* The dogs howling during the dog pound sequence.
** During the scenes in the pound, we see a dog being led off to another room, where it's heavily implied the dog was put to sleep. They never outright say it, but Lady turns to the other dogs and says "You...you mean he's...?"
* Trusty's [[spoiler: DisneyDeath]]. Jock whimpering, then when he nudges Trusty and there's no response, he looks up at the sky and lets out a mournful howl.
** It would've been even worse had the filmmakers gone with the original plan to [[spoiler:kill him off for real.]] Fortunately, they decided against it.
* Lady's TheReasonYouSuckSpeech to Tramp doubles as a CrowningMomentOfAwesome, but you can hear the sadness in her voice.
** Makes it doubly more saddening when Tramp leaves (because Lady tells him to) and you can see from his expression that he really fell in love with her.
* Lady's sad and confused reaction after Darling ''hit'' her. It wasn't anywhere close to animal abuse, but you can't help but feel pity for the poor girl.
** There's Lady's reaction to Jim Dear calling her "that dog". Jock and Trusty couldn't believe it when she told them about it, and she said that he'd never called her that before.