There are times when Atomic Betty had its [[TearJerker moments of sadness.]]
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* "Galactic Guardians No More", when the guardians officially break up (before reuniting later in the end of this episode), Betty separating from Sparky and X-5.
* [[spoiler:Paloma's life source being drained.]]
* The scene where Maximus [[spoiler:recalls how he was treated by the Felinians back when he was a baby. We see the baby kitten being shunned by many of them, trying to make friends and crying]].
* Maximus' implied abuse from his father.
* Penelope mourning the death of her pet cat, Poopsie. Even if you don't like her, anyone who lost a pet would sympathize with her.
* Another one that counts is Paloma leaving Earth near the end of the first Mission: Earth episode. Seeing her go made you really feel that you want her to come back, but she couldn't (save for a cameo appearance).
* The song, "Hold On" from the album.
* These scenes in "The No-L 9":
** Beatrixo's story about how her husband Jimmy went missing.
** Betty realizing the man she's run into on the No-L 9 is [[spoiler:her long-lost grandfather]].
** Beatrixo reuniting with [[spoiler:Jimmy]] after 30 years.
** Mrs. Barrett reuniting with [[spoiler:her father]].
** The ending song that goes along with this special.