There are times when Atomic Betty had its [[TearJerker moments of sadness.]]
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* "Galactic Guardians No More", when the guardians officially break up (before reuniting later in the end of this episode), Betty separating from Sparky and X-5.
* [[spoiler:Paloma's life source being drained.]]
* The scene where Maximus [[spoiler:recalls how he was treated by the Felinians back when he was a baby. We see the baby kitten being shunned by many of them, trying to make friends and crying]].
* Maximus' implied abuse from his father.
* Penelope mourning the death of her pet cat.
* Another one that counts is Paloma leaving Earth near the end of the first Mission: Earth episode. Seeing her go made you really feel that you want her to come back, but she couldn't (save for a cameo appearance).
* The song, "Hold On" from the album.
* These scenes in "The No-L 9":
** Beatrixo's story about how her husband Jimmy went missing.
** Betty realizing the man she's run into on the No-L 9 is her long-lost grandfather.
** Beatrixo reuniting with Jimmy after 30 years.
** Mrs. Barrett reuniting with her father.
** The ending song that goes along with this special.