The episode opens with a victim being chased by a hidden aggressor. Eventually, the victim can't run anymore, and, after a short back-and-forth with Poison Ivy, is shot with a strange gas. A short time later, Bruce Wayne receives an invitation to a retreat at the Eternal Youth Spa. Per the norm, he turns it down, but suggests that Alfred and his lady friend take it instead. When Alfred comes back, he seems strangely more plant-oriented, and a trip to the Spa uncovers a horrible secret.
!! This episode contains examples of the following tropes:
* BlondeBrunetteRedhead: Lily, Violet, and Ivy.
* BodyHorror: The episode has this in spades for the victims of the villain, mixed with AndIMustScream.
* BoundAndGagged: Lily and Violet.
* CutLexLuthorACheck: Poison Ivy somehow managed to afford a gigantic mansion with its own power plant and extensive grounds in order to set up a fake health spa for one episode... complete with a staff of women loyal enough to kill on her part and try fighting the Batman, and a greenhouse full of extremely rare, nearly extinct, fully-grown trees found only in the depths of the Amazon.
* GirlPosse: Ivy's two henchgirls Violet and Lily.
* LongevityTreatment: Poison Ivy under the guise of Dr. Demeter offers this treatment to rich industrialists but in reality is turning them into trees as karmic justice for their environmental destruction. She has no qualms about going after their friends or loved ones as well.
* OutOfCharacterAlert: Alfred putting extra plants around the house and Batcave tips Batman off that something is wrong.
* RevengeByProxy: Of a sort. Poison Ivy transforms Alfred and his girlfriend not to get to Bruce, but because "someone has to pay for [his] crimes."
* RevengeMyopia: Ivy. Not only is killing plants not tantamount to killing humans, but also Wayne was only distantly in charge of the operation for which she tries to punish him and was not any more pleased about the ecosystem damage (when he heard of it) than she was.
* SayMyName: "Alfred? Alfred! ''Alfred!!'' '''''ALLFREEEEEDDD!!!!!!!!'''''"
* SpeakInUnison: Violet and Lily. As Alfred pointed out while watching the video.
* WellIntentionedExtremist: Even Wayne is concerned about the effects of corporations on the environment, but Ivy takes it to an extreme level.