* [[spoiler:Shun giving his collar to Saki and saying good bye to his pet dog Subaru.]] ItMakesSenseInContext.
** Episode 8 in general is this. You have Mamoru ''desperately'' trying to get Maria's attention only to find his efforts in vain, Shun and Satoru [[spoiler:breaking off their perfectly healthy relationship because of Shun's SanitySlippage]], and the above example [[spoiler:which definitely foreshadows that something horrible is going to happen to Shun.]]
* Episode 9. Saki's mother. [[spoiler:"I don't want to lose any more kids... no, I just don't want to lose ''you!''"]]
* Episode 10. '''Oh God, Episode 10'''.
** Even before that climatic moment, there's Saki calling herself out on her inability to save [[spoiler:Shun]]. To see a 14 year old girl walk so close to the DespairEventHorizon is hard to watch.
** [[spoiler:Subaru's death to a Fujoneko]] was pretty saddening to watch as well, especially when [[spoiler:Shun takes a moment to reflect on his pet dog's HeroicSacrifice.]]
** On top of all that, [[spoiler:one would never expect Shun, of all people, to resign himself to his fate and feel so broken because of it. Seeing him do just that is ''heartbreaking.'']]
** Of course, all of these moments lead to the biggest TearJerker thus far in the series: [[spoiler:Shun's death.]] Seeing [[spoiler:Shun]] tear up before dying as Saki screams his name in horror is going to cause one to shed tears for a ''very'' long time.
--->'''[[spoiler:Shun]]''': Saki... I've always loved you. Goodbye... (gets swallowed by the Earth).
** Episode 10 is what caused ''Literature/FromTheNewWorld'' to have a TearJerker page. That pretty much tells how big of a TearJerker this episode is.
* Episode 11 doesn't have quite so much to sob at, but there's still something harsh about the fact that [[spoiler:Shun is only refered to as "X" ''even in the end credits'']].
* Episode 12 follows suit with Kutegawa Izumi, the first Gouma (Karma Demon) recorded in the village. An otherwise sweet, perfectly normal girl, her PK powers warp everything around her. [[DrivenToSuicide Watching her resort to suicide]] [[HeroicSacrifice to save the village]] is... depressing to say the least. Not to mention Saki's tears after Tomiko finishes the story.
** Oh, and Izumi's family? All mutated and killed by her unknowingly. You know it's incredibly sad when the Ethics Committee can't bring themselves to deliver the news of the death of Izumi's family to her.
** While [[spoiler:memories of Shun were erased from everyone after his death]], it's clear that Saki still remembers him subconsciously. As she hears about Izumi, she breaks down and doesn't understand why because she's unable to fully remember [[spoiler:Shun]].
* Episode 16: [[spoiler: Maria's final letter to Saki. All of it. From Creator/KanaHanazawa's superlative and emotional voice-acting to Maria's reasons for leaving Kamisu 66, it's brutally difficult not to cry.]]
** Also, the insert song (later revealed to be the series' second [=ED=]). Take a look at those lyrics and the images of [[spoiler:Maria and Saki playing as children]]. You won't last a few seconds before tearing up.
** "When I noticed that the two of us were skiing side-by-side, I realized what I had truly been afraid of. My world consisted only of Sage Academy, if one excludes my parents. And within that world, I could only call those of Group One my friends. [[spoiler:One by one those friends disappeared, until only Satoru and I were left. I hated it I didn't want to lose any more friends and loved ones... The two of us were alone in this world.]]"
*** As if that wasn't painful enough already, at one point [[spoiler:Saki starts to see Shun in Satoru's place as she follows him, and tries to reach out to him]]. In other words, this episode likes to remind us of the ''other'' major TearJerker ep on top of being very sobworthy in its own right.
** [[DarkReprise Dvorak's ''From the New World'' theme returns,]] only this time to signal the end of another [[spoiler:fruitless day of the two trying to find Maria and Mamoru. And, given Tomiko's deadlines, Satoru and Saki are out of time: '''Mamoru and Maria are gone for good.''']]
** As they rest in the makeshift igloo, Saki asks Satoru [[spoiler:if the two will forget Mamoru and Maria just like they did with Shun.]] Let the floodgates be opened.
** Basically the entirety of episode 16, from the note to the memories to the helplessness and lonesomeness that Saki and Satoru feel.
** The original novel explicitly describes the sex scene between Satoru and Saki that is only implied in the anime. In the middle of it Saki has a moment where she realizes that she and Satoru are probably imagining they are, rather than each other, making love to the boy they both loved, Shun, whom at the moment they can't even properly remember the name or face of. The notion may be uncomfortable to think about, but it's also ''heartbreakingly sad''.
* Episode 18: [[spoiler:Through DNA testing, Tomiko concludes that the bones given to them by Yakomaru in fact belonged to Maria and Mamoru. In other words, two more of Saki's friends are dead.]] It's not hard to see why Saki was so shocked after hearing the news.
* Episode 20: [[spoiler:Upon being critically injured, Tomiko knows she has very little time left before Yakomaru's army and his Akki come to finish Kamisu 66 off. Seeing Tomiko [[PassingTheTorch give her position to Saki]] before sending her and Niimi (Tomiko's assistant) off in spite of their protests is ''heartbreaking''.]]
* TheReveal in Episode 21. [[spoiler:That Akki? That bloodthirsty, nightmare-inducing Akki? '''SHE'S MARIA'S AND MAMORU'S DAUGHTER.''']]
* In episode 23, Saki finally [[spoiler:remembers Shun]] in the backdrop of Tokyo's ruins and [[https://www.youtube.com/watch?v=EMabpnIqc3w this awesome piece of BGM]]. The entire sequence is definitely one of the most powerful moments of this series.
* Episode 25: Admit it, you cried during [[spoiler:Kiroumaru's DyingMomentOfAwesome.]]
** To say nothing of [[spoiler:the death of Mamoru's and Maria's daughter. Groomed to be Yakomaru's secret weapon, this girl will never have an opportunity to live a normal life, go to school, or even know who she was in the first place. The fact that [[StrongFamilyResemblance she greatly resembles her mother]] doesn't help at all.]]
-->'''Saki''': [[spoiler:[[WhatCouldHaveBeen If she had been born in the villages like the rest of us, I'm sure she would've grown into a lovely and talented girl.]] Though I know it was not meant to be... [[DyingAsYourself I still wish she could have breathed her last as a human being.]]]]
** The montage of flashbacks from previous episodes involving [[spoiler: Maria and Mamoru after their daughter dies in the anime]] accompanied by Original Sin playing in the background is nothing short of heartbreaking especially after learning [[BusCrash what happened to them]].
** [[spoiler:[[KangarooCourt The entire trial]] [[AlasPoorVillain of Yakomaru.]] Yes he might have been the villain, but hearing the crowds ignore and mock him and the court issuing a FateWorseThanDeath goes to show [[HumansAreBastards how wicked the human beings in this show really are.]]]]
-->'''[[spoiler:Yakomaru]]''': "We are not beasts nor slaves. ...'''WE ARE HUMANS!!!'''"
** Made incredibly worse when [[spoiler:you realize the truth about the Bakenezumis' origins.]]
** Saki speaking to Squealer one last time, reminiscing about when they first met, before ending his suffering by mercy killing him.
* A fan-art example: [[https://static.tvtropes.org/pmwiki/pub/images/102f6865f10afb7823e296011e98b02a.jpg One fan-made wallpaper]] depicts Mamoru and Maria [[spoiler:playing with their kid. Tears will be shed, especially if you know that Mamoru and Maria were murdered not long after their child's birth and thus would never have the chance to do just that. Knowing what Yakomaru did to their daughter only worsens the tears.]]