* [[spoiler:Luciola's death]]. Having spent the whole series as an Extreme Doormat, his defiance of Delphine's will for the sake of Dio was truly inspiring. Then he demands that she truly set him free, and she asks what Dio is worth to him [[spoiler:just before killing him]].
-->[[spoiler:'''Luciola:''']] He... He is my friend, and I will not betray him.
** [[FromBadToWorse It got worse]] for the viewers once they learned the name of the incredibly heartrending song played during [[spoiler: Luciola's flashbacks of his earlier times with Dio, as well as during his death scene]]: "Lost Friend." This troper cannot listen to that song without crying.
* After this, [[spoiler: Lord Dio]] in the final episode. The reference to the earlier race where Dio shouted for Luciola to jump out and lighten the load in order for him to win [[spoiler: is mirrored when Dio, having beaten Claus, turns around to celebrate his victory with Luciola only to find that he's not there. This really drove home Luciola's death to this Troper.]]
* Additionally, Sophia [[spoiler: launching the attack on the Guild ship, only to hear Alex speak just as the attack was launched. Alex having killed the Maestro only moments before meant that the missile attack was meaningless, and he died at the hands of his second-in-command.]]
** Taken to another level when Sophia asks one of the bridge officers what Alex had said. The officer[[spoiler: lies, telling her that he had said her name. In reality, he had said "Eurys," the name of his long-dead wife. Presumably, Sophia goes on to live her life thinking that Alex loved her, when he really only cared about revenge.]]