Mr. Freeze returns, but this time, he is focused in destroying everyone's possessions. This is because his wife left him, believed he died ever since the events of ''Batman and Mr. Freeze: Subzero''. It's up to Batman and Batgirl to stop him form his latest scheme: freeze all of Gotham.
* BigOMG: Batgirl says this upon seeing Freeze in a robotic spider body.
* DespairEventHorizon: Mr. Freeze hits this so hard.
* HeyItsThatVoice: Ice Maiden #3 is really [[WesternAnimation/CodenameKidsNextDoor Numbuh 3]].
* RelationshipVoiceActor: The first time TaraStrong and LaurenTom worked together in.
* RevengeByProxy: Mr. Freeze's M.O.
* TrainingFromHell: Batgirl gets this one when Bruce increases her training difficulty level into 10 that he leaves her getting almost shot by the lasers.
* YouHaveFailedMe: Just how Mr. Freeze left one of his ice maidens behind after her ankle got frozen.