* No love for the death of the faithful [[spoiler:CompanionCube]]?
** The worst part? In ''VideoGame/{{Portal 2}}'', [[spoiler:[=GLaDOS=] says the companion cubes are ''sentient.'' This is right after she vaporizes two or three cubes in a single test chamber.]]
** In Test Chamber 7 of the sequel, you run into another Companion Cube. At the end of the chamber, [=GLaDOS=] tells you that while the Emancipation Grill would normally eliminate anything you try to take with you, this one is broken, so don't take anything. So you go to get it back, take it down to the elevator and...
--->'''[=GLaDOS=]:''' I think that one was about to say 'I love you.' They ARE sentient, of course. We just have a LOT of them.
** The Companion Cube poetry behind the walls of level 17. Poor Rattman...
* The first time you knock over a turret, only to have it say, in a melancholy voice, "I don't hate you..."
** If you use a turret as a shield while a different one is firing at you, your makeshift shield screams, [[VideoGameCrueltyPotential "W-wait! No! ...It's me!"]]
** The turret voices have been updated in Portal 2, with a heartrending "Nooooooo!" if you fling them into space. [[spoiler: Thankfully, the turrets forgive you in the end and sing you a song.]]
** The good turrets being thrown into the Redemption Center after correctly responding to the template.
--> '''Turret:''' But...why? I did everything you asked! '''''*CRUNCH*'''''
* The Curiosity Core is a childish, naive little AI that asks questions about anything it sees and cheerfully greets you once it recognizes you, all in a cutesy voice that sounds like the turrets but absent the creepiness. [[spoiler:It's also the first core you incinerate. It's scream is also pretty horrible to listen to.]]
* '"Look, we're both stuck in this place. I'll use lasers to inscribe a line down the centre of the facility! And one half will be where you live, and I'll live in the other half. We won't have to try to kill each other, or even talk if we don't feel like it!"' [[=GLaDOS=]] actually sounded a bit freaked out here...or as much as a computer/robot/AI thing could.
** In the sequel its revealed early on that [=GLaDOS=] spent the time since the first game being forced to relive Chell killing her over and over. And that's not even counting the crap she goes through later. Combined with the fact that Valve went out of their way to make her more sympathetic as the game goes on... [[spoiler:Fortunately, she gets a relatively happy ending.]]
* Finding out that [[spoiler:Chell gets dragged back into the lab again]] was depressing enough, but hearing that in Portal 2 she wakes up ''centuries in the future'', Jesus, there is literally nothing left for her outside. The world outside the lab is a completely different place by now, and she can't even have the TragicDream of going back to a normal life anymore. She doesn't have anything worth fighting for anymore besides her own life.
** Not only that, but as revealed in the comic, [[spoiler:Doug Rattmann attempted to save Chell from getting dragged back into the labs, ignoring his spirit Companion Cube telling him to run, insisting that he had to try to save her. Seeing that the Party Escort Bot had put her in an offline cryo-stasis chamber, Chell would have died if it hadn't been for Doug activating the chamber, even though he knew that he couldn't set an automatic wake-up time. Without the intervention of Wheatley, she would ''never'' have woken up.]] TearJerker for Doug and Chell indeed.
** Rattmann's dialogue during this part is pretty sad as well.
--> '''Rattmann''': So it's the long sleep...or the long sleep. And I don't know which is worse. Forgive me.
* Towards the end of Lab Rat, the Companion Cube is asking Doug how he knew that Chell would be able to do it, how he knew she could get the two of them out. He gives a perfect non-answer, saying that he just had a hunch. It's not until after he dies that we learn why he chose her.
-->'''Proctor's Note:''' Test subject is abnormally stubborn. She never gives up. Ever. Rejected, do not test.
* The end of Chapter 5, if you think about it. You're so close to achieving your goal, [[spoiler:Wheatley is getting you out at long last,]] only for the triumphant music to suddenly take a dark turn... and you watch as everything starts to go terribly wrong and [[spoiler:Wheatley, your one friend]] starts to lose it[[spoiler:, mad with power and suddenly turning on you, claiming your work was his]]. The worst bit? When the music swells again, you can hear nearly the exact same victorious music from merely a MINUTE ago, when you had seemingly won, but now it serves as a [[DarkReprise Dark Reprise]] of what you almost had together. [[spoiler:Why, Wheatley? Why?...]]
* Even after hearing all the hilarious craziness of Cave Johnson in the sequel, you'll be sad by the end. Cave Johnson made some amazing things and had absolutely no morals whatsoever, but...everything he created, he did by accident. He wanted to make diet pudding; he made repulsion gel instead. He wanted to make a fuel injection system de-icer; he got a psychotic AI instead. This is all very funny...until then you find out [[spoiler:he's dying from exposure to all the crazy things he's experimented on, and despite the amazing devices his company has created, by the end they can't even afford to pay the homeless and unemployed a few bucks to act as test subjects. Even his plan to save himself by uploading himself to a computer never works out...for him, anyway]].
** The real moment is just at the end of his rant. [[spoiler:Even though it sounds mostly in [=GLaDOS=]' usual monotone, something about this moment...]]
---> [[spoiler:[[foldercontrol]]

[[folder: [=G La DOS=]: Goodbye, sir. ]]
]]
** What makes the above (including the farewell) even more sobering is when you stop and actually realize that these recordings are all that's left of Cave Johnson, and that they'll almost certainly never be heard again. [[spoiler: They, along with his image and the parts of the facility he oversaw himself, will now sit underneath Aperture Laboratories forgotten, in silence, forever]].
*** "Haec omnia transeunt" [[spoiler: All things pass away]]
** [[HopeSpot It's implied in the Co-op mode, however, that]] [[spoiler: [=GLaDOS=] eventually got to link herself with the old facility's power grid, which means that she, along with her new batch of test subjects, hasn't seen and heard the last of Cave Johnson]].
** His undeniably awesome combustible lemon rant is quite sad when visualizing what he must have looked like when recorded it. [[spoiler:A near penniless man overlooking his empire as it stands on its last legs while slowly dying a painful death, hunched over a tape deck after he just downed another round of pain pills, screaming his frustration at life while tears stream down his face, most likely breaking down into sobbing after the tape stops recording. Even sadder is that his misery is almost entirely his fault to begin with, and his screaming rant is likely an attempt to deflect his own personal responsibility.]]
** The recording you hear before the [[spoiler: Conversion Gel]] chamber.[[spoiler: "Welcome to the Enrichment Center...*cough*"]] At this point, [[spoiler: with just that one line of dialogue, you already know everything about Cave's situation. He's broke. He's sick. He's completely demoralized. The enthusiasm of a once magnificent entrepreneur and one of the greatest minds of a generation, gone. All that remains is science, and so, almost as soulless as the robots installed to replace his management, Cave greets you with a simple line...and yet the way he says it, all the subtext of a defeated man...]]
* [[spoiler:Wheatley floating through space]] in the ending of Portal 2. Especially because [[spoiler:he's genuinely sorry for what he did, but in all likelihood, he'll never be able to make it up to Chell]].
** Even moreso when you remember that [[spoiler:he honestly couldn't control himself when he was attached to the body. The fact that he remembers it means he knows he did it, and he probably didn't want to hurt Chell at all.]]
** Another part is when Wheatley screams [[spoiler:"GRAB ME, GRAB ME!" Given just shortly before in the boss dialogue, he revealed how hurt he was that you never caught him, it just twisted the knife.]]
** For a bit of horror mixed in, remember that this IS the ''VideoGame/HalfLife'' universe, and there are very likely to be mind-breakingly horrible things [[spoiler: in space.]]
* At the end of ''Portal 2'', [[spoiler: when [=GLaDOS=] says she's letting Chell go and deleting Caroline. She could've killed Chell and deleted Caroline easily while you were knocked out, but she didn't. It's implied by her actions and "Want You Gone" that she lied about deleting Caroline to intimidate Chell into never coming back, probably because [=GLaDOS=] is worried she'll go insane again.]]
** In typical [=GLaDOS=] fashion, she contradicts herself repeatedly in the ending song, first admitting again that you were her "only friend", then denying that she was talking to you, then erasing the words [[spoiler:"Maybe I'll stop feeling so bad"]] from the screen even though she still sings them. You're watching her struggle with complex and conflicting emotions towards someone who's become so important to her, first as an enemy and then as an ally and then as a friend, and she is struggling with it even as she desperately pushes you away and denies that "She needs anyone".
** What's more, in another tear-jerking occasion at the end and also possibly a contradiction, [[spoiler: the turrets singing the "Cara Mia" opera at the end. Translated, the lyrics are a final farewell to Chell as she ascends to the surface, possibly organized by the Caroline part of [=GLaDOS=].]] Personally, it was perhaps the most emotionally affecting part of the whole ending ([[spoiler:and not because of the beautiful soprano]]).
** Of course, as she herself states [[spoiler:"Killing you hasn't worked out all that well in the past."]]
*** This is after she admits that you weren't her greatest enemy, but her best friend. The ensuing MoodWhiplash is what makes it so sad.
** Note too that a few scenes before, [=GLaDOS=] mentions [[spoiler:that she's hearing a conscience and it's her own voice. Where as before, she may have been conflicted between a handful of negative emotions, now [=GLaDOS=] has to deal with more positive ones that outright contradict everything else she's thinking. In short, she now has a Wheatley she can't ever get rid of and one that's much more powerful than the actual Wheatley.]]
* Right before the above example, when [[spoiler:Chell wakes up after the complete and utter Lunacy]]:
-->'''[=GLaDOS=]''': (genuinely) [[spoiler:Oh, thank God! You're all right!]]
* When you wander into the Bring Your Daughter to Work Day Science Fair in Portal 2. [[spoiler: There are names on all of the projects, and on the very last project, in small grade-schooler scrawl, is written 'Chell'. This has led to theries that she was a daughter of scientists working there, and they were most likely killed by [=GLaDOS=].]]
* In regards to whether [=GLaDOS=] [[spoiler:deleted Caroline]] or not, WordOfGod confirms [[spoiler:[[http://www.pcgamer.com/2011/04/26/erik-wolpaw-on-portal-2s-ending-the-spoiler-is-probably-lurking-out-there-somewhere/ she did]].]] Except for ''now'' WordOfGod says that [[spoiler:[[http://www.fastcompany.com/1750003/portal-2-valve-eric-wolpaw-jeep-barnett-doug-lombardi she only ''might'' have deleted Caroline.]]]] Oh Valve and your ability to change your mind that leaves things up for speculation! [[spoiler:So Caroline might not be deleted after all!]]
* And just to twist in the knife, here's the translation of [[spoiler:lyrics to the opera the turrets sing to you as you leave:]]
-->[[spoiler:Dear beautiful, my beautiful darling]]\\
[[spoiler:My child, oh Chell [pun: "oh heavens"] ]]\\
[[spoiler:For I hold her in esteem]]\\
[[spoiler:For I hold her in esteem]]\\
[[spoiler:Farewell to my dear!]]\\
\\
[[spoiler:My dear girl,]]\\
[[spoiler:why do you not walk away]]\\
[[spoiler:Yes, far away from science]]\\
[[spoiler:My dear, my dear girl]]\\
[[spoiler:Ah, my beautiful]]\\
[[spoiler:Ah, my dear]]\\
[[spoiler:Ah, my dear]]\\
[[spoiler:Ah, my child]]\\
[[spoiler:Oh dear, my dear]]
** "Cara Mia" is at the top of this list for some players, as the translation shows it's essentially [[spoiler:an aria sung by a mother saying goodbye to her child]]. Take that with some of the LukeIAmYourFather theories and it becomes downright heartbreaking: [[spoiler:if you think of [=GlaDOS=] as Chell's mother (or something that ''[[BodyHorror used to be]]'' her,) then the song is basically [=GlaDOS=] announcing to Chell that she loves her and she's sending her away to protect her. "Stay away from science..."]]
*** Well, some of her words have an interesting twist when you think she loves Chell:
---->One day they woke me up, So I could live forever, Itís such a shame the same will never happen to you. ([=GLaDOS=] is genuinely sad she will outlive Chell?)
---->Youíve got your short, sad life left, thatís what Iím counting on, Iíll let you get right to it... ([=GLaDOS=] is saying that life is short, enjoy it now and forget about everything that's happened?)
---->Go make some new disaster, Thatís what Iím counting on, Youíre someone elseís problem... ([=GLaDOS=] wants Chell to ruin someone else's plans - the Combine?)
* [[http://rain.nxe7.com/ Fan content, but beautiful.]]
** [[http://www.youtube.com/watch?v=5v6RFiI7_xU&playnext=1&list=PL498F06D99B95BFB3 This]] fan video, which uses the same song and looks a bit reminiscent of Wall-E's ending credits at times. If you didn't pity Chell before, this will do the trick.
*** https://www.youtube.com/watch?v=XUwkGFw226U&list=FLjr9-jN54l9t7qJf6xORa9A This will break your hearts over and over.
* Playing Chapter 9 right after 5 is absolutely heartwrenching. Watching what was basically your best friend go mad as the rush of power reinforces every negative emotion, then having to take down said friend while he raises some concertingly good points (You DID drop him and you DID fail to mention [=GLaDOS=] would be rather pissed if she ever woke up) is an emotional [[PlayerPunch sucker punch]].
** [[WoobieDestroyerOfWorlds Wheatley's]] VillainousBreakdown during the final boss fight. His TheReasonYouSuckSpeech is less like an villain's evil gloating and more like the ranting of a heartbroken child who doesn't understand why everyone has turned against him, or maybe that of a scorned lover who hates you for choosing someone else. As he starts to lose it more and more, he accuses you of taking advantage of him all along as a means to escape, then abandoning him when he needed you most so that you could run off with your "best friend over there." He even brings up the fact that you didn't catch him when he dislodged from his management rail, which he honestly believed would have killed him, and hysterically accuses you of missing on purpose because you wanted to get rid of him. (This is made much, much worse by the fact that even if you honest-to-god tried to catch him, [[CrueltyIsTheOnlyOption it's impossible to do so.)]] What makes it even worse is that, if you listen closely to his voice during the rant, he sounds an awful lot like he's ''crying''.
** And it's enhanced by the music- if you listen between cores, you can hear a remixed version of 'I AM NOT A MORON' inside the adrenaline-pumping BossBattle Music- his ''victory music'' is playing. Aperture is ''literally'' crashing down around him, and he's about to lose his power- what crueler way to accentuate that than a DarkReprise of his victory tune?
** Although still sad, some of this is tempered a bit by the fact that the only reason you 'abandoned' him was that he went insane, trapped you in an elevator and punched you down an elevator shaft.
* During the final boss fight, [[spoiler:the Aperture Science Handheld Portal Device is the first thing to get sucked out into space.]] Goodbye, old buddy...You served us well.
* Leaving the Oracle Turret behind. Twice.
** When picking up the Oracle Turret for the first time, she just sounds so relieved and sad and exhausted in those two words: "''Thank you...''"
** "[[http://www.youtube.com/watch?v=fKURvGuWOso I'm Different]]".
* When [=GLaDOS=] retorts [[spoiler:"What exactly is wrong with being adopted?", which speaks volumes about her ability to access some of Caroline's memories.]]
** Especially when you contrast it to one of her quotes from the first game: "[Your profile] also says you're adopted, so that's funny."
* [[http://www.youtube.com/watch?v=C4KLv8Iuv7E Why, Wheatley, why?]]
* Exile Vilify just brings tears to eyes. Especially after reading the Lab Rat comic, and learning about Rattman's past.
** The song was apparently originally used for an easter egg ending, presumably one with a [[DownerEnding not so happy feel.]]
** You think the song's sad enough on it's own, right? Well, guess what Someone made a stop-motion music video of the song with Rattman and Chell. It makes the song go from tearjerking to outright '''depressing'''. You can watch it [[http://www.youtube.com/watch?v=XUwkGFw226U here.]]
** [[http://www.youtube.com/watch?v=o9t6zGesLi0&feature=related Here's]] another music video, made specifically for a contest, with Wheatley singing it. Sounds ridiculous, doesn't it? Well, that is until you realize that he's singing it [[spoiler: in space...]]
* Is Caroline's consciousness really stuck inside [=GLaDOS=], alone and unloved, for eternity? That's pretty heartbreaking. %% Speculation on this point does not belong here.
* [[spoiler:[=GLaDOS=] sounds so sad and alone when singing 'Want you Gone'. The strongest part of the song is either how she tries to retract the lines "maybe I'll stop feeling so bad", but still stays them, or the part when [=GlaDOS=] says "I don't need anyone now." The way she sings it is really sad: a bit deeper, and her voice trembles as much as it's possible for a robot. It sounds as she's forcing herself to say it and/or is in the verge of crying.]]
* In Co-Op mode, one of the voice-overs [=GLaDOS=] is her saying you both have gotten very close, and she's known one other team like you. [[spoiler: One was "an imbecile" she had to destroy, the other she doesn't elaborate on. Obviously, this is referencing Wheatley and Chell, and how they did have a friendship between each other as they tried to escape the facility, before Wheatley goes mad with power. Thanks for depressing me with the reminder that Wheatley is stuck in space, unable to apologize for what he did, game!]]
** Most of [=GlaDOS=]' comments in Co-op are sad when you realize she's saying things she told Chell in single player [[spoiler: Because she might miss her after all]]. Like the deer, the weight thing, ect. [[spoiler: Plus her reference about how human subjects are better doesn't help... She says it in a cruel way, but that's just how [=GlaDOS=] is. Tsundere much?]]
* In the final Co-Op level, when you [[spoiler: find the vault containing human subjects]] you see a reprogrammed turret which screamed [[spoiler: "I need to protect the humans!"]] The turret is quickly killed off by [=GlaDOS=] just so she could deploy blue gel (and possibly silence the turret).
* The way [=GlaDOS=] just casually crushes [[spoiler:Wheatley]] after waking up.
* The way [=GlaDOS=] screams while [[spoiler:you replace her with Wheatley]] may result in this, with a dash of [[WhattheHellHero What the Hell, Hero?]] since it's entirely your fault.
* Some of the alternate Cave Johnsons:
** The Space Warden Cave Johnson gets shived by criminals during a power blackout. He is actually in the process of dying when he addresses you.
** [=CaveDOS=], who, unlike [=GlaDOS=], begins to realize that BrainUploading himself was a terrible idea. He's still hilarious though.
---> (on Hercules) "[He] defeated all of Earth's monsters so humanity would be safe, and then went to Olympus for his trouble. ...Dammit. ''Death'' was my monster. And I killed it... Where's ''my'' Olympus?"
----