* [[spoiler: Joker's death. He was a terrible, lunatic murderer, and anyone who says that he deserved to die has a solid argument. But the magnitude of the situation, the slow pacing, the quiet credits, and what happens in the middle of the credits, there's such an emotional impact. [[HarsherInHindsight And when you realize that this may well be the last time we hear Mark Hamill's Joker]], it really does feel like something important, something you've known since childhood, really did die.]]
** [[spoiler:And it's not just his death, but his final moments leading up to it. Right after his last laugh and his IncurableCoughOfDeath, his body suddenly starts jerking as he clutches his chest and makes very loud, painful gasps, indicating that the Titan poisoning has completely taken over his bloodstream. The gasps start slowing down and getting quieter as seconds pass, until, at his final gasp, he quietly lets out his long, last breath as he [[GoOutWithASmile goes out with a smile]]. Combined with NightmareFuel, nothing says "painful sadness" like his last moments.]]
** [[FamousLastWords "That actually is... pretty funny..."]]
** What really makes you wanna cry is how [[spoiler: none of the Joker's gang attacks Batman when he carries Joker's body out of the theater. They all just stand back, getting out of Batman's way, and watch, shocked (and appropriately so).]]
** Heck, the music alone is enough to make you weep. The name of the song in the soundtrack? Four words: It's. Not. Even. Breakfast. The song starts seemingly normal and dependent, but then shock starts coming in at around the 1:20 mark. Over a minute of unease starts settling in, and then, at around the 2:35 mark, the waterworks start on emotionally. Truly a [[SugarWiki/AwesomeMusic Crowning Music of Sadness]]. [[http://www.youtube.com/watch?v=Ej8RILZ9dTo Give it a listen.]]
** Knowing that this is the last time Hamill would voice the Joker, "Only You (And You Alone)" feels as much like Hamill singing his farewell to his fans as it does Joker singing to Batman. It's a beautiful send off for this era of the Joker.
* During the PlayableEpilogue, [[spoiler: walking up to any of the Harley statues and activating it plays her message: sobbing uncontrollably.]]
** Watch the reactions of the members of the Joker gang gathered around the theater as [[spoiler:Batman walks out carrying Joker's body. Harley just... collapses]].
* One of the Riddler's interview tapes has him imply Hugo Strange has a Batsuit hidden behind a secret panel in his office, that he sometimes wears in private, sobbing into his hands and wondering if he's really worthy to replace Batman. It does a good job of hammering home how, despite his bluster, Hugo Strange is a pretty pathetic person.
** Or, in another frame of reference, how unbelievably strong ''Batman'' is, to have kept it up for so long.
* [[http://www.youtube.com/watch?v=eHRCseft5N8&feature=related Paying your respects to Bruce Wayne's parents.]]
** The music in general, [[http://www.youtube.com/watch?v=-3IbrRfxK5Q "A Rose for Respect"]], played when you pay your respects. When played again at the [[http://www.youtube.com/watch?v=wLb6iDCvcfA end credits of "Harley Quinn's Revenge"]], it takes on a whole new meaning, [[spoiler:as it turns into a sad requiem for Talia and the Joker]].
** The scene as a whole beautifully describes just who and what the Batman is, a terrifying creature whose sole purpose is to prevent the very thing that created him. One can't help but shed a few tears as Bruce/Batman mourns in what is one of the most poignant scenes in the game.
* Batman's [[spoiler: hallucination of his parents standing behind a white light when he's close to death from the Titan blood]].
** Immediately afterwards, Oracle freaks out as Batman is only minutes from death and begs him to tell her what to do, what to tell Robin. [[{{Determinator}} His reply is that he'll make it.]]
* The death of [[spoiler: Talia al Ghul]]. The pain in Batman's and [[spoiler: Talia's]] faces as [[spoiler: she]] dies is pretty sad. [[spoiler: Joker's]] KickTheDog TooSoon line is somehow both funny and enough to fill a player with hate-filled vengeance towards the villain due to the sadness of that scene. [[spoiler: Talia probably isn't KilledOffForReal because of the Lazarus Pit, but it is still sad.]]
* [[spoiler: Harley's pregnant.]] While this first seems like good news for her, there's a lot that makes it very likely more of a TearJerker. [[spoiler: Considering the baby was conceived while Joker was sick, if the Titan has the ability to be based on to offspring, the baby could easily be deformed in some way; it's more than likely that the baby will be taken away from Harley after it's born; and, worst of all, who's to say she'll even give birth to it? The pregnancy test was no doubt taken sometime before the start of the game, and she couldn't be too far along. A lot of the things that we can assume happen to her could have resulted in miscarriage. Plus the possible Titan-based damage present in the baby itself could cause the baby to die before it's born.]]
** Titan isn't the only thing in Joker's body during Arkham City. He's always had traces of the original Joker Venom in his veins, as well.
** The new DLC hints that [[spoiler:the pregnancy test might have given her a false positive]]. This coupled with the fact [[spoiler:Joker's dead and she has a gang she has no idea how to run (and none of them can stand her)]], is tragic in its own way, psychopath or not.
* Hearing Victor Fries give [[http://www.youtube.com/watch?v=b3bw9HrjyPk insight into how he feels about his past]] is quite heartbreaking.
-->'''Freeze''': Have you ever seen a flower die? Watched something that was once so beautiful, so full of life, collapse and rot from within?
** Hell, Mr. Freeze in general.
** Do you know what the title of his [[spoiler: boss theme]] is? "Bring Her Back To Me." ''Ouch.''
* After you defeat the Mad Hatter you can enter his lair where Batman will tell him never to mess with his head again. The hatter replies that all he wanted was a friend and bursts into horrible sobbing that is truly heartbreaking to listen to. Listening to his interview tapes, he is a killer but it is also obvious that the poor man is completely delusional and needs serious help which he's not going to get. Hearing Hugo Strange manipulate the poor bastard into helping him is also horrible.
* When you're in the museum and you see the exhibits Penguin has set up. One of them contains the taxidermized hyena pets of Harley's/Joker's. Harley LOVED her babies, and Penguin just murdered them and set them on display.
* The gloomy music outside the Steel Mill in "Harley Quinn's Revenge" is more depressing, [[spoiler:as it takes on a whole new meaning after the Joker's death by Titan poisoning.]]
** Also, when Batman speaks or thinks to himself in the flashback, just hearing him is more depressing, as you can hear the pain, tiredness, anger and sadness in his voice. It's like he hasn't sounded the same [[spoiler:as when the Joker was still alive. In fact, it's almost like he hates himself for breathing without the Joker, as if he may never get over the death of his greatest enemy as well as that of Talia. After all that he has gone through, you feel like you wanted to give him a hug so badly]].
* The ending of the comic ''Arkham City: Endgame''. [[spoiler:Just before the [[DueToTheDead process of cremation]], Batman takes one final look at the Joker's corpse. Losing the Joker to the disease has taken its terrible, emotional toll on the Dark Knight for so long, and it's taking a long time to heal and find closure; but in the end, he finally learns to let go of the bond between them and move on by saying his last goodbye to his longtime foe. So emotionally sad that the Clown Prince of Crime is truly never coming back in the Arkhamverse, but, in the words of Music/{{Madonna}}, there ''is'' no greater power than the power of goodbye.]]
--> ''"No more laughs."''