* In Chapter 3, Choko recounts what it was like to [[spoiler:witness Turbo's destructive hacking from within the game code itself]]. Particularly heartbreaking is how [[spoiler:she tried -- and failed -- to help the NPC King Candy]], which becomes even HarsherInHindsight when [[spoiler:the original is restored to life by the reboot and is revealed to be a MeanCharacterNiceActor]].
* Vanellope's breakdown in Chapter 5 after she discovers [[spoiler:''Sugar Rush'' was devestated by a virus and crashed, with all her friends corrupted and suffering horrific glitches to the point of being unrecognizable]].
* Ralph's reaction to having to watch helplessly as Vanellope [[spoiler:races Virus-Van]], followed by [[spoiler:''Sugar Rush'' resetting]] right before his eyes. This is immediately followed by him [[spoiler:racing to the game and tracking down Vanellope, only for her to have the doughnut guards escort this 'stranger' right back out]].
* The other effects of [[spoiler:Vanellope forcibly resetting ''Sugar Rush'']] are also tear-inducing:
** Minty Candlezaki and Sour Appleblossom becoming heated rivals, practically out to ''kill'' each other. (As well as the small but telling detail of [[spoiler:everyone calling her 'Minty' instead of 'Candle']].)
** Adorabeezle's awkwardness around Marzipanne, even as the [[PluckyGirl Plucky]] {{Cloudcuckoolander}} tries to befriend her.
** Snowanna being shunned by everyone but Gloyd for no real reason at all.
*** It's implied that Snowanna had [[spoiler:abandoned Vanellope at one point, possibly during the events of the movie]]. Still doesn't make her treatment any less sad, though.