[[folder: Get your hands off me! No! Stop! No! NO! NO, NO, NOOOOOOOOOOOOOOOOOO! ]]
-->-- '''[=GLaDOS=]''', during [[spoiler: the core transfer]], ''Videogame/Portal2''

->''"NOOOOOO!'' '''''EN-OH-OH-OOOOOOOH!!'''"''
->''(In retrospective) "Funny story, the script called for me to say '[[BigYes Yes]]', but I gave it a little twist."
-->-- '''Calculon''', ''WesternAnimation/{{Futurama}}'', "Bend Her"

-->-- '''Edgeworth''', ''VisualNovel/AceAttorneyInvestigationsMilesEdgeworth''

->'''"NO! NO! [[LargeHam NOOOOO!!]]"'''
-->-- '''Froggo''', ''WesternAnimation/{{Histeria}}!''

->'''"The short answer is no. The long answer is... NOOOOOOOOOOOO!!!!"'''
-->-- '''Strong Bad''', ''WebAnimation/HomestarRunner'', [[http://www.homestarrunner.com/sbemail203.html "independent"]]

->''"'''NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!''' [[DepartmentOfRedundancyDepartment By which I mean I cannot do it.]]"''
-->-- '''Mojo Jojo''', ''WesternAnimation/ThePowerpuffGirls'', "Mo’ Linguish"

->''"Gators in Equestria? I wonder what the adults look like. [[WesternAnimation/MyLittlePonyFriendshipIsMagic Zecora]], '''nooooo!'''"''
-->-- '''Saberspark''', My Two Bits: [[Recap/MyLittlePonyFriendshipIsMagic Party Of One]], Website/YouTube

-->-- '''Michael Scott''', ''Series/{{The Office|US}}'', "Frame Toby"

->''"Nooooooooooo (19 episodes later...) ooooooooooo!"''
-->-- ''Dragonball Peepee''

->''"NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!" screamed Baka until he passed out from lack of breath.''
-->-- '''Plan 7 of 9 from Outer Space'''

->''"I want to shout! I want to cry out so loud the entire world will hear! Very NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!"''
-->-- '''Pedro''', ''Manga/ExcelSaga''

->''"1UP" ''(the OneUp is blown up by the player)'' "[[WhatTheHellPlayer NO!]]"''
-->-- ''Cardinal Sins: VideoGame/JudgementSilversword Recycle Edition''

->''"[[OverlyLongGag Please continue! Please continue! Please continue! Please continue! Please continue! Please continue! Please continue! Please continue! Please continue! Please continue!]] '''NOOOOO!'''"''
-->-- '''The Announcer''', ''VideoGame/ArmedPoliceBatrider''

->''"I've got sand up my NOOOOOOOOOOOOOOOOOOOOSE!"''
-->-- '''Darth Vader''' after crashing on Tatooine, ''VideoGame/LegoStarWars''

->''"'''[[GratuitousJapanese YAMETEEEEE!!!]]'''"''
-->-- [[VideoGame/LeagueOfLegends Various champions]], ''WebVideo/LaLaLaDemacia''

-> '''NO!''' No! NO! ''NOOOOOO!''' Nonononono! EHN-OH-CAPITAL-CAPITAL-EXCLAIMATION-POINT! '''''NO!'''''
-->-- '''Amber''' after being told to deliver something to [[SitcomArchNemesis Lettering Guy]], ''{{Webcomic/Matchu}}''

->([[Disney/TheLionKing Mufasa]]'s spirit shows up in the clouds.)\\
'''Mufasa''': Vegeta.\\
'''Vegeta''': Mufasa?!\\
'''Mufasa''': You are a tool.\\
-->--''WebAnimation/{{Ducktalez}} 7''

->[[InvertedTrope "N]][[LittleNo o."]] *ding*
-->--'''Jeremy Scott''', WebVideo/CinemaSins

->''I opened my eyes and then saw a scene that will probably haunt me the rest of my life. I saw nine white [=EVAs=], circling around what was left of Headquarters. And in their hands, or even worse, their mouths, they held the remains of what had once been Unit-02.''\\
-->--'''Shinji''', ''Fanfic/TheOneILoveIs'', ''chapter 12''

->'''''"OOOH NOOOOOOO!!!!"'''''
-->--'''Joseph Joestar''', ''Manga/JojosBizarreAdventure''. In GratuitousEnglish.

->"NOOOOOOOOOOOOO! No! When I say no, you turn back around!"
-->-- ''Series/DoctorWho'', "The Doctor Falls"