* [[Creator/TomokazuSugita Tomokazu Sugita]] has the penchant on this trope in Two characters in a different anime:
** Kyon's theme song from ''Literature/HaruhiSuzumiya'' includes one of these for no apparent reason (he NEVER does this in the manga or anime).
** Gintoki of ''Manga/{{Gintama}}'' fame occasionally lets out a comical scream of this nature when he's subjected to something he finds particularly horrifying. If paired with a GroinAttack, it is PlayedForLaughs. Said incidents include:
*** Finding a giant-sized cockroach with Kagura.
*** Being in Kanashibari before the anime.
*** Getting a ride from [[DrivesLikeCrazy Katsura]], who ignores an s-curve by driving up straight.
--->'''Gintoki''': S-curve, and you don't give a damn?!
*** Listening to an otherwise relaxing radio story that caps off with a SurprisinglyCreepyMoment.
*** Getting his balls pixelated by the Demon King.
*** [[GroinAttack Taking Gedoumaru's kanabo to the crotch]] right after [[CurbStompBattle performing an]] [[WrestlerInAllOfUs aerial ass bomber]] on Doman before he teleports.
*** Later, getting his balls detached by Doman immediately after Seimei reattached them in the aftermath of the previous GroinAttack.
*** Provoking Kagura's wrath by mistaking her being bashful about giving him Valentine's chocolate for her being constipated.
*** Playing [[Literature/TheRing a cursed Blu-Ray disc]] in episode 224.
*** And by extension, seeing [[Creator/MiyukiSawashiro BluRayko]] becomes a GPS only to locate a kotatsu by meters.
*** And in [[CheerfulFuneral Episode 231]], getting his nuts grabbed by the owner's wife after saving the corpse of her husband at the truck.
*** Here's a [[https://youtu.be/kfT9hudIphs compilation]] of his screams.
* Creator/YoshitsuguMatsuoka is no slouch in this area himself, being able to emote an absolutely adorable screech of girlish-terror for comedic purposes. Just listen to him in [[https://www.youtube.com/watch?v=g6Yvmx8Eq0Q&t=344s the comedic Blu-Ray DVD extras "Sword Art Offline]]" and in "[[https://www.youtube.com/watch?v=e3rJyjo6SBM Is it wrong to pick up girls in a dungeon?]]"
* ''Anime/CodeGeass'': Even anti-heroes scream in high-pitched [[https://www.youtube.com/watch?v=tiwYeihYwgw voices]]
* In ''Manga/SoulEater'' Black Star lets out a high-pitched squeal when Dr. Stein catches him trying to sneak a peek at the answers for a big test. The dub version in particular sounds quite girlish compared to the original Japanese.
* Nozomu's "[[BigNo IIIIIIYYYYYYAAAAAAA!!!!!!]]" from ''Manga/SayonaraZetsubouSensei''. One of the funniest instances is when he does this after the girls see him naked during the HotSpringsEpisode, and the DeadpanSnarker Abiru lampshades that "that should be our reaction".
* [[TheDitz North Italy]] in ''Webcomic/HetaliaAxisPowers'' at one point screams "Kyaaaa!". [[HeroicWannabe America]] as well.
** Greece in the dub of season 3, when Japan pretends to pull his thumb off.
** Romano shows off his manly screech in the dub, when both he and his brother are caught by England. Actually, he does this a lot.
** Finland in the dub of season three too, from the "Running Away with Mr Sweden" episode.
* Zoisite does this when he discovers that he is surrounded by rats in the original Japanese version of ''Anime/SailorMoon''. Aside from his relationship with Kunzite, this was one of the main reasons he was turned into a girl for the Creator/DiC version.
** Artemis does this in the Viz dub, courtesy of Creator/JohnnyYongBosch.
* Early in ''Manga/AzumangaDaioh'', a war against a cockroach begins after a very high-pitched scream erupts from one of the students. Afterward, it's revealed the source of that scream was Sakaki who, while being an actual girl, is also an AloofDarkHairedGirl and normally has a deep voice.
* Ramiel, an EldritchAbomination made from impossible geometric shapes, in ''Anime/RebuildOfEvangelion''. ''Definitely'' not PlayedForLaughs.
* In the ''[[Anime/PokemonTheSeries Pokemon]]'' anime, Cilan lets out a [[https://www.youtube.com/watch?v=qze5PJ7l0KQ very girly scream]] in one episode.
* From episode 18 of ''Anime/YuGiOhGX'':
-->'''Jaden:''' ''(Crowler screams offscreen)'' Someone's in trouble!\\
'''Bastion:''' Let's go help her!
* In any {{Ecchi}} anime, whenever the guy walks in on a girl taking a bath, you'd expect the girl screaming at the guy. This is not the case in ''Literature/HighSchoolDxD'' when Issei walks in on the bathroom while Asia was still inside. ''He's the one'' screaming and trying to cover himself instead of the other way around.
* In ''Manga/ExcelSaga'', when Iwata [[ItMakesSenseInContext finds himself in Ropponmatsu II's]] body, his first reaction is to proclaim "[[TropeName HIGH PITCHED GIRLISH SHRIEK]]!!"
* Tubby screamed this way in most episodes of the ''Anime/LittleLulu'' anime, most notably in Episode 2.
* Mamoru from ''Literature/FromTheNewWorld'' does this in episode two, and Maria scolds him about it.
-->'''Maria:''' Don't cry like a little girl!
* In the anime adaptation of ''Literature/IsItWrongToTryToPickUpGirlsInADungeon'', Bell sees Aiz and tries to escape her, only to roll face-first into her butt. His scream of embarrassment has to be [[https://www.youtube.com/watch?v=TR93f4XZGJQ heard to be believed.]]
* Tsuna from ''Manga/Reborn2004'' as well, where he definitely screams like a little girl in almost every surprise that Reborn will throw at him. Oh and little puppies too. Expect him to scream every single episode
* In ''Manga/DemonSlayerKimetsuNoYaiba'', part and parcel with his NoIndoorVoice and LargeHam personality, Zenitsu ''screams'' every emotion on the top of his lungs, especially when he is terrified. It's a testament to the skill, comedic timing and vocal-chord strength of Creator/HiroShimono that he has not lost his voice after his ''introduction'' arc.
** To a lesser extent, our leading protagonist Tanjiro's Japanese Voice Actor Creator/NatsukiHanae also emotes the most adorably ''girlish'' screams whenever he is [[RunningGag fleeing from Mr Haganezuka for breaking yet *another* one of his swords.]] Though to be fair, ''any'' boy would probably screech in fright if he was [[SlasherFilm chased by a 6 foot tall musclebound maniac in a mask waving a kitchen knife.]]
* ''Manga/AyakashiTriangle'': Matsuri [[GenderBender was change from a boy to a girl]], and some [[ThirdLawOfGenderBending particularly emasculating]] moments are highlighted by undignified squealing, if not blatantly sexually moaning. The first time it happens, after Suzu [[ThanksForTheMammaries accidentally grabs his breasts]], he's even surprised by the specific sound he made ("hyan" in Japanese, "meep" in English). Amusingly, after Suzu realizes [[IfItsYouItsOkay she likes Matsuri even as a girl]], she's ''{{aroused|ByTheirVoice}}'' by them.
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