien Invasion'', when Shaggy and Scooby say their goodbyes to Crystal and Amber.
-->'''Velma:''' When I said there was someone out there for you, I didn't think...(''looking towards the sky'') out ''there''.
** When asked if they're alright with Crystal and Amber leaving, Shaggy, in the most defeated voice he's ever spoken with, says "Yeah... we're just completely, totally destroyed."
** Suddenly subverted when Velma comes up with a box of Scooby Snacks for them. (The sight of Velma with her glasses askew and her hair mussed up during the tussle for the box is priceless.)
--->'''Velma:''' Well, ''that'' didn't take long!
** Though that may actually have been Shaggy and Scooby trying to cover up how much they're still hurting (combined with HeartbreakAndIceCream). [[SarcasmMode Ain't love fun?]]
* In ''Music Of The Vampire,'' Shaggy thinks he's been bitten by a vampire (he was actually grazed by an errant stake) and seeks refuge in the swamp. He tells Scooby to let him go it alone for fear of turning into a vampire, and this potential split up is heart-tugging. Shaggy can't do it, as he and Scooby suddenly reunite.
* In "Scooby Doo and the Mummy Too" from [[WesternAnimation/ScoobyDooWhereAreYou the original series]], Shaggy is pretty upset when he thinks Scooby's been [[TakenForGranite turned to stone]], as is Scooby when he finds out...."[[NotNowWereTooBusyCryingOverYou Look Scoob, you've been turned to stone!]]" ''(Both proceed to cry)''
* In the Live Action movie, the entire gang splits after Velma quits, leaving only Shaggy and Scooby. Shaggy drives the Mystery Machine away while Scooby looks out the back window for the others, heartbroken.
--->'''Shaggy''': Looks like it's just you and me now, Scoob.
* A meta example is the treatment the franchise gives [[TheScrappy Scrappy Doo]]. He was created as a way to appeal to the younger audiences, but the result ended up backfiring and nearly ''everyone'' hates him. It doesn't help that CartoonNetwork and {{Warner Bros}} continues to take jabs and jabs at him, despite having a rather small, but strong fanbase. To put the hatred Scrappy gets is...[[FanDumb an understatement]].
** The ''Worst'' part about the above? Scrappy-Doo was created to save the show from cancellation in the seventies. Despite the backlash, he actually managed to give the show the boost it needed to stay afloat. He saved the franchise, and ''this'' what he gets in return.