-->'''Rachel Alucard''': What possible point is there to a ghost that isn't awake at night?
-->'''Ragna the Bloodedge''': ''[[WhyDidItHaveToBeSnakes GHOST?!]]'' *[[ScreamsLikeALittleGirl tropes]]*
--->-''VideoGame/BlazBlueContinuumShift'' Gag Reel

* Deadpool occasionally screams like this when hit in ''VideoGame/MarvelVsCapcom3''.
* Baba from ''VideoGame/{{F-Zero}} X''; a male character who used the female scream sound-byte when falling off the track. Stopped in ''F-Zero GX'', but got [[CampGay no less effeminate]].
* Francis from ''VideoGame/Left4Dead'' will scream like this as well whenever he's pounced by a Hunter or attacked when downed. Kind of funny coming from a guy who exclaims a constant macho, "I hate _________"
* X's death scream in ''VideoGame/MegaManX 4'', thanks to abysmal voice acting.
* ''VideoGame/TeamFortress2''
** In "Meet the Sandvich", the Scout screams like a little girl in the ending, thanks to the Heavy having ''[[BloodyHilarious having punched out all his blood.]]'' Played for laughs, of course.
** An in the Halloween event maps, getting spooked by either the Horseless Headless Horsemann or Zepheniah Mann's ghost will result in very unmanly screaming regardless of class.
** During the ending of "Meet the Pyro", [=BLU=] Sniper screams like a girl as Pyro puts him out of his misery.
** In "The Sound of Medicine," RED Scout screams like a terrified strangled bird upon spotting a Tank Robot and waves of Soldier-Bots.
* ''VideoGame/SamAndMaxFreelancePolice'':
-->'''Max:''' Mind if I drive?\\
'''Sam:''' Not if you don't mind me clawing at the dash and shrieking like a cheerleader.
** Also, in first episode of the second season, [[ConspiracyTheorist Bosco]] screams like this in response to the word ''them''. Sam even gets a dialogue option to repeatedly say "Them!" just to freak him out. [[spoiler: Admittedly, Bosco has a very good reason to freak out, as we learn in the last episode of the season.]]
* In ''InfiniteSpace'', Ian apparently did this offscreen when he and Torlo evacuated Rhea (whom they thought dead until she suddenly moved). He is not pleased when Yuri and Torlo mock him about it.
* ''VideoGame/BlazBlue''. If you use the English voice, in Ragna's Gag Reel, after being informed that he's a ghost, the very thing he feared, he screams like a little girl. There's no girly scream in the Japanese voice, though.
* ''GrimFandango'':
-->'''Glottis:''' There was this one high-pitched whine it was making -- really grating noise, you know? And I searched and searched, but I couldn't find the source of the noise, until we pulled in here.\\
'''Velasco:''' Was it the blower?\\
'''Glottis:''' No, it was Manny screaming in the back like a cat tied to a cruise missile!
* ''VideoGame/{{Blood}}''. Caleb screams like a little girl when he dies. Or is set on fire.
-->'''Caleb:''' ''AH! IT BURNS! AH AH AH AH AAAAAH!''
* ''VideoGame/DragonsLair''. Dirk the Daring does this a number of times, but most noticeably in one "miss" sequence during the "Crypt Creeps" scene with what looks like black goo.
* The concierge from ''VideoGame/GhostbustersTheVideoGame'' screams like this, which the Ghostbusters at first mistake for a ghost.
* ''VideoGame/AssassinsCreedBrotherhood'': [[BigBad Cesare Borgia]] of all people does this when Ezio [[spoiler:hurls him off a rampart in Viana.]]
* Alistair of ''VideoGame/DragonAgeOrigins'' confesses that he used to do this while dreaming of darkspawn and the Archdemon: "I screamed like a little girl. Duncan thought I had someone in my room." He also demonstrates girly screaming in a banter with Shale, attempting to give [[{{Golem}} Shale]] an idea of what pain feels like.
* Elh of ''VideoGame/{{Solatorobo}}'' hates [[WhyDidItHaveToBeSnakes bugs]] and screams when they get too close. [[spoiler:Of course, given that she ''is'' a girl, it's more understandable, but Red didn't know that at the time.]]
* Murphy from ''VideoGame/SilentHillDownpour'' tends to do this. Not that we blame him.
* ''PennyArcadeAdventures: On the Rain-Slick Precipice of Darkness'' does this three times:
** In Episode 1, Gabe makes a girlish scream before beating on the fortune-telling machine.
** In Episode 2, Gabe screams like a girl when he is locked inside the asylum.
** Also in Episode 2, when Gabe, Tycho, and the player escape from the apartment, Tycho screams like a girl upon noticing that the building is crumbling down.
* Garry from ''{{Ib}}'' screams this way-- despite having no voice acting, the game's text blatantly describes the sounds of his screaming as "eeek!" or some variant thereof.
* Punch out [[CampGay Ash]] in ''StreetsOfRage'' and you learn he has the female death cry.
* ''VisualNovel/SuperDanganRonpa2'':
** Kazuichi Souda does this a lot, since he gets freaked out at the at the slightest provocation.
** In Chapter 1, [[spoiler:Teruteru Hanamura]] does this [[spoiler:when Hinata figures out that he's the culprit of the fake Togami's murder.]]
* Booker [=DeWitt=] of ''VideoGame/BioshockInfinite'' does this whenever he is hit by a fire attack. [[JustifiedTrope To be fair]], being burned ''really hurts''.
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